cbsh bdp sir asjad raza asjad raza rajeev kumar cbsh pusa expression analysis primer nanodrop reading gel doc ethidium bromide agarose gel centrifugation extraction buffer β- mercaptoethanol pvp normalization of rna spectrophotometer gel electrophoresis ctab method wheat genotype quantification nucleic acid cdna synthesis dna isolation rna isolation principle and application plant genetic transformation spontaneous excision non‐recombinase r/rs flp/frt cre/loxp intra chromosomal homologous transposon‐based marker site specific recombinatio co-transformation marker free approach clean gene technology epigenetics regulation rna interference mrna silencing genome editing rna splicing terminal uridylyl transferase grna mrna srna molecular biology of gene mrna transport guided dna directed site specific deamination biotechnology 1905101013 gene regulation epigenetics rna editing
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