1. Male Sterility
Rommel M. Garrido Jr., MSc
Department of Plant Breeding and Genetics
College of Agriculture and Food Sciences
Visayas State University
PBre112 – Methods in Plant Breeding
2. Autogamy
Mechanism that promote autogamy
1. Cleistogamy
2. Chasmogamy
Mechanism that prevent autogamy
1. Self-incompatibility
2. Male sterility
3. Dichogamy
4. Male Sterility
- a plant condition whereby pollens or anthers are non-functional
- this condition enforces cross pollination
- a tool used in hybrid seed production
Different manifestations:
- absence or scarcity of viable pollen
- absence or malformation of stamens or flowers
- failure of pollen to dehisce
5. Male Sterility
Kinds of male sterility:
a. True male sterility – plant lacks male sex organs or with abnormal
microspores
b. Functional sterility – plant lacks the ability to release pollen (even if
fertile)
c. Induced male sterility – sterility is induced with the use of physical or
chemical agents
6. True Male Sterility
Three kinds:
a. Genetic male sterility (GMS)
b. Cytoplasmic male sterility (CMS)
c. Cytoplasmic-genetic male sterility
7. Genetic Male Sterility
also known as nuclear or genic male sterility
manifested as pollen abortion or abnormal anther development
conditioned by a single recessive nuclear gene, ms
plants with Ms gene have normal anther development
8. Genetic Male Sterility
the sterility gene expression may vary with the environment
nearly all diploid and polyploid species of plants could have at least one
male sterility locus
the ms gene has been found in the ff. species:
- barley, cotton, soybean, tomato, potato, and lima bean
9. Genetic Male Sterility
how to identify genetic male sterility in plants?
Markers linked to GMS
- bright green hypocotyls in broccoli, potato leaf shape, and
green stem in tomato
Use molecular markers associated with GMS
- codominant markers are the best
10. Genetic Male Sterility
how to maintain the genetic male sterile types?
msms
X
Msms
msms
Msms
50% of the plants
will be male sterile
50% of the plants
will be male fertile
12. Cytoplasmic Male Sterility
controlled by the cytoplasm (mitochondrial gene)
- normal (N) cytoplasm are fertile
- sterile (s) cytoplasm causes male sterility or said to have CMS
CMS is maternally transmitted
has been found in the ff. species:
- corn, sorghum, sugar beet, carrot, sunflower, and flax
14. Cytoplasmic-Genetic Male Sterility
CMS are modified by the fertility-
restoring gene in the nucleus
Normal pollen or CMS is
ineffective when the Rf gene is
present
Three systems of CGMS
16. Summary
Male sterility is a tool to eliminate tedious and time-consuming
emasculation process in hybrid seed production
Use male sterile line as female parents
GMS is problematic (difficult to produce pure populations of male
sterile plants)
CMS is routinely used in hybrid seed production
Notas do Editor
the male sterility system should be stable in a wide range of environments and inhibit virtually all seed production