Biosafety level 2 provides guidelines for working safely with agents that pose moderate hazards. It involves standard practices like prohibiting eating or drinking in labs, as well as special practices like using biohazard signs and restricting access. Personal protective equipment is also required, such as lab coats and gloves. Laboratory facilities at BSL-2 require features like lockable doors, hand washing sinks, and controlled ventilation to contain hazards and protect researchers.
3. INTRODUCTION
What is biosafety?
It is a system for the safe handling of toxic and
dangerous biological and chemical substances.
In medicine
It refers to the levels of lab containment protocols
It measured as biosafety level(BSL) 1,2,3,4 in
rising order of danger.
4. BSL- 2
BSL-2 builds upon BSL-1
It includes microbes that are
associated with human disease
But rarely serious.
Example :salmonella, HIV Virus
Staphylococcus aureus
5. Routes of Infection
Through the mouth
Eating, drinking and smoking in the laboratory
Mouth pipetting
Transfer of microorganisms to mouth by
contaminated fingers or articles
6. Continue…
Through the skin
Accidental inoculation with a hypodermic needle,
other sharp instrument or glass
Through the eye
Transfer of microorganisms to eyes by
contaminated fingers
Through the lungs
Inhalation of airborne microorganisms
8. Continued…
Not permitted in laboratories:
Eating
Drinking
Smoking
Pipetting by mouth
Handling contact lenses
9. Continued…
Never
Bend or break the needles
Discard needles or sharps into biological
waste bags
Discard needles into regular trash
10. Continued…
Minimize the creation of splashes or aerosols
Decontamination of work surfaces
Proper decontamination methods used
Materials to be decontaminated outside of
laboratory:
in a durable, leak proof container
closed container, easy to transport
11. SPECIAL PRACTICES
“Restricted Access
Restricted Access at BSL2 means
Persons who are at increased risk of acquiring infection, or
for whom infection may have serious consequences, are
not allowed in the laboratory or animal rooms
There are entry requirements for
workers in the lab
Doors are kept closed during
experiments
12. Biohazard sign
A biohazard sign must be posted on the entrance to
the laboratory
Appropriate information to be posted includes
• the agent(s) in use,
•the biosafety level,
•the required immunizations,
•the investigator's name and telephone number,
•any personal protective equipment that
must be worn in the laboratory
13. Cont…
All equipment - hoods, freezers, incubators,
centrifuges, etc. that are used with biohazardous
materials in a BSL-2 lab must have a biohazard label.
15. Cont …
high degree of precaution with any contaminated sharp
items, including needles and syringes, slides, pipettes,
capillary tubes, and scalpels
16. Needles & Sharps Precautions
DON’T place needles or sharps in waste containers
18. SPECIAL PRACTICES (Cont ..)
Laboratory personnel receive appropriate
immunizations or tests for the agents handled
Training with annual updates
Biosafety manual specific to lab
Decontaminate work surfaces
Report spills and accidents
No animals in laboratories