1. Texto adicional Texto adicional Texto adicional texto adicional Texto adicional texto adicional Lennis Bedoya Muñoz ID: 000087035 Juan Carlos Agudelo Jiménez ID:000149094 MOLECULAR BIOLOGY UPB Proteomic analysis of aqueous humor from patients with primary open angle glaucoma. Xiaoming Duan .

5. PRIMARY OPEN-ANGLE TYPE

7. CLOSED-ANGLE GLAUCOMA

14. PROTEOMICS HAS BEEN USED TO STUDY EYE DISEASES

16. Understand Proteomic analysis of aqueous humor from patients with primary open angle glaucoma to identify the possible roles of these AH proteins in POAG and the respective relationships between POAG and elevated intraocular pressure (IOP).

18. Estudiar proteínas diferenciales presentes en los pacientes con POAG. Distribución de las proteínas diferenciales presentes en el humor acuoso. Comprender de mejor manera la relación entre la biología molecular y el desarrollo de los pacientes con POAG

25. Concentración total de proteínas presentes en el humor acuoso

32. Patrones en el Gel de Electroforesis de 2 Dimensiones Paciente con Glaucoma Paciente Control

35. Identificación de Proteinas Prostaglandina Humana H2 D-Isomerasa Caspasa 14 transthyretin albumina transferrina albumina Cistatina C AQGFTEDTIVFLPQTDK

38. Doctors Argument Agreed Ferreira SM. “ Although POAG has been widely considered a multifactorial disease, IOP is the most important known risk factor for the development of glaucomatous optic nerve damage.” Grus FH, Joachim SC, Sandmann S, Thiel U, Bruns K, Lackner KJ, Pfeiffer N. “ Most recent insights regarding POAG have come from studies on animal models. Other studies of AH typically focus only on proteins. Grus et al. has studied transthyretin changes in AH of POAG using surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) ProteinChip arrays and two-dimensional electrophoresis.”

39. Author Argument Agreed Choi J “ Reducing IOP can still be considered the most beneficial treatment in terms of halting visual field damage progression, even in normal pressure glaucoma. However, the mechanisms of elevated IOP have not been fully elucidated. At present, it is well known that elevated IOP could result from a variety of cell deaths in the trabecular meshwork. AH, the circulated intraocular fluid, includes many kinds of cytokines, which could likely play significant roles in the trabecular meshwork.”

40. Doctors Argument Agreed Denecker G “ There were few reports about caspase 14 in eyes.Caspase-14 is a unique member of the evolutionarily conserved family of cysteinyl aspartate-specific proteinases, which are mainly involved in inflammation and apoptosis. Previous studies demonstrated that caspase 8 and caspase 9 are the main kinds of caspase that play roles in the apoptosis of ocular tissues. According to this study, caspase14 might be involved in the apoptosis of ocular tissues by either directly mediating or inducing caspase 8 and caspase 9 activation.”

45. Juan Carlos Agudelo