Economie circulaire sur la valorisation des biomasses.

1. Integrated cascades of PROcesses for the
extraction and valorisation of proteins and
bioactive molecules from Legumes, Fungi and
Coffee agro-industrial side streams
17 November 2020 – Luxembourg Creative
Job Tchoumtchoua, PhD
R&D Project Leader

2. © CELABOR SCRL
Your multi-skilled scientific and technical partner
Expertise – Quality – Speed – Confidentiality
2

3. © CELABOR SCRL
What is CELABOR?
3
SME offering scientific and technical services
• Limited liability company (SCRL)
• Research Centre approved by the Walloon Region
• Created in 1995 by:
– Chamber of Commerce of Verviers (CCILV)
– Centexbel
– Wallonia
• Culture of a Research Centre and an SME
3

4. © CELABOR SCRL
What is CELABOR?
4
A multidisciplinary team of 50 people
• PhD, Engineers, Masters, Bachelors…
• Scientific backgrounds
Two locations
• Chaîneux (Herve) and Mouscron
Dedicated laboratories
4

5. © CELABOR SCRL
Activities
5
• Testing and analyses
• Expertise and technical advice
• Research and Development
• Training
5

6. © CELABOR SCRL 6
Mission
6
To promote technical innovation
Research and Development projects for companies
To meet the customer needs for testing and analysis
Large range of tests and analyses, lab qualified according to ISO
17025, specific method development on request
To solve problems and disputes
Independent expertise
To disseminate some know-how
Professional training
6

7. © CELABOR SCRL
Quality
7
ISO 17025 accreditation
Internationally recognised system
Approved by the Environment Ministry
7

8. © CELABOR SCRL
Customers
8
• SME
• Large national and multinational companies
• Retailers
• Administrations
• Customer associations
• Courts (expertises)
…
8

9. © CELABOR SCRL
Our know-how
Biomass and
Waste
Valorization
Platform
Nutrition
Extraction
Biomaterials
and Packaging
Environment
Textile
Food
Technologies
Platform
9

10. © CELABOR SCRL
Biomass Valorization Platform
Flexible Pilot Platform for Biomass Valorisation
Green extraction & purification
platformfor biomolecules recovery
Our extraction & downstream processing team has a broad blend of
skills and industrial backgrounds spanning the field of natural
substances extraction, fractionation and purification. This breath
Advanced biopolymers production &
greenfunctionalization
We offer solutions for producing cellulose and chitin nanofibrils with
controlled size by mechanical fibrillation combined to advanced
pretreatments. We are developing industrially robust processes for 10

11. © CELABOR SCRL
Extraction and Biomaterials department - Complementarities
From a selected biomass ...
Extraction and purification of biomolecules → building blocks - bioadditives
Biopolymer and fiber extraction → Lignin, cellulose, hemicellulose, chitin, proteins…
Fiber processing and functionalization → nano fibrillated products
Green surface modifications → functional surface
… to Application sectors
cosmetic
building textile
packaging
food
feed
pharmaceutic
nutraceutical
Extraction department
Biomaterial department
11

12. © CELABOR SCRL
Extraction Expertises
Microbial
products &
building-
blocks
recovery
EXTRACTION DEPARTMENT
12

13. © CELABOR SCRL
Technological platform
Three pilot plants Supercritical Fluid Extractors SFE-CO2
(2x 6L/batch)
Pilot-scale Subcritical Water Extraction (SWE) (6L/batch)
Conventional solvent extraction (60L and 350L)
Pilot-scale Pulsed Electric Field (S/L treatment)
Ultrasounds & Microwave Assisted Extraction (25L UAE/
3L MAE)
Lab and pilot-scale membrane separation (Ultra-filtration,
Nano-filtration
Pilot-scale post-treatment equipment (Freeze-dryer, Spray-
dryer, Evaporator, Centrifuge)
Purification platform (CPC, MPLC, Prep-HPLC)
Advanced analytical lab (UPLC-MS, GC-MS, ICP-MS, HPLC-
DAD-ELSD
Technological platform unique in Wallonia
ATEX zone
13

14. © CELABOR SCRL
What we can do
0 2 4 6 8 10 12 14
Time (min)
Rosmarinicacid
Carnosicacid
Carnosol
Pre-treatment and
extraction of raw
material
Fractionation and
Purification
Post-treatment,
concentration,
drying
Characterization
and quantification Qualification and
stability study
14

15. © CELABOR SCRL
Research Programs & Collaborative Projects
NENU2PHAR
MODEL2BIO
AQUACOMBINE
Celabor Full BIC member
15

16. Project Overview and
the specific role of Celabor

17. Project key figures
PROLIFIC at Luxembourg Creative!17 17 November 2020
Research & Innovation Action
Project duration Total Budget
4 years
5.3 million
END
2022
START
2018

18. Partners
18
8 European countries,
Cyprus, France, Germany,
Greece, Italy, Spain,
Switzerland
Coordination:
10 SMEs
3 large
4 RTD institutions
PROLIFIC at Luxembourg Creative!17 November 2020

