Integrating Detection of Multiple Pathogens in Food

Saúde e medicina Tecnologia

Lawrence Goodridge Department of Animal Sciences Colorado State University [email_address] Integrating Detection of Multiple Pathogens in Food

Introduction ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Biochemical Assays Previously Developed Salmonella Listeria monocytogenes Escherichia coli

Disadvantages of the Biochemical Assays ,[object Object],[object Object],[object Object]

Paper-Based Testing Devices ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Lu et al. 2009

Assay Fabrication with Wax ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

L. monocytogenes Assay: Characterization ,[object Object],[object Object],[object Object],[object Object]

L. monocytogenes Assay ,[object Object],[object Object],[object Object],[object Object],Control 2 hr 3 hr 3.5 hr 6 hr 5 hr 4.5 hr 4 hr

Salmonella assay: pH Studies ,[object Object],[object Object],[object Object],[object Object]

Salmonella Assay ,[object Object],[object Object],[object Object],[object Object],[object Object],6.0 6.5 7.0 7.5 8.0 9.0 control

E. coli Assay: pH Studies ,[object Object],[object Object],[object Object]

E. coli Assay: Limit of detection ,[object Object],[object Object],Varying β -galactosidase (µg/mL) control 0.03 0.06 0.13 0.4 0.33 0.26 0.2

E. coli Assay ,[object Object],[object Object],Using 10 mL of growth media Using 1 mL of growth media for enrichment of E. coli (t = 4.5 hr) 7 mm

Putting it all together: Detection of all the Bacteria ,[object Object],[object Object],[object Object],[object Object],E. coli Salmonella spp. control L. monocytogenes

Future Work ,[object Object],[object Object],[object Object],[object Object],[object Object]

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