1. (RFLP)

2.  A difference in DNA sequence among individuals,
groups or populations.
• Genetic Mutation-: A change in the nucleotide
sequence of a DNA molecule.
 Polymorphism can lead to mutations.

3.  The human genome is 3x109 bp
 On average two individuals (humans) vary at 1 in 1000 bp
 This means that they will differ in more than 3 million bp
 ITS POSSIBLE because of recognition sites for Restriction
enzymes.
 “ variations in homologous DNA sequences”

4.  To determine this we must do southern blotting for
both specific sequenced DNA samples.
 After that we can comment the variations between two
samples.
 It also can be used for genome mapping
 We can also do this to determine the homozygous
,heterozygous type of two allels

6.  And DNA finger printing also possible with this
method.
 Most commonly used for law and order purposes.