explanation of structure's western blot

1. Western Blot OR protein immunoblot N.Ghasemnejad M.S student Iran, shahid Beheshti Medical university , National Nutrition and Food Technology Research Institute WESTERN BLOT

7. WESTERN BLOT

10. WESTERN BLOT

13. Loading the SDS-PAGE gel WESTERN BLOT

16. WESTERN BLOT Proteins here are actually linearized .

21. WESTERN BLOT

31. Detection by Chemiluminescence

35. Creating Western Blot Strips WESTERN BLOT 1-HIV lysate proteins are separated by size using gel electrophoresis 2-Proteins are transferred (blotted) onto the surface of a membrane 4-Strips are incubated with patient serum and antihuman IgG conjugated with an enzyme (and chromagen) 3-The membrane is cut into strips

36. HIV Western Blot Banding Pattern WESTERN BLOT env gp160 gp120 gp 41 gag p55 p18 p24 pol p65 p51 p31

39. WESTERN BLOT