2. BIO CHEMISTRTY
• The element which carries the total amount of the purjucalation on
the elements of the regarding formulas which can be the powder
form of the following elements is that which always allow the carbon
and hydrogens of the following compounds because if there will be
any elements which found impure they will be regarding of the
functions and the other of the compounds are bringing the impurities
otherwise there will be none of the following, impurities will be begin
of the following compounds which can either be taken as forgiven or
the compound will damage.
3. MOLISCH TEST
Molisch test is a general test for carbohydrates. This test is useful in
identification of any compound that can be dehydrated to furfural or
hydroxymethylfurfural in the presence of conc H2SO4 Furfural is
derived from the dehydration off pentoses, while
hydroxymethylfurfural is produced from hescoses,
oligosacchidries & polysachidres are hydrolyzed to yield their
constituent monomers by the acid. The ALPHA napthol reacts
with cyclic aldehydes to form purple coloured condensation
products. A negative result indicates the absence of
carbohydrates. Molisch’s reagent is a solution of 10% ALPHA
napthol in 95% ethanol.
4. IODINE TEST
• Iodine test is useful to distinguish mono and disaccharides from
polysaccharides. They changes colour with iodine solution. The
amylose compound of starch has a helical structure when it is treated
with iodine solution. Iodine is trapped inside the coli like structure
and the complex has an intense blue colour. When the amylose
solution is heated, the helical conformation is disrupted & it loses its
capacity to bind with iodine. On cooling the original conformation is
regained & the capacity to bind iodine is also recovered. Any
lopection & glycogen because of the branched structure gives purple
or red colour with iodine..
5. LUGOL’S IODINE REAGENT
• Dissolve 10g potassium iodide in 100ml of distilled water. Slowly add
5g iodine crystal, while shaking. Filter & store tightly stoppered brown
bottle.
• The glycerin (
6. REDUCING SUGAR TEST
BENEDICT’S TEST(Standby Rossiter Benedict)
• Reducing sugars by virtue of its free aldehyde or ketone group in the
structure reduces cupric ions as in alkaline medium at high
temperature which is also be taken as the frequency of the solution
which can be measured by the ph paper or litmus paper or it can
resisted by the volume of analysis process or ceutics development
which can help in organic chemistry of the given substituents. It
develops the polycaps within the circumstance or with the benedict
beaker.
• Here by the test
7. Organic Compounds
• There is the compound which gives the elements to the symbolic
molecules iodine compound which reacts to sodium directly if
outermost layer – visceral pertionium
• Layer of serous fluid producing cells (mesothlium)
• Serousa <outermost layer-