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Biotechnology
1.
Biotechnology A process
that uses bacteria or other microorganisms to create a product for industry
2.
3.
Blunt end cuts
A T T C G T A C T T T C G T A T A A G C A T G A A A G C A T
4.
Blunt end cuts
VIRUS!!
5.
Sticky end cuts
A T T G A A T T C T T G G C T T A A C T T A A G A A C C G A
6.
Action of Restriction
Enzyme VIRUS !
7.
Close-up of infected
DNA What if this section of DNA is viral DNA instead of bacterial?
8.
9.
How industry uses
restriction enzymes GENE X Insert Gene X into bacteria Use bacteria to make copies of Gene X
10.
How to isolate
“gene of interest” GENE Restriction enzyme site Restriction enzyme site
11.
Cutting the gene
out tube of restriction enzyme tube of DNA
12.
Preparing bacteria PLASMID
Cut the bacteria with the same restriction enzyme used to cut out the gene of interest
13.
“ Subcloning” gene
of interest GENE
14.
Cloning gene of
interest Allow bacteria to grow
15.
16.
Pouring gel
17.
Loading gel
18.
Setting voltage gradient
19.
Isolating gene Each
band represents DNA pieces of different sizes. One of these bands should have the gene of interest.
20.
Sample gel of
Salmonella
21.
Paternity test
22.
Sickle cell anemia
23.
24.
In what ways
can this technology help or hurt man?
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