protozoa-amoeba

7. CYST
IODINE STAIN

9. Trophozoites, unstained Not characterictic
Trophozoites, stained Diagnostic: nucleus with large
central karyosome surrounded
by a ring of small chromatin
granules; or nucleus structure
as in cyst
Cysts, unstained Suggestive: large refractile body
in cytoplasm; single nucleus
Cysts, stained Diagnostic: basket nuclei or
nuclei as in trophozoite; large
glycogen vacuole

10. Iron Hematoxylin
stain
I.bütschlii trophozoite showing large blotchy
karyosome withing nucleus. Nucleus outline visible
showing no peripheral chromatin.

11. Iron Hematoxylin
stain
I.bütschlii trophozoite - cell shape is more irregular
that Endolimax nana which is usually rounder.

12. Iron Hematoxylin
stain
I.bütschlii trophozoite - Binucleated cell

13. CYST
Nuclear membrane all but
invisible. Large glycogen vacuole seen
on right side of cell.

14. CYST
Large glycogen vacuole takes up most of
the cell's interior

15. CYST
binucleated cell

16. CYST
Concentrate (unstained)

17. CYST
BASKET OF FLOWERS, ESTER
BASKET

18.  The most common of the smaller
intestinal amebae
 usually encountered with about the same
frequency as in E. coli
 Size range for both trophozoites and cyst
is similar to that of E. hartmanni [(usual
size, 4-12 µm) and cyst forms (usual
size, 5-10 µm)], thus makes confusion in
unstained preparations
 Epidemiology is cosmopolitan
 Colonizes the colon

22. Trophozoites, unstained Not charateristic
Trophozoites, stained Diagnostic: a nucleus with large
karyosome, generally with little or
no peripheral chromatin
Suggestive: Ovoid
Cysts, unstained Suggestive: Ovoid
Cysts, stained Diagnostic: 4 nuclei with large
karyosome and little or no
peripheral chromatin

23. Trichrome stain
The nuclei in this mature, quadrinucleated cyst
have a large karyosome surrounded by a clear
halo; peripheral chromatin is absent.

24. Wet mount
examination of SAF-
fixed specimen
The nuclear characteristics of this trophozoite
are clearly visible

25. VIDEO PRESENTATION
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