![SPECIFICATION SHEET
Rev 092708A
Recombinant Staphylococcal Putative IgG-binding Protein
Staphylococcal, Recombinant (Protein A)
Expressed in E.Coli
Cat. No. Protein A-1390S
Lot. No. (See product label)
PRODUCT INFORMATION
Product Overview: Recombinant Staphylococcal Protein A Amino Acid Sequence: MAQHDEAQQN AFYQVLNMPN
produced in E.Coli is a non-glycosylated, Polypeptide chain LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAP-
containing 434 amino acids (37-469 a.a.) and having a mo- KADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNG-
lecular mass of 48.1 kDa. Recombinant Staphylococcal Pro- FIQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKE
tein A is purified by proprietary chromatographic techniques. QQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSE
Description: Protein A is a cell wall protein deriving from AKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE
Staphylococcus aureus which exhibits unique binding prop- EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNK
erties for IgG from a variety of mammalian species and for FN KEQQNAFYEI LHLPNLTEEQ RNGFIQSL KDDP SVS
some IgM and IgA as well. It binds with the Fc region of im- KEILA EAKKLNDAQA PKEEDNNKPG KEDNNKPGKE DN
munoglobulins through interaction with the heavy chain. It NKPGKEDG NKPGKEDNKK PGKEDNKKPG KEDNK
couples to a wide variety of reporter molecules including KPGKE DGNKPGKEDN KKPGKEDGNG VHVVKPGDTV
fluorescent dyes, enzyme markers, biotin, colloidal gold and NDIAKANGTT ADKIAADNKL ADKNMIKPGQ ELVVDKKQ
radioactive iodine without affecting the antibody binding site. PA NHADANKAQA LPET.
Recombinant Protein A was developed to increase the speci- Purity: Greater than 90.0% as determined by SDS-PAGE.
ficity of the molecule for IgG and is widely used both in re- Stability: Store at 4°C if entire vial will be used within 2-4
search and bioprocessing. The recombinant protein A is pro- weeks. Store, frozen at -20°C for longer periods of time. For
duced by expressing a modified protein A gene in E.coli. A long term storage it is recommended to add a carrier protein
specific purification process with strict quality control was (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
taken to get the recombinant protein A with the purity of more
than 98% , no human IgG affinity step is used during vali-
dated fermentation and purification and devoid of bacterial
contaminant found normally in native Protein A. (Free of GENE INFORMATION
Staphylococcus endotoxins and hemolysin).
Source: Escherichia Coli. Gene Name: Sca_1511 putative IgG-binding protein
Physical Appearance: Sterile filtered colorless solution. [ Staphylococcus carnosus subsp. carnosus TM300 ]
Formulation: The Protein-A protein solution contains 20mM Synonyms: Protein A; putative IgG-binding protein
Tris-HCl, pH-8 and 10% glycerol. GeneID: 7550673
FOR RESEARCH USE ONLY
Creative BioMart. All rights reserved.
45-16 Ramsey Road Shirley, NY 11967, USA
Tel: 631-559-9269 ·Fax:631-207-8356
E-mail: info@creative-biomart.com
www.creative-biomart.com](https://image.slidesharecdn.com/proteina-1390sproteina-111022072911-phpapp01/85/Protein-a-1390-s-protein-a-1-320.jpg)
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- 1. SPECIFICATION SHEET Rev 092708A Recombinant Staphylococcal Putative IgG-binding Protein Staphylococcal, Recombinant (Protein A) Expressed in E.Coli Cat. No. Protein A-1390S Lot. No. (See product label) PRODUCT INFORMATION Product Overview: Recombinant Staphylococcal Protein A Amino Acid Sequence: MAQHDEAQQN AFYQVLNMPN produced in E.Coli is a non-glycosylated, Polypeptide chain LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAP- containing 434 amino acids (37-469 a.a.) and having a mo- KADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNG- lecular mass of 48.1 kDa. Recombinant Staphylococcal Pro- FIQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKE tein A is purified by proprietary chromatographic techniques. QQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSE Description: Protein A is a cell wall protein deriving from AKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE Staphylococcus aureus which exhibits unique binding prop- EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNK erties for IgG from a variety of mammalian species and for FN KEQQNAFYEI LHLPNLTEEQ RNGFIQSL KDDP SVS some IgM and IgA as well. It binds with the Fc region of im- KEILA EAKKLNDAQA PKEEDNNKPG KEDNNKPGKE DN munoglobulins through interaction with the heavy chain. It NKPGKEDG NKPGKEDNKK PGKEDNKKPG KEDNK couples to a wide variety of reporter molecules including KPGKE DGNKPGKEDN KKPGKEDGNG VHVVKPGDTV fluorescent dyes, enzyme markers, biotin, colloidal gold and NDIAKANGTT ADKIAADNKL ADKNMIKPGQ ELVVDKKQ radioactive iodine without affecting the antibody binding site. PA NHADANKAQA LPET. Recombinant Protein A was developed to increase the speci- Purity: Greater than 90.0% as determined by SDS-PAGE. ficity of the molecule for IgG and is widely used both in re- Stability: Store at 4°C if entire vial will be used within 2-4 search and bioprocessing. The recombinant protein A is pro- weeks. Store, frozen at -20°C for longer periods of time. For duced by expressing a modified protein A gene in E.coli. A long term storage it is recommended to add a carrier protein specific purification process with strict quality control was (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during vali- dated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of GENE INFORMATION Staphylococcus endotoxins and hemolysin). Source: Escherichia Coli. Gene Name: Sca_1511 putative IgG-binding protein Physical Appearance: Sterile filtered colorless solution. [ Staphylococcus carnosus subsp. carnosus TM300 ] Formulation: The Protein-A protein solution contains 20mM Synonyms: Protein A; putative IgG-binding protein Tris-HCl, pH-8 and 10% glycerol. GeneID: 7550673 FOR RESEARCH USE ONLY Creative BioMart. All rights reserved. 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-559-9269 ·Fax:631-207-8356 E-mail: info@creative-biomart.com www.creative-biomart.com