At the Blossom Fertility and IVF Centre Surat,Laser Assisted Hatching technique is used to perform the assisted hatching procedure. Doctors and embryologist are well conversant with the latest technology in the field of ART (Assisted Reproductive Technology). You can contact the top IVF doctors,Fertility specialist Surat and embryologist in surat at http://www.blossomivfindia.com or at http://www.blossomivfindia.com/fertility-treatments

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One of the most frustrating aspects of assisted reproductive technology for patients and
fertility professionals alike is having to deal with failure in fertility treatment. This is
especially true in couples who have attempted assisted reproductive procedures many times,
and also in those whose time is running out because of their age. Now, a recently developed
technique, assisted hatching, is offering new hope to the couples who fall into these
categories.
Assisted hatching is a laboratory technique used with IVF. It involves the use of laser to thin
the outer shell (zone pellucida) of the fertilized egg, before the embryo is transferred into the
uterus. Assisted hatching is used to enhance the embryos ability to hatch, and also implant,
after transfer. The procedure is based on the fact that an alteration in zona pellucida (outer
covering of egg) either by drilling a hole through it or by thinning it, will promote hatching or
implantation of embryos that are otherwise unable to escape intact from the zona pellucida.
After a fertilized egg is returned to the uterus, several things must happen:
1. It must continue to divide and grow (it is now called an embryo).
2. The embryo must break out of the zona pellucida (usually called the zona) which is a
hard protein shell that surrounds it.
3. The embryo must then burrow into the lining of the uterus (called the endometrium)
and continue to grow there.
It has been suggested that making a hole in or thinning this outer layer may help embryos to
‘hatch’, increasing the chances of the woman becoming pregnant in some cases. Assisted
hatching is indicated for infertile couples in following cases.
1. when the zona pellucida is noted to be excessively thick when measured on a day 3
embryo by the embryologist
2. with poor embryo quality
3. In women with an elevated Day 3 FSH

2. 4. when a Flare stimulation protocol has been used
5. when the female partner age is 38 or older at the time of stimulation, or
6. with previous IVF implantation failure of embryos to implant though results were
otherwise good
7. Frozen embryo replacements.
How does the procedure of Assisted Hatching work?
Assisted hatching is carried out in the laboratory by experienced embryologists. The assisted
hatching procedure, like ICSI, is carried out by a technique known as micromanipulation. In
small dishes the embryos, which now contain an average of six to eight cells, are stabilized
by a holding pipette, while on the opposite side a small pipette containing acidified Tyrode's
solution creates a small defect in the zona. Just before the embryos are replaced, whether they
are fresh or frozen/thawed, a small hole is made in the zona, or by thinning it using a
micromanipulation technique. The process is repeated for each embryo. The main methods
currently in use for assisted hatching are: chemical, mechanical and laser. The process will
damage about 1% of embryos.
Most IVF clinics will not perform assisted hatching if there is one embryo available because
of possible damage to this embryo would result in no embryo transfer. The assisted hatched
embryos are then transferred into the uterine cavity as usual. Some clinics will give the
woman a course of antibiotics to prevent infection. (Assisted hatching deprives the embryo of
its intact protective coat, which shields it from exposure to any harmful factors in the uterus).
Assisted Hatching using laser technology is probably the best technique. Laser assisted
hatching is a gentle and safe way to weaken a part of zona pellucida. Several studies have
shown that using a laser is superior to chemical and manual hatching. Minimal handling of
the embryo and delivering fast and exact control over the drilling of the hole are the
advantages of laser assisted hatching.
Advantages of using Assisted Hatching
This relatively small variation in the IVF procedure has yielded dramatic results in older
patients and those with previously failed cycles.
1) It provides “mechanical advantage” to the embryos in breaking the zona for implantation.
2) It also ensures early contact of embryos with endometrium. This expedites enzymetic
reaction between endometrium and embryos called “Embryo endometrium cross talk”.

3. 3) Mechanical advantages and chemical advantage ensure better implantation rate and better
pregnancy rate.
4) It helps women with previous failed IVF cycle.
Disadvantages
The addition of assisted hatching to the standard IVF protocol does add extra laboratory
manipulation and therefore added costs. There is a small risk of damage to the embryo during
the micromanipulation process or at the time of transfer, and there is a slight increase in
identical twinning. There is a greater chance of fetal complications and abnormalities in some
identical twins. A rare complication of identical twinning is conjoined or "Siamese" twins.
No higher rate of identical twins is observed than with routine IVF. This may relate to
whether a large enough opening is made in the zona to prevent pinching of the embryo during
the hatching process.
Assisted hatching of IVF embryos, is an Assisted Reproductive Technology procedure which
is performed in IVF laboratory. It increases IVF success rates. Consult infertility expert at
Blossom Fertility centre in Surat for complete medical advice for infertile couples.
At the Blossom Fertility and IVF Centre Surat, Laser Assisted Hatching technique is
used to perform the assisted hatching procedure.Doctors and embryologist are well
conversant with the latest technology in the field of ART (Assisted Reproductive
Technology). You can contact the top IVF doctors, Fertility specialist Surat and embryologist
in surat at Blossomivfindia.com or at IVFfertility-treatments
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