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Semelhante a ORDER of DRAW & TYPES OF BLOOD VIALS.pptx
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ORDER of DRAW & TYPES OF BLOOD VIALS.pptx
- 1. Dr. Bandana Singh MD Biochemistry Ex-Resident – AIIMS/VMMC/HRH ORDER OF DRAW
- 2. One of the most common nursing procedures is blood collection. Indeed, this is a technique practiced in all departments that allows investigation of the patient’s blood values, but it is not without risks. Nowadays the order of blood draw established by the international guidelines is postulated to avoid cross-contamination between different additives contained in blood collection tubes, and it is as follows: blood culture bottle, non-additive tube, coagulation tube, clot activator, serum separator tube, sodium heparin, plasma separator tube, ethylenediaminetetraacetic acid (EDTA), blood tube, and oxalate/fluoride.
- 3. Blood Culture Bottle (sterile) • 1st to be taken. • Aerobic • Anaerobic Maintain Sterility Next …
- 4. Blue Vial (Citrate Tube) • Trisodium citrate in liquid form. • Binds to calcium, • For Prothrombin time estimation- 1part of citrate and 9 part of blood. (1:9). • 200 microliter of citrate & 1.8 ml of blood. • For ESR estimation by Westergren technique. (@1:4). Next …
- 5. Red Vial (plain vial) • Doesn’t contain any Anticoagulant. • May contain clot activator like kaolin. • once clot form, serum separates, • CSF sample should not be collected in clot activator vial. • Don’t refrigerate the blood sample. Next …
- 6. Yellow Vial (Gel Tube) • Serum separation tube or Plasma separation tube. • Gel forms a physical barrier between serum/plasma & blood cells during centrifugation. Next …
- 7. Green Vial (Heparin Tube) • The walls of the tube are coated with either lithium heparin or sodium heparin. • Used for Karyotyping. Next …
- 8. EDTA Vial (Lavender) • EDTA is Ethylene diamine tetra acetate- chelates calcium. • Hemogram- RBC , WBC, PLT count, • HbA1C estimation, • Haemoglobin electrophoresis, • DNA & RNA isolation. Next …
- 9. PINK VIAL • Works in the same way as Lavender Vial, • This tube also contain EDTA as anticoagulation, • This vial is used in the Blood bank for Grouping and crossmatch. • Direct coomb’s test for autoimmune haemolytic anaemia. Next …
- 10. Grey Vial (Glucose Vial) • Sodium fluoride & potassium oxalate are used, • Fluoride inhibits Glycolytic enzyme Enolase thus decrease the consumption of glucose by the cells in the blood, Samples of Blood glucose estimation should be processed ASAP.