Drug Target Identification

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Arvind306

Saúde e medicina Tecnologia

Drug Target Identification for Hepatitis - B Guided by: Presented By : Arvind Singh Kamal Sharma MSc. Biotechnology

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For many years, pharmaceutical research companies have developed new drugs using a standard drug discovery process. The process usually begins with extensive medical research about a particular disease, which provides researchers with a better understanding of the disease and how it affects the body. The next step of the drug discovery process typically involves target identification and target validation.

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Microarrays Target identification seeks to identify new targets, normally proteins (or DNA/RNA), whose modulation might inhibit or reverse disease progression. Current technologies enable researchers to attempt to correlate changes in gene (genomics) and protein (proteomics) expression with human disease, in the hope of finding new targets. Microarrays are a well-utilized tool in both academic and industrial research laboratories. They can be used to assess gene and protein expression (via nucleic acid or protein microarrays) to identify novel targets, and can also be used to validate the found targets at the tissue or cell scale (via tissue or cell microarrays

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The Biology of HIV/AIDS Acquired immune deficiency syndrome (AIDS) is a disease characterized by the progressive deterioration of an individual's immune system. The immunological impairment allows infectious agents such as viruses, bacteria, fungi and parasites to invade the body and propagate unchecked. In addition, the incidence of certain cancers dramatically increases in these patients because of faulty immunosurveillance. AIDS is a serious threat to human health and is a global problem. Intensive research is being done to advance methods of detection, clinical treatment and prevention. The HIV Virus The AIDS etiologic agent is the human immunodeficiency virus type 1 (HIV-1), a retrovirus. HIV-1 contains an RNA genome and the RNA-dependent-DNA-polymerase termed reverse transcriptase. Members of the retrovirus family are involved in the pathogenesis of certain types of leukemias and other sarcomas in humans and animals. The structure and replication mechanism of HIV is very similar to other retroviruses. However, HIV is unique in some of its properties - it specifically targets the immune system, is very immunoevasive, forms significant amounts of progeny virus in vivo during initial stages of infection and can be transmitted during sexual activity. The HIV viral particle is surrounded by a lipid Human Immunodeficiency Virus bilayer derived from the host cell membrane during budding. The viral proteins are identified by the prefix gp (glycoprotein) or p (protein) followed by a number indicating the approximate molecular weight in kilodaltons. The lipid bilayer contains gp120 and gp41. These two proteins are proteolytic products of the precursor gp160. The gp41 anchors gp120 in the bilayer. The protein gp120 is routinely used as a diagnostic marker for HIV in Western Blot Analysis. More recently other viral gp proteins are also included in the test. Beneath the bilayer is a capsid consisting of p17 and p18. Within this shell is the viral core. The walls of the core consist of p 24 and p25. Within the core are two identical RNA molecules, 9800 nucleotides in length. Hydrogen bonded to each viral RNA is a cellular tRNA molecule. The viral RNA is coated by tightly bound molecules of p 7 and p 9 . The core also contains approximately 50 molecules of reverse transcriptase. There are several other viral proteins whose precise functions are not fully understood. The virus can be grown in tissue culture for diagnostic and research purposes. Several of the viral proteins have been cloned and generated in relatively large quantities. An individual can receive an inoculum of HIV through an abrasion in a mucosal surface (e.g., genital and rectal walls), a blood transfusion, or by intravenous injection with a contaminated needle. Virus or virally infected cells are found in body fluids such as semen and blood. The most important target for the virus is hematopoietic cells such as bone marrow derived monocytes, myelocytes and lymphocytes. Infection of im.

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