Pig Lameness and Diagnostic Lab Views - Dr. Darin Madson, Veterinary Diagnostic & Production Animal Medicine, College of Veterinary Medicine, Iowa State University, from the 2013 Allen D. Leman Swine Conference, September 14-17, 2013, St. Paul, Minnesota, USA.
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Dr. Darin Madson - Pig Lameness and Diagnostic Lab Views
1. Feeding for Optimal Bone Strength
2013 Allen D. Leman Swine Conference
What we are seeing at the
diagnostic laboratory
Darin Madson
madson@iastate.edu
Veterinary Diagnostic Laboratory
Iowa State University
2. What we are seeing
โข Increased clinical investigations/submissions
โข Suggesting an issue or production concern
โข Different from โnormal/routineโ submissions
โข Suboptimal clinical lameness assessments
โข With improper sample submission
โข Wide variation in bone quality
โข The gamut of lameness etiologies found
โข Metabolic condition, OCD, and Mycoplasma sp.
Veterinary Diagnostic Laboratory
Iowa State University
3. Bone โ โthe organโ
โข Bone is constantly being remodeled
โ Throughout life; even after growth as stopped
โ Bone is dynamic ๏ tells a story!
โข
โข
โข
โข
Mechanical stress
Decreased nutrition
Environmental stress
Disease
โ Can be seen microscopically
โข Arrest and reversal lines
Veterinary Diagnostic Laboratory
Iowa State University
4. What is metabolic bone disease?
โข Disturbances related to:
1.
2.
3.
4.
Vitamin D
Can be combinations of these
Calcium
Phosphorus
Parathyroid hormone
โข Results in
1. Depletion of bone mineral storage
2. Delayed formation/modeling
Veterinary Diagnostic Laboratory
Iowa State University
5. What is metabolic bone disease?
โข Terminology
โ Rickets
โข Abnormal endochondral ossification in a growing animal
โ Defective mineralization of growing bone
โ Osteomalacia
โข Similar to rickets, but in adult animals
โ Defective mineralization of bone remodeling
โ Fibrous osteodystrophy
โข Extensive bone resorption with replacement by fibrous connective
tissue
โ Osteoporosis/Osteopenia
โข Reduced bone mass
โ Quality of remaining bone is normal
Veterinary Diagnostic Laboratory
Iowa State University
6. What is metabolic bone disease?
Common mechanisms:
1. Inadequate dietary supplementation of vitamin D3
2. Inadequate absorption of phosphorus due to low
phosphorus in diet, phosphorus bound to phytate and
therefore unavailable, and inadequate or ineffective
phytase usage
3. Imbalance of feed calcium to phosphorus ratio; improper
formulation of Ca:P ratio in diet (should be roughly 1.2:1)
4. Inadequate dietary calcium can also contribute to rachitic
lesions, though these are also often confounded by
accompanying osteoporosis.
Veterinary Diagnostic Laboratory
Iowa State University
7. Potential industry issues
โข Swine diets ๏ maximum growth
โข Not bone strength
โข Ingredient costs
โข Alternative feedstuffs
โข Variability in feedstuffs
โข Amount of ingredient
โข Process for inclusion (pelleting)
โข NRC values (outdated?)
Veterinary Diagnostic Laboratory
Iowa State University
8. Clinical signs
Metabolic bone disease
โข Clinical signs are variable
โข Sudden death (found dead)
โข Tremors/seizures (CNS like disease)
โข Muscle fasciculations/tetany
โข Weakness
โข Lameness
โข Painful gait and reluctancy to move
โข Bone fractures
Veterinary Diagnostic Laboratory
Iowa State University
11. Diagnostic testing
โข Assessing rib strength
Rickets (growing pigs)
Phosphorus
Rubbery bones
Calcium
Fragile bone; Break easy, but snap
Vitamin D
Rubbery bones
Veterinary Diagnostic Laboratory
Iowa State University
12. Diagnostic testing
โข Bone analysis
โ Need to know the processes
โข Wet weight or dry-fat free!!!
