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Effect of Cosmetic Preservatives
on Urine Stability
Team Members: Marco Cienega, Libby Joachim, Sandhya Ramesh,
Ben Smith, Caitlin Riegler, Laura Bradt, and Alan Lee
Grad Student Mentor: Dr. Aditya Raut Desai
Faculty Mentor: Dr. Bryan Boulanger
NASA JSC Mentor: Niklas Adam
Space Engineering Research Center
Texas Engineering Experiment Station, Texas A&M University
Team Structure
Name Major Year Position
Libby Joachim University Studies Honors 4 Team Lead
Marco Cienega Mechanical Engineering 4 Safety Lead
Ben Smith Civil Engineering 3 Assistant Lab Lead
Sandhya Ramesh Biomedical Engineering 2 Logistics Lead
Caitlin Riegler Civil Engineering 2 Data Lead
Alan Lee Chemical Engineering 1 Lab Lead
Laura Bradt Chemical Engineering 1 Assistant Team Lead
Human Habitation Challenges
Not economical or practical to re‐supply
basic life support elements from Earth.
Source: NASA
Our Focus
Prevention of:
• Biofilm Formation
• Solids Precipitation
Current Urine Pretreatment Method
“String of Pearls”
Urine and fecal 
collection unit
•Oxone and sulfuric 
acid are toxic
•Bad for astronauts 
and water 
reclamation system
Project Goals
• Improve safety of pretreatment process
• Improve reclamation system
performance
Possible Solutions Investigated
• Eliminate need for chemical pretreatment
– Change collection protocols
– UV treatments
• Use non-toxic pretreatment agents
– Cosmetic preservatives
Semester Focus
Develop QAPP based on NASA
mentor’s recommendations and
screen cosmetic preservatives for
their ability to stabilize urine.
Experimental Design
Urine 
collected
Sampling
0 and 24 hrs
Seeding and Mixing 200 mL
Preservatives 
Added
Analytical Methods
Ion 
Chromatography
TurbiditypH
Chemical Oxygen 
Demand
Phenate
Total Organic 
Carbon
Preservatives Tested
Formaldehyde - F
Diazolidinyl Urea - DU
Quaternium15 - Q15
Bronopol - BRO
Sodium Hydroxymethyl Glycinate - SHG
Methyl Paraben - MP
Ethyl Paraben - EP
Phenoxyethanol - POE
Benzyl Alcohol - BA
Performance Comparison
Cosmetic Preservative
pH Turbidity NH3 TOC COD
(2‐4) (WRT SO) (≤1) (WRT SO) (WRT SO)
Formaldehyde (F)
Diazolidinyl Urea (DU)
Quaternium ‐ 15 (Q15)
Bronopol (BRO)
Sodium Hydroxymethyl Glycinate (SHG)
Methyl Paraben (MP)
Ethyl Paraben (EP)
Phenoxyethanol (POE)
Benzyl Alcohol (BA)
Parameters
Conclusions
• The most promising cosmetic preservatives:
– Formaldehyde
– Diazolidinyl Urea
– Sodium Hydroxymethyl Glycinate
– Bronopol
Future Work
• Further investigation of compatible
cosmetic preservatives:
– Lowest effective concentration.
• Computer modeling to determine chemical
recovery for reuse.
• Combining two or more chemicals to get
desired results.
References
• Eaton, A. D. et al. (2005). Standard Methods for the
Examination of Water and Wastewater, American
Public Health Association, Washington D.C.
• NASA (2005). “Standard Operating Procedure for
the Formulation of Batch U.S. Pretreated Urine
(2005 Revision Basic).” National Aeronautics and
Space Administration, Lyndon B. Johnson Space
Center, Houston, TX, EC3-WR-066.
Acknowledgements
Niklas Adam
Karen Pickering
Bryan Boulanger
Aditya Raut Desai
Magdalini Lagoudas
Cable Kurwitz
Pranav Nagarnaik
Questions?

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Pretreatment