19. PROLIFIC will valorize agro-industrial and
food processing residues
19
Food Waste
2 bn. tones/year!
Source of compounds
proteins, fibres etc.
Innovative Processes
proteins, fibres etc.
High protein demand
For food and feed
PROLIFIC at Luxembourg Creative!17 November 2020

20. PROLIFIC feedstocks
20
Coffee
Silverskin and
non-compliant green
coffee beans
Legumes
Non compliant:
• fresh pea seeds,
• fresh green beans,
• rehydrated chickpea seeds
Fungi
Processing cuttings, left overs and
mycelium of
• Agaricus bisporus,
• Pleurotus ostreatus,
• Lentinus edodes
PROLIFIC at Luxembourg Creative!17 November 2020

21. Relevance in Europe
Annual production 2017 –
FAO data
21
1.29 mill. tons mushrooms
0.93 mill. tons green peas
0.77 mill. tons green beans
0.13 mill. tons chickpeas
3.9 mill. tons green coffee beans
(imported)
Fungi
Green peas
PROLIFIC at Luxembourg Creative!17 November 2020

22. PROLIFIC idea
PROLIFIC at Science is Wonderful!22
Integrated, flexible and scalable cascade of PROcesses for the extraction of proteins and
bioactive molecules from Legumes, Fungi and Coffee agro-industrial side streams
22-24 September 2020

23. Role of partners
23
Biomass ‘upcycler’
Business ‘broker’ / accelerators
Product developers & end-usersBiomass provider >>> >>>
PROLIFIC at Luxembourg Creative!17 November 2020

24. Product prototypes in different sectoral
applications
24
Food
8 product prototypes of
enhanced nutritional quality
• High protein bread
• Various breakfast cereals
and snacks
• Protein enriched pasta
• Vegan meat-surrogate
• Sausages enriched in
soluble fibres
• Hamburger with polyphenols
Feed
2 product prototypes
(functional/nutritional)
for
• pig
• poultry
Packaging
4 product prototypes of
bioactive or biodegra-
dable packaging
• Chitosan based coating
for dry-cured meat
• Biodegradable and
bioactive packaging
• flexible (for meat)
• rigid (for cosmetic)
• coffee capsules
Cosmetics
2 product prototypes
• creams (anti-age,
whitening,
sunscreen)
• Toothpaste (anti-
microbial)
At least 16 product prototypes will be produced and validated at industrial level
PROLIFIC at Luxembourg Creative!17 November 2020

25. PROLIFIC powerpoint template25
What is the main contribution of
CELABOR in PROLIFIC Project?

26. Contribution of CELABOR in PROLIFIC
26
WP1
Inventory and
characterization of
the initial
feedstocks
WP2
Flexible and scalable
protocols for feedstock
pre-treatment and
extraction of the
fractions of interest WP3
Conversion of
fractions and
first steps
of extracts
valorisation
WP4
Evaluation and
selection of the
best performing
isolated
fractions
WP5
Industrial scale-
up of processes
and final product
prototyping
WP6
Life-Cycle
Assess-
ment (LCA)
and Cost-
Effective-
ness
Analysis
(CEA)
WP8 Project Coordination & Management
WP7 Dissemination, Exploitation & Commercialisation
PROLIFIC at Luxembourg Creative!17 November 2020

27. WP 2 – Flexible and scalable protocols for
feedstock pre-treatment and extraction of
the fractions of interest

28. Proteins
WP2 structure
28
Task 2.1: Pre-treatment, protein extraction,
fractionation and characterization (M3-18)
Subtask
2.1.1.
• Pre-treatment of the raw material
Subtask
2.1.2
• Protein extraction
Subtask
2.1.3
• General and detailed molecular
composition of the protein fractions
PolysaccharidesOther added-value compounds
polyphenols phytosterols carotenoidscaffeine
Task 2.2. Recovery of valuable non protein
compounds from residual solid fibre-rich fractions
(M9-21)
Subtask
2.2.1
• Polysaccharide fractions
Subtask
2.2.2
• Extraction of other added-value
compounds
Subtask
2.2.3
• Final residue analysis and
valorisation
PROLIFIC at Luxembourg Creative!17 November 2020
Chitin
Chitosan

29. 29
Cascade approach of coffee samples at CELABOR
SWE
alkali
extraction
Fibres
Residues
Ultrasound
assisted
extraction
Subcritical
water
extraction
Coffee
Silverskin
Coffee Green
Beans
Proteins
Slurry
Supercritical
CO2
extraction
Caffeine &
Phytosterols
Polyphenols
Residues
Supercritical
CO2
extraction
Caffeine &
Phytosterols
PROLIFIC at Luxembourg Creative!17 November 2020

30. 30
Cascade approach of legume samples at CELABOR
Classical
alkali
extraction
Fibres
Residues
Ultrasound
assisted
extraction
Proteins
Slurry
Supercritical
CO2
extraction
Carotenoids/
Phytosterols
Polyphenols
Residues
Supercritical
CO2
extraction
PROLIFIC at Luxembourg Creative!17 November 2020
Chickpeas Peas
EFAE**Environmentally friendly
aqueous extraction!
Green
beans
Carotenoids/
Phytosterols

