ÄKTA is a diverse tool used to perform the purification of biomolecules in a faster and more reliable manner. It works on the principle of the UNICORN system.

1. ÄKTA PROTEIN PURIFICATION
Home Assignment- I
Submitted To:
Poornima Pandey Ma’am
Submitted by:
Bhagyashree Srivastava
LSMTT21019
2151096

2. PROTEIN PURIFICATION
 Isolation of one or a few proteins from a
complex mixture
 Characterization of the function, structure and
interactions of the protein of interest.

3. ÄKTA PROTEIN PURIFICATION SYSTEM
 Product from Cytiva- therapeutic manufacturer
 Purification of biomolecules: Chromatography
 Improved Speed, Performance and Flexible

4. UNICORN SYSTEM
 Gives interactive control
 Simplicity for quick evaluation of results
 Saves time with integrated DoE tool
Simplicity Power UNICORNTM
System

5. INSTRUMENTATION

7. ÄKTA START – AFFORDABLE AND EASY-TO-
USE PROTEIN PURIFICATION
 Automated Chromatography Technique
 Allows you to automate manual protein purification
procedure
 Gain deeper insights
 Simplifies work

8. ÄKTA GO - MAKE THE MOST OF VALUABLE
BENCH/COLD ROOM SPACE.
 Liquid Chromatography System
 Routine protein purification fitted into a footprint of
only 335 × 464 mm
 Intuitive method creation in minutes and interactive
process picture
 Proven design of AKTA systems and UNICORN
software

9. ÄKTA PURE – EASILY CUSTOMIZABLE TO MEET
YOUR PURIFICATION CHALLENGES
 Purification system for proteins, peptides, and
nucleic acids from microgram
 Tailor your protein purification system
 Intuitive software lets you focus on your research
 Match most purification challenges

10. ÄKTA AVANT – GAIN PRODUCTIVITY WITH FAST
AND SECURE DEVELOPMENT OF PURIFICATION
PROCESSES
 ÄKTA avant 25, designed for screening of
chromatography media and process optimization
 ÄKTA avant 150, suitable for scaling up to larger
columns.
 Increase efficiency in method development
 Reduce runtimes
 Minimize failures and maximize instrument use

11. BENEFITS OF ÄKTA SYSTEM
 Flexibility
 Alternatives methods focusing on flexibility
 ease of use
 Reliability
 Productivity
 ÄKTA avant allows process development and optimized
for chromatography
 Media screening and method optimization
 Scaling up to larger columns
 robust testing of the optimized process.

12. APPLICATIONS OF ÄKTA SYSTEM
 Protein Purification
 Chromatography
 Process Optimization
 Process Development
 Peptide Purification
 Preparative Chromatography
 HPLC
 Nucleic Acid Purification