1. Lecture # 1: Molecular and Cellular Biology Overview http:// www.youtube.com/watch?v =FZtRn1fst-g

2. WELCOME!

3. instructor Mark E Minie PhD [email_address]

4. Class mechanics…

5. The wiki… http:// uweobasicbiomolcelli.wikispaces.com /

7. A word about PMIDs and UW restricted resources…

8. Tonight’s timeline: 5:30 PM-6:30 PM: Shannon Kuyper Orientation 6:30 PM-6:45 PM: Break 6:45 PM-7:45 PM: Lecture # 1 7:45 PM-8:00 PM: General discussion and Q&A

10. What is life?: Dictionary.com Unabridged (v 1.1) - Cite This Source - Share This life laɪf/ Pronunciation Key - Show Spelled Pronunciation [lahyf] Pronunciation Key - Show IPA Pronunciation noun, plural lives laɪvz/ Pronunciation Key - Show Spelled Pronunciation [lahyvz] Pronunciation Key - Show IPA Pronunciation , adjective – noun 1.the condition that distinguishes organisms from inorganic objects and dead organisms, being manifested by growth through metabolism, reproduction, and the power of adaptation to environment through changes originating internally. 2.the sum of the distinguishing phenomena of organisms, esp. metabolism, growth, reproduction, and adaptation to environment.

17. Are aliens among us? PMID: 18237098

20. There are two different types of cells on Earth*… *Really three, but we won’t be talking about the Archaea today :) Prokaryote Eukaryote plant animal

21. And they are simultaneously the same but very different in structure, function, composition, behavior, etc...

22. Prokaryotic cell structure

23. Eukaryotic animal cell structure

24. Eukaryotic plant cell structure

25. Size-eukaryotes are generally bigger than prokaryotes

26. prokaryotes & eukaryotes have different genetic structures as well…

28. THE TREE OF LIFE Fun with genealogy. PMID: 9398070

31. Molecular motors http:// www.youtube.com/watch?v =InIha7bCTjM&feature=related

33. The central dogma of molecular biology

34. The sequences of these biomolecules are digital in nature… and thus lend themselves to digital analysis with computers… DNA Protein … arrsrarklqrmkql … … tggagatgacgtca … … 0111000101011111 … … 010110101110000 … RNA … uggagaugacguca … … 0111000101011111 …

36. http:// www.ncbi.nlm.nih.gov/sites/gquery A key bioinformatics resource—ENTREZ The National Center for Biotechnology Information (NCBI)

38. deoxyribonucleotide sequences self-organize into a double helix, amino acid sequences self-organize into various peptide structural motifs…

39. and it is this informational and self-organizing chemical nature that forms the basis for all structural and behavioral activities of cells…

40. and is the basis for our current concepts of the origin of cellular life on Earth…

41. “ Classic” Miller-Urey Experiment

43. Metabolome The sum total of the chemical reactions and pathways in a given organism

45. A “simple” metabolic pathway…GLYCOLYSIS http:// www.biocarta.com/pathfiles/h_glycolysisPathway.asp

46. http:// www.expasy.ch /tools/pathways/ Metabolic pathways and cellular processes THE BIG PICTURE

47. These cellular processes and metabolic pathways are mediated by proteins, carbohydrates, lipids, DNA, RNA and other molecular entities and function in “ real time”—they are only indirectly regulated and controlled by the cellular gene structure…

48. http:// www.biocyc.org / Computer generated models of metabolic pathways…

50. Vast amounts of data at every level, with the blurring barriers between biology and information technology DNA Sequence Data RNA Expression Data Protein Structure Data

51. http:// www.youtube.com/watch?v =5ukPD4G5eSw The inner complexity of a cell (eukaryote)…

52. Open discussion and Q&A