1. Patricia Linton Manchester Metropolitan University

2. It’s just so amazing – that’s what!

4. TRANSCRIPTION RNA PROCESSING DNA RNA transcript 3  5  RNA polymerase Poly-A Poly-A RNA transcript (pre-mRNA) Intron Exon NUCLEUS Aminoacyl-tRNA synthetase AMINO ACID ACTIVATION Amino acid tRNA CYTOPLASM Poly-A Growing polypeptide 3  Activated amino acid mRNA TRANSLATION Cap Ribosomal subunits Cap 5  E P A A Anticodon Ribosome Codon E The central dogma DNA RNA PROTEIN

5. TRANSCRIPTION TRANSLATION DNA mRNA Ribosome Polypeptide (a) Bacterial cell Nuclear envelope TRANSCRIPTION RNA PROCESSING Pre-mRNA DNA mRNA TRANSLATION Ribosome Polypeptide (b) Eukaryotic cell

7. (a) Tobacco plant expressing a firefly gene (b) Pig expressing a jellyfish gene

8. Kary Mullis – Genius or eccentric? ‘ Science grows like a weed every year’ ‘ Each of us have things and thoughts and descriptions of an amazing universe in our possession that kings in the 17th Century would have gone to war to possess’ ‘ Science consistently produces a new crop of miraculous truths and dazzling devices every year’ ‘ I have had an encounter with an extraterrestrial in the form of a fluorescent raccoon’ ??????? You can read his Nobel lecture here: http://nobelprize.org/nobel_prizes/chemistry/laureates/1993/mullis-lecture.html

9. PCR – in the dark ages 8 BORING hours per PCR! 95º C 5 min 35 times 55º C 3 min 72º C 5 min

13. The temperature profile of a PCR cycle is controlled by the thermal cycler program which results in a near exponential increase in PCR product accumulation for about the first 30 cycles. The 3 Os!!!!!

15. http://learn.genetics.utah.edu/content/labs/gel/

16. Mixture of DNA mol- ecules of different sizes Power source Longer molecules Shorter molecules Gel Anode Cathode 1 2 Power source – + + –

23. 7.6 DNA Fingerprinting Adult 1 Adult 2 Adult 3 Adult 4 Adult 5 Adult 6 Child 1 Child 2 Child 3

25. Making your own equipment