This document describes a targeting plan to generate a hematopoietic stem progenitor cell specific reporter in human induced pluripotent stem cells (iPSCs) using CRISPR/Cas9 genome editing. The plan involves designing gRNAs to target an endogenous coding region. A targeting construct containing homologous arms and a T2A-H2B-mKO2 cassette will be used for homology directed repair. The gRNAs and targeting construct will be transfected into iPSCs and successful homologous recombination can be detected using digital droplet PCR.