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WEFTA 2008 38th WEFTA Annual Meeting  Florence, Italy. 17-19 September 2008 Simulated live shipment of edible  crab  Cancer pagurus : influence of  temperature, air exposure and  anesthetic on physiological  stress   Sara  Barrento Bárbara Teixeira António Marques Paulo Vaz-Pires  Maria Leonor Nunes  IPIMAR
Edible crab   live trade in Portugal ,[object Object],[object Object],National production is insufficient However...
Live trade conditions 10 tons crab Astrid K. Woll Unloaded and discarded manually (during 2-3 hours) Sara Barrento Transport in vivier trucks during  24 – 48 h Sara Barrento
Transportation conditions - 1Kg crab/ 1L water  -  no filtration  -  just aeration  -  refrigeration temperature =  13-15º C  Truck tanks (24 - 48 h): ,[object Object]
Overall, what happens during live trade?   ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Transportation condition alternatives ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Anesthetics  ,[object Object],[object Object],But ...  -  developed by New Zealand Institute of Crop and Food Research  -  active principle is isoeugenol (NZ$ 300 l-1; 3 cents/kg fish)  -  it is approved as a  zero withdrawl fin fish, crustaceans  and shellfish anaesthetic  in: New Zealand  Australia Faroe Islands  Chile either requires a  21 days post treatment   withdrawal  period before harvesting fish for food!
Aims ,[object Object],[object Object],[object Object],[object Object],in the physiological condition of crabs during simulated live shipment
Strategy ,[object Object],SEAWATER 1Kg/L No AQUI-S ® Simulate present conditions AQUI-S ® Decrease metabolism AIR EXPOSURE No AQUI-S ® AQUI-S ® Decrease costs Decrease costs and metabolism  8 ºC, 12 ºC and 16 ºC
Strategy ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],n = 95
Results Vigour and   Mortality 24 h 48 h 12 ºC 16 ºC 8 ºC
Results Vigour and   Mortality 12 º C 8 º C 16 º C ,[object Object],Air exposure and temperature  increases mortality 24 h T ºC not always increases mortality
Results Vigour and   Mortality ,[object Object],AQUI-S ®  and low temperatures might lower too much the metabolism ,[object Object],12 ºC 8 ºC 16 ºC 24 h
Results Glucose, proteins and hemocyanine ,[object Object],[object Object],[object Object],[object Object],[object Object]
Results Lactate concentration and pH WT WQS AIR AQS Lactate T(0h)= 10 mmol pH  T(0)=7.23  min (24h)  6.99  16 º C 16 º C max (24h)= 30   max (48h)= 70   no change 12 º C 12 º C no change no change no change no change   max (48h)= 80 min (48h) (48h)=7.03 max (48h)= 70 no change min (48h) ( 6.67 ) max (48h)= 90   min(24h) ( 6.99 ) max (24h)= 50   8 º C 8 º C no change  no changes no change no changes no change no changes no change no changes
Summary ,[object Object],Lactate  pH  ,[object Object],[object Object],[object Object],[object Object],But... Lactate  pH  Air exposure Seawater
Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Future perspectives ,[object Object],[object Object],[object Object]
Acknowledgements Collective Research Project  “CrustaSea: Development of best practice, grading and transportation technology in the crustacean fishery sector”,  funded by the EC
Hey, George, who told you this was a party??

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Simulated live shipment of edible crab Cancer pagurus: influence of temperature, air exposure and anesthetic on physiological stress.

  • 1. WEFTA 2008 38th WEFTA Annual Meeting Florence, Italy. 17-19 September 2008 Simulated live shipment of edible crab Cancer pagurus : influence of temperature, air exposure and anesthetic on physiological stress Sara Barrento Bárbara Teixeira António Marques Paulo Vaz-Pires Maria Leonor Nunes IPIMAR
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  • 3. Live trade conditions 10 tons crab Astrid K. Woll Unloaded and discarded manually (during 2-3 hours) Sara Barrento Transport in vivier trucks during 24 – 48 h Sara Barrento
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  • 11. Results Vigour and Mortality 24 h 48 h 12 ºC 16 ºC 8 ºC
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  • 15. Results Lactate concentration and pH WT WQS AIR AQS Lactate T(0h)= 10 mmol pH T(0)=7.23 min (24h) 6.99 16 º C 16 º C max (24h)= 30 max (48h)= 70 no change 12 º C 12 º C no change no change no change no change max (48h)= 80 min (48h) (48h)=7.03 max (48h)= 70 no change min (48h) ( 6.67 ) max (48h)= 90 min(24h) ( 6.99 ) max (24h)= 50 8 º C 8 º C no change no changes no change no changes no change no changes no change no changes
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  • 19. Acknowledgements Collective Research Project “CrustaSea: Development of best practice, grading and transportation technology in the crustacean fishery sector”, funded by the EC
  • 20. Hey, George, who told you this was a party??