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WHAT IS DNA
       FINGERPRINTING?
0Did you know that your DNA has a
 “fingerprint”?
0It is can be used to identify a person
0If you like shows like CSI then you may
 have heard of identifying people by their
 DNA
0That is done through DNA fingerprinting
0All you need is some DNA
How do you get a DNA
        fingerprint?
0First you need some DNA
0Through a process of cutting the DNA
 with a restriction enzyme (remember
 enzymes from Unit 5?) and then putting
 the DNA through Gel
 Electrophoresis, the DNA makes a
 “fingerprint”
0Gel Electrophoresis – uses gel and
 electricity to separate the DNA
What are some uses of DNA
     fingerprinting?

0Solving crimes
0Determining paternity
This is what a DNA
fingerprint looks like….
Parents share…
 0Bands in the DNA fingerprint with their
  children
 0Each band in the child’s DNA will be the
  same as either their mother and their
  father

In forensics, criminals will have matching
 DNA fingerprinting to the DNA found at
          the scene of the crime
Who is the
           criminal???

        The DNA from
          the scene
        matches Dino




Online Images. Access Excellence. 13 May 2007.
http://www.accessexcellence.org/AE/AEPC/WWC/1994/fly_forensics.html
Look at this old
EOC
question!!!
The answer is
suspect C.
They have the
same bands!
Results from a single locus
probe DNA fingerprint
analysis for a man and
woman and their four
children are shown in the
autoradiograph to the right.
Which child is least likely to
be the biological offspring of
this couple?

A.   Child 1
B.   Child 2
C.   Child 3
D.   Child 4
This was a tricky
problem. If child 2 is
the child of this man
and woman, child 2
must have one band
from each of them.
However, child 2
appears to share two
bands with the
mother and none
from the "father."
Obviously, the
mother could not
have contributed
both bands (alleles)
to her child.

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Dna fingerprinting

  • 1.
  • 2. WHAT IS DNA FINGERPRINTING? 0Did you know that your DNA has a “fingerprint”? 0It is can be used to identify a person 0If you like shows like CSI then you may have heard of identifying people by their DNA 0That is done through DNA fingerprinting 0All you need is some DNA
  • 3. How do you get a DNA fingerprint? 0First you need some DNA 0Through a process of cutting the DNA with a restriction enzyme (remember enzymes from Unit 5?) and then putting the DNA through Gel Electrophoresis, the DNA makes a “fingerprint” 0Gel Electrophoresis – uses gel and electricity to separate the DNA
  • 4.
  • 5. What are some uses of DNA fingerprinting? 0Solving crimes 0Determining paternity
  • 6. This is what a DNA fingerprint looks like….
  • 7. Parents share… 0Bands in the DNA fingerprint with their children 0Each band in the child’s DNA will be the same as either their mother and their father In forensics, criminals will have matching DNA fingerprinting to the DNA found at the scene of the crime
  • 8. Who is the criminal??? The DNA from the scene matches Dino Online Images. Access Excellence. 13 May 2007. http://www.accessexcellence.org/AE/AEPC/WWC/1994/fly_forensics.html
  • 9. Look at this old EOC question!!!
  • 10. The answer is suspect C. They have the same bands!
  • 11. Results from a single locus probe DNA fingerprint analysis for a man and woman and their four children are shown in the autoradiograph to the right. Which child is least likely to be the biological offspring of this couple? A. Child 1 B. Child 2 C. Child 3 D. Child 4
  • 12. This was a tricky problem. If child 2 is the child of this man and woman, child 2 must have one band from each of them. However, child 2 appears to share two bands with the mother and none from the "father." Obviously, the mother could not have contributed both bands (alleles) to her child.