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Similar a Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia (20)


Mechanism of PDZK1 in Hepatocellular Carcinoma Complicated with Hyperuricemia

  1. By: Alejandro Mazo Ramírez Universidad Pontificia Bolivariana Facultad de Medicina. III semestre
  2. Introduction • Also known as hepatoma, its the most common type of primary liver cancer. It is often produced in people who have long-term diseases or bad habits, such as hepatitis virus type B or type C, being a long time smoker, heavy drinking or having cirrhosis. Hepatocellular Carcinoma
  3. Hyperuricemia Introduction • Uric acid is the final product of the purine catabolism. When its levels in blood are high enough, aside from acidifying it, it may denote other metabolic syndromes. • Previous studies have proved that the serum uric acid level can increase the incidence rate and mortality of malignant tumors.
  4. Objective • Finding the mechanism of PDZK1 in hepatocellular carcinoma complicated with hyperuricemia
  5. Methods Flow Cytometry Assay RT-PCR Analysis In this study, single cells are rapidly analized as they pass through multiple lasers in a solution It can determine characteristics of certain cells or even identify tumor markers It is done to see if a certain genetic sequence is present when the study is done. Here they are evaluating the expression of a protein (PDZK1)
  6. Methods Western blot assay In Vivo Tumorigenesis A sample of HCC was taken, and after some processing it was transplanted into mices with hiperuricemia. After sacrificing them a sample of tumor tissue was taken. This technique is used to detect a specific protein in a sample (blood or tissue are commonly used) It is used gel electrophoresis to separate proteins according to their molecular weight.
  7. Results
  8. Results
  9. Discussion Author What they said Agree/Disagree M. Chen, X. Lu, C. Lu et al [35] In our study, the mRNA expression of PDZK1 was signifcantly increased after HCC after diferent concentrations of soluble uric acid treatment Agree I. Andia and E. Rubio- Azpeitia [36] On the other hand, the interaction between urate crystals and immune cells can enhance immunosuppression and promote angiogenesis Agree S. Vadakedath and V. Kandi[38–40] Regulatory PDZK1 expression can afect the proliferation, migration, and apoptosis of HCC; uric acid may cause tumor immune response. It suggests that PDZK1 may be related to tumor immunity Agree
  10. • With the use of molecular biology new interactions between different diseases are being found, resulting in new treatments never thought to be found before Conclusions • The understanding of the different impacts that proteins have in the organism allow the emergence of new approachs to various diseases