LINE OF DEFENCE IMMUNE CELLS MAST CELLS NEUTROPHILS NET’s ANTIGEN PRESENTING CELLS OSTEOIMMUNOLOGY PROSTAGLANDINS MATRIX METALLOPROTEINS

1. ROLE OF IMMUNE CELLS IN
HOST MICROBIAL INTERACTION
PREPARED BY,
DR. BEENA VIJAYAN PARVATHY

2. Jens
Martensson
CONTENTS
• LINE OF DEFENCE
• IMMUNE CELLS
• MAST CELLS
• NEUTROPHILS
• NET’s
• ANTIGEN PRESENTING CELLS
• OSTEOIMMUNOLOGY
• PROSTAGLANDINS
• MATRIX METALLOPROTEINS

3. Jens
Martensson
IMMUNITY
Resistance exhibited by the host towards injury caused by microorganisms and their
products.
IMMUNITY SYSTEM
Collection of cells, tissues and molecules that function to defend against infectious
microbes.

4. Jens
Martensson
LINE OF DEFENSE

5. Jens
Martensson
COMPONENETS OF IMMUNITY SYSTEM
• Cell-Mediated Immunity
• Antibody-Mediated/ Humoral Immunity

6. Jens
Martensson
IMMUNE CELLS

7. Jens
Martensson
MAST CELLS
• Resident leukocytes.
• Receptors:
 Complement system components -
C3a & C5a
 Fc portion of antibody molecules –
IgE : FcER & IgG : Fc Γr
 TLR’s
• Contains lysosomes.
• Triggered by:
 IgE &IgG antibodies
 Antigens
 Cytokines
 C3a & C5a
 Drugs, Physical activity, Hormones

8. Jens
Martensson
NEUTROPHILS
• Polymorphonuclear leukocytes
• Crucial role in host defense.
• Contains 3 functional types of granules
 Azurophilic/Primary granules
Function: Phagocytosis
 Specific/Secondary granules
Named as “adhesomes”
 Gelatinase containing/Secretory/Tertiary granules
Function: Extravasation process

9. Jens
Martensson
Regulation of Neutrophil Production &
Distribution
Production Trafficking Clearance
• After entering circulation
• Transendothelial migration
• Chemotaxis
• Opsonization
• Phagocytosis “Abortive phagocytosis”
• Killing & Digestion

10. Jens
Martensson
TRANSENDOTHELIAL MIGRATION

11. Jens
Martensson
CHEMOTAXIS

12. Jens
Martensson
PHAGOCYTOSIS

13. Jens
Martensson
KILLING AND DIGESTION

14. Jens
Martensson
NEUTROPHIL EXTRACELLULAR TRAPS (NET's)
(Brinkmann et al, 2004)
PMN’s Viable  VITAL NETosis (Yipp B G, 2013)
NET’s after nuclear membrane & granular digestion or cell death  SUICIDAL NETosis
(Brinkmann et al, 2007,2018)

15. Jens
Martensson
ANTIGEN PRESENTING CELLS
Dendritic Cells
B Cells
Macrophages/Monocytes

16. Jens
Martensson
MACROPHAGES

17. Jens
Martensson
1. Adhesion
2. Scanning
3. Co stimulation
4. Differentiation
EVENTS IN T CELLACTIVATION

18. Jens
Martensson
PHASES OF IMMUNE REPONSE

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Martensson
19
OSTEOIMMUNOLOGY

20. Jens
Martensson
PROSTOGLANDINS

21. Jens
Martensson
MATRIX METALLOPROTEINS