The slideshow I presented at the NNIN 2008 Convocation at Cornell. Look for video of the presentation at the NNIN website.

1. 2008 NNIN REU University of Michigan Flow Cytofluorometry with a Soft Elastomer-on-Silicon Nano Photonic Device for High-Speed Fluorescence Multi-Spectrum Acquisition Jose Guevarra Department of Mechanical Engineering University of California, Santa Barbara PI: Dr Katsuo Kurabayashi Mentor: Steven Truxal

2. What is Flow Cytofluorometry? Photo Detectors (Side Scatter) Data Acquisition Mirrors and Optics Photo Detector (Forward Scatter) Light Source HydroDynamic Focusing http://www.invitrogen.com/site/us/en/home/support/Tutorials.html Interrogation Zone Replace with Microfluidics Flow Cell Replace with MEMS Device A technique for sorting particles. Then analyzing them with fluorescence tagging

3. How is Fluorescence Used? Cell Fluorophore Tagged Antibody Excitation Light Emission Light 3. Collect Emission Light 1. Tag Cells with Fluorophores 2. Excite Cell Image: http://www.invitrogen.com/site/us/en/home/support/Tutorials.html

5. System Overview Microfluidics Chip (Focusing and Interrogation Zone) Measurements by Fluorescence Spectroscopy MEMS Spectroscopy device Excitation Light Emission Light

7. Use of Flexible Diffraction Grating Photo Detector Emission Light

9. Flow Cell Fabrication Silicon Substrate Su-8 10 Photoresist PDMS After Lithography Exposure and Develop

10. Flow Cell Fabrication PDMS Glass Punch Holes in Reservoirs Reservoirs MicroChannel Oxygen Plasma Bonding with Glass Remove Mold

11. Flow Cell In Action

13. Results – Fluorescent Bead Detection (no spectroscopy/40x Objective) Experiment: Direct excitation of PMT

15. Acknowledgments Dr Katsuo Kurabayashi Steven Truxal Dr. Sandrine Martin and Trasa Burkhardt National Science Foundation National Nanotechnology Infrastructure Network UMich 2008 REU Students Thank You For a Great Summer!

16. Why are Chicken McNuggets so Delicious? http://www.mcdonalds.com/app_controller.nutrition.categories.ingredients.index.html#2 http://en.wikipedia.org/wiki/Polydimethylsiloxane According to Wikipedia…

17. Thanks for listening! Questions?