1. GENE THERAPY IN THE TREATMENT
OF CANCER CELL
NETAJI SUBHAS CHANDRA BOSE
INSTITUTE OF PHARMACY

2. What Are Genes?
Genes are carried on
chromosomes and are the basic
physical and functional units of
heredity
Genes are specific sequences of
bases that encode instructions on
how to make proteins
When genes are altered so that the
encoded proteins are unable to
carry out their normal functions,
genetic disorders result

5. Diseases For Applying Gene Therapy
Disease Defect Target cell
Severe combined Bone marrow cells or
immunodeficiency T-lymphocytes
Hemophilia Liver, muscle
Cystic fibrosis Lung Cells
Cancer Many cell types
Neurological diseases Parkinson’s/ Alzheimer's Nerve Cells
Infectious diseases AIDS, hepatitis B White Blood Cells

6. P53 gene ADENOVIRUS
TWO COMPONENTS

7. ADENOVIRUS IS A TYPE OF VECTOR

8. (ADENOVIRUS)

9. HOW IS GENE THERAPY ADMINISTERED?
INJECTION INTO PLEURAL AND PERITONEAL CAVITIES DIRECT INJECTION TO TUMOR
INTRAVENOUS INFUSION

10. The adenovirus
containing the p53 tumor
suppressor gene binds to
the receptor in the cell
membrane of the cancer
cell
HOW DOES GENETHERAPY WORKS INTHE CANCER
CELL?

11. The adenovirus is
then packaged in a
vesicle
The vesicle proceeds
to the cell nucleus
The vesicle breaks
down, releasing the cell
nucleus
The adenovirus
injects its gene, which
contain the p53

12. The adenovirus
injects its gene, which
now includes the p53
tumor suppressor
gene, into the cell
nucleus
The cancer cell then
makes p53 protein

13. The p53
protein
causes the
cancer cell to
self destruct
without
affecting
surrounding
normal cell

14. WHATS HAPPEN AFTER THE TREATMENT?
LIVER CANCER
MALE, 60 y.o.
BEFORE
AFTER
ADVANCED LUNG CANCER
MALE, 62 y.o.

15. LUNG CANCER
MALE, 55 y.o.
CANCER OF THE LOWER LIP
MALE, 67 y.o.
BEFORE
AFTER