The EasyGene Oligo Factory is a turn-key system that integrates oligonucleotide synthesis and post-synthesis processing within a single cabinet. It allows for the synthesis of 24 oligos simultaneously, and subsequent cleavage, deprotection, purification, and drying to be performed on the synthesized oligos. The system reduces costs to €0.05 per base and provides high quality oligos with minimal hands-on time through its integrated and automated workflow. It offers a more sustainable solution through its low chemical usage and energy consumption.

1. EasyGene Oligo Factory
Developed by Peter
Jepsen
Åvej 25
DK-3500 Værløse
Ph: +45 42724343

2. Turn-key Oligo Factory
From synthesis to final oligos within a cabinet
 Synthesis of oligos
 Cleavage from the
support
 Removal of protection
groups
 Purification of the
oligos
 Dry down oligos

3. Synthesis of oligos
 20 bottle positions  The effective and
compact EasyGene
 Reaction rotor for apparatus defines a new
simultaneous synthesis of standard within oligo
24 oligos synthesis
 Snap-On bottle holders  The apparatus is
for quick bottle exchange intelligently constructed,
using a minimum of
materials and machine
 Push-On fittings for quick parts
maintenance and service
 Reduced tubing results
 Supports different bottle in low death volumes,
types accurate delivery of
chemicals and reduced
 Snap-On valves for quick chemical consumption
user friendly maintenance Two 24 oligo synthesizers
 The apparatus is
 Green chemistry, due to controlled by user
low consumption of friendly and flexible
materials and chemicals software

4. Cleavage from the support
 Push-On fittings for quick  The oligo is cleaved from
maintenance and service the solid phase synthesis
support directly from the
synthesis rotor into
 Supports different bottle standard vials
types
 During the cleavage
 Snap-On valves for quick process aliquots of
user friendly maintenance NH4OH are added to the
columns at specified
 Same spare parts as used time intervals and
in the synthesizer incubated at room
temperature. The
liberated oligo is eluted
into vials for further
processing.
24 column cleavage apparatus  The apparatus is
controlled by user
friendly and flexible
software

5. Removal of protection groups
 The oligo eluted in
NH4OH is incubated at
55oC for fex. 6 hours for
removal of protection
groups
Oven for deprotection

6. Purification of oligos
 Push-On fittings for quick  The 24 well rotor is
maintenance and service loaded with trityl-on
reversed-phase column
material
 Supports different bottle
types
 The deprotected oligos
are added to the wells
 Snap-On valves for quick and the oligos are
user friendly maintenance purified according to the
instructions of the
 Same spare parts as used manufacturer
in the synthesizer
 In the final step the
oligos are eluted into
standard vials for further
processing.
24 column purification  The purification process
is controlled by the
EasyGene software.

7. Dry down oligos
 To dry down the oligos
the elution buffer from
the purification process
is evaporated by using
heat and a dry air flow
 The oligos are now ready
for shipment
Two 24 column evaporators

8. Plug and Play
 Easy to service
 The user can easily
change the apparatus
 Same software for all
machines
 Custom design
available
Easy to service

9. High quality oligonucleotides
 HPLC analysis of a 25-
mer oligo produced on
the EasyGene
apparatus
 The analysis shows that
only full-length oligo
product is present in the
oligo preparation.
Truncated N-1 oligo
versions cannot be
observed

10. EasyGene - Low production expences
EasyGene 24 Column  Oligo production on in-house
Chemicals Price pr/ml ml/base Price/base
Acetronitrile € 0,024 0,25 € 0,0060 24-well rotor
Deblock € 0,035 0,15 € 0,0053
Activator € 0,170 0,05 € 0,0085
Amidit € 0,420 0,05 € 0,0210
Cap B € 0,080 0,035 € 0,0028
Cap A € 0,07 0,035 € 0,0026
Oxidizer € 0,07 0,05 € 0,0035
$/base (50 nmol scale) € 0,05
Chemical expenses for a 20 mer oligo € 0,99
Bulk CPG/oligo € 0,05
Total for a 20 mer oligo € 1,04
K&A Laborgeraete 8/16/32 Column  Oligo production on
Chemicals Price pr/ml ml/base Price/base commercial single oligo
Acetronitrile € 0,024 2 € 0,0480
Deblock € 0,035 0,4 € 0,0140
columns
Activator € 0,170 0,09 € 0,0153
Amidit € 0,420 0,05 € 0,0210
Cap B € 0,080 0,075 € 0,0060
Cap A € 0,07 0,075 € 0,0056
Oxidizer € 0,07 0,12 € 0,0084
$/base (50 nmol scale) € 0,12
Chemical expenses for a 20 mer oligo € 2,37
CPG Column/oligo € 1,50
Total for a 20 mer oligo $ 3,87

11. Oligo prices in Europe
From commercial oligo producers
Price/base in € Price/oligo in €

12. Oligo prices in US/Canada
From commercial oligo producers
Price/base in € Price/oligo in €

13. EasyGene Oligo Factory
 “First-Mover” The only solution on the market, offering synthesis and
post synthesis processing in one cabinet.
 The cost of an oligo is reduced to € 0.05 per base
 High Quality oligos
 Quick and easy to service
 Minimum hands-on time
 Cleantech /Green chemistry
• Low chemical consumption
• Low energy consumption
• Fewer spare parts