31. Extraction of non-polar compounds
PROLIFIC at Luxembourg Creative!31 17 November 2020
Legume samples Coffee samples
Phytosterols Carotenoides Phytosterols Caffeine
▪ Campesterol
▪ Stigmasterol
▪ β-sitosterol
▪ Lutein
▪ Zeaxanthin
▪ β-cryptoxanthin
▪ Campesterol
▪ Stigmasterol
▪ β-sitosterol
Supercritical CO2 Extraction (SFE - CO2)

32. Extraction of polar compounds
PROLIFIC at Luxembourg Creative!32 17 November 2020
Subcritical Water Extraction (SWE)
Coffee samples
Starting material
▪ Coffee green beans (CGB)
▪ defatted coffee green beans
▪ dCGB slurryUAE
▪ Coffee silver skin (CSS)
Ultrasound Assisted Extraction (UAE)
Legumes and fungi
Starting material
▪ Chickpeas
▪ Peas
▪ Chickpeas slurryEFEA
▪ Peas slurryEFEA
▪ Agaricus bisporus (FABw)
▪ Pleurotus ostreatus (FPOhk)
▪ Lentinus edodes (FLEw)

33. LC-MS/MS Polyphenols identification
PROLIFIC at Luxembourg Creative!33 17 November 2020
25 standards
Flavonoids: myricetin, apigenin, naringenin, luteolin,
kaemferol, epicatechin, catechin, quercetin,
avicularin, quercitrin, isoquercitrin, hyperoside,
naringin, rutin.
Phenolic acids: hydroxybenzoic acid, protocatechuic
acid, coumaric acid, vanillic acid, gallic acid, caffeic
acid, ferulic acid, sinapic acid, neochlorogenic acid,
cryptochlorogenic acid, chlorogenic acid.
UPLC – Xevo TQS MS System (Waters)

34. Extraction of fibres
PROLIFIC at Luxembourg Creative!34 17 November 2020
Alkali SWE (Coffee) Hot alkali extraction (Fungi)
Cellulose/Hemicellulose
Chitosan
FLEw
FPOhk
Good filming properties

35. 35
Initial work on higher scale – Coffee samples
(up to 1kg/batch)
Supercritical CO2 Extraction (SFE - CO2)
Residues
Subcritical Water Extraction (SWE)
Caffeine
PolyphenolsFibres Residues
dCGBCGB
(+) bleaching (-) bleaching
CSS
PROLIFIC at Luxembourg Creative!17 November 2020
CSS
High Recovery of
Polyphenols & Fibres

36. WP 4 – Evaluation and selection of the best
performing isolated fractions according to
foreseen applications

37. Safety compliance of the extractsTask 4.1
M13 - M34
Fraction selection for foodTask 4.2
M13 - M36
Fraction selection for cosmeticsTask 4.3
M13 - M36
Selection of formulations of polymers and
composites for packagingM24 - M46
Fraction selection for feed
M13 - M36
Task 4.4
Task 4.5
WP4 - TASKS
37 PROLIFIC at Luxembourg Creative!17 November 2020

38. Desired fraction and product properties
38
Food
Hypo-allergic
High nutritional
Techno-functional
Antioxidant
Antibrowning
Antifungal & antimite
Feed
Anti-inflammatory
Agglutination
Antioxidant
Antimicrobial
Nutritional
Packaging
Antioxidant
Antifungal & antimite
Oxygen barrier
Thermal and
mechanical properties
Migration of additives
Biodegradability
Cosmetics
Antimicrobial
Antioxidant
Antiaging
Whitening
Water resist
Emulsifying
PROLIFIC at Luxembourg Creative!17 November 2020

39. On-going biological activities (CELABOR)
PROLIFIC at Luxembourg Creative!39 17 November 2020
❖ Anti-ageing activities : Anti-elastase & Anti-collegenase
❖ Anti-whitening activities : Anti-tyrosinase
❖ Antioxidant activities : FRAP & DPPH
❖ Anti-microbial activities

40. Main results of the project
PROLIFIC at Luxembourg Creative!40 17 November 2020
New Methodologies
Flexible, integrated and fully industrially scalable
Valuable compounds
proteins, peptides, amino acids, polyphenols, fibres
5 value chains
Completely new and bio-based!

41. High-protein bread
Some truly PROLIFIC products
41
Coffee capsules containing coffee
silver skin
Protein enriched extrudates for meat
surrogate foods
Legume proteins
PROLIFIC at Luxembourg Creative!17 November 2020

42. Follow us!

43. © CELABOR SCRL 43 43
Thank you for your attention!
CELABOR scrl
Research and Testing Centre in Food, Packaging, Environmental and Textile Technologies.
https://www.linkedin.com/company/celabor-scrl/
Website: www.celabor.be
Tel. : +32 (0)87 32 24 54
Dr. Job Tchoumtchoua
R&D Project Leader
jtc@celabor.be