10. Vps34 Vps15 Rab5 GTP PI[3]P Christoforidis et al., Nature Cell Biol. 1999 Murray et al., Traffic (2002) hVps34 Regulates Early Endosomal Fusion P P hVps34 Class III PI[3]P PX FYVE hVps15
11. Former Labmates Maya Byfield James Murray Gary Gerfen Mark Girvin Ana Maria Cuervo Invasion and Motility PO1 Anne Bresnick Diane Cox John Condeelis Jeff Pollard Jeff Segall Richard Stanley Roger Williams (Cambridge) Somasekar Seshagiri (Genentech) Tom Neufeld (Univ. Minnesota) Kevan Shokat (USCF) Bart Vanhaesebroeck (London Sch. Med.) Funding: NIH, NCI, ADA
12. CCV EE RC LE Lysosome TGN Plasma Membrane 2 1 3 4 5 5 6 Is mTORC1 Inhibited by Disruption of the Endocytic System? Mutant Effect 1) Rab5 CA Inhibit 2) Eps15 No Effect 3) Rab11 Mutants No Effect 4) Rab7 Mutants No Effect 5) PIKFyve DN No Effect 6) SKD1 DN No Effect
13. Constitutive activation of Rab5 blocks mTORC1/S6K1 activation AA Stimulated S6K1 in HEK293T Flinn et al. Mol. Biol. Cell. (2010)
14. CCV EE RC LE Lysosome TGN Plasma Membrane 2 1 3 4 5 5 6 Is mTORC1 Inhibited by Disruption of the Endocytic System? Mutant Effect 1) Rab5 CA Inhibit 2) Eps15 No Effect 3) Rab11 Mutants No Effect 4) Rab7 Mutants No Effect 5) PIKFyve DN No Effect 6) SKD1 DN No Effect