Ceribrospinal Fluid Analysis is a very important investigations in all laboratories. You can asses this ppt for your routine laboratory work.

1. Cerebrospinal Fluid Analysis BY BIJO AUGUSTINE

18. Clot/Coagulation formation Allow the specimen of CSF to stand over over night and examine the sample for fibrin clot, which is formed if the sample contains fibrinogen. Also note the nature of the clot. - Delicate clot, which resembles a cobweb, is characteristically seen in tubercular meningitis due to marked increased in CSF proteins. The clot may have entrapped tubercle bacilli, which could be demonstrated microscopically by staining for acid-fast bacilli.

19. Clot/Coagulation formation Corase clot is formed in pyogenic meningitis, traumatic tap and in case of complete spinal block. pH Determination. pH can be measured by using pH paper or using pH meter.

28. Reference Intervals for CSF

32. Immature cells in CSF

41. Test Appearance Pressure WBC/ μ L Protein mg/dL Glucose mg/dL Chloride Normal CSF Clear 90 – 180 mm 0-8 lymph. 15-45 50-80 115-130 mEq/L Acute bacterial meningitis Turbid Increased 1000 -10000 100 – 500 < 40 Decreased Viral meningitis Clear Normal to moderate increase 5-300, rarely >1000 Normal to mild increased Normal Normal Tubercular meningitis Slightly opaque cobweb formation Increased/ decreased, spinal block 100-600 mixed or lymph. 50-300 due to spinal block Decreased Decreased Fungal meningitis Clear Increased 40-400 mixed 50-300 Decreased Decreased Acute syphilitic Clear Increased About 500 lymph Increased but <100 Normal normal

42. Bacterial Meningitis

44. Neurosyphilis

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