Dry, fat free
Wet weight
Swine; rib bone
Bone ash
58-62 %
Bone density
1.4-1.5 g/ml.
Bone ash Calcium
32-39%
Swine; rib bone
Bone ash
25-30 %
Bone density
1.4-1.5 g/ml.
Bone ash Calcium
32-39%
Bone ash Phosphorus 13-22%
Bone ash Phosphorus 13-22%
Veterinary Diagnostic Laboratory
Iowa State University
13. Diagnostic testing
Wet weight
Bone Ash - Summary of 169 porcine cases
Above noraml (>35%)
Normal (25-35%)
Total
Deficient (below 25%)
0
20
40
60
80
100
number of test results within each range
Veterinary Diagnostic Laboratory
Iowa State University
14. Diagnostic testing
โข Serum
โ Calcium
โ Phosphorus
โ Alkaline phosphate (ALP)
ยป Increased with bone resorption
Analyte
Vit D
deficiency
Calcium
deficiency
Phosphorus
deficiency
Calcium
โฌ
โฌ, โฌ
โฌ
โฌ
โฌ, โฌ
โฌ
โฌ, โฌ
โฌ
โฌ
Phosphorus
ALP
Veterinary Diagnostic Laboratory
Iowa State University
15. Diagnostic testing
โข Serum Vitamin D
โ Heartland Assays. Inc (Ames, Iowa)
โ Ron Horst
Darinโs Thought
Age of animal
Neonate
10 days
3-4 weeks old
Finishing pigs
Mature
Parturition
25-OH-D3 ng/ml
5-15
8-23
25-30
30-35
35-70
35-100
Stay above 15 ng/ml
Veterinary Diagnostic Laboratory
Iowa State University
17. Vitamin D research - ISU
โข Main goal
โ Assess vitamin D levels across different ages of pigs in the
Midwest
โข Two different time points
โ Once in January (2011)
โ Once in July (2011)
โข Ages of animals assessed in the surveillance
โ
โ
โ
โ
โ
3 week old pigs
~11 week old pigs
Market hogs
Gestation sows
Boars
8 samples from 15 sites in each
age group at each time point
1,200 total samples tested
600 samples tested in January 2011
600 samples tested in July 2011
Veterinary Diagnostic Laboratory
Iowa State University
18. Results
**
*
35 ng/ml
15 ng/ml
5 ng/ml
Veterinary Diagnostic Laboratory
ANOVA - * & ** indicate significant differences between January and June samples (p < 0.01)
Iowa State University
20. Vitamin D research โ ISU take 2
Objective of proposal
โ Assessing the quality of vitamin D from
multiple suppliers overtime
โข 5 feed manufactures
โข Sample bulk vitamin D 1x per month
โ Mixed from multiple locations
โข Sampling for 12 months
โ October 2011-September 2012
Veterinary Diagnostic Laboratory
Iowa State University
21. Vitamin D research โ ISU take 2
โข Results
โ No company was found
to be significantly lower
than 500,000 IU
โข No difference in US vs.
Foreign vitamin D
manufactures
โ Month was found to
be significant
โข P= 0.0022
โข Storage???
Veterinary Diagnostic Laboratory
Iowa State University
22. Confounding issues
โข Lameness failures
โ Clinical assessment
โ Age, weight, feed, growth rate, and watching them walk
โ Gross assessment
โ It is more than hacking off a limb
โ Sample submission is key
โ Complexity
โ Correct samples
โ Other differentials
โ Knowing what to look for
Veterinary Diagnostic Laboratory
Iowa State University
25. Take home points
โข Lameness is an industry issue
โ Multiple factors are associated
โข Infectious or nutritional
โข The โinsultโ may have occurred previously
โข Clinical assessments are important
โข Multiple samples are needed
โข Location can vary significantly in clinical pigs
โข Understand potential confounding problems
โ Are the findings the โrealโ cause of the lameness
Veterinary Diagnostic Laboratory
Iowa State University
26. Thank you for your attention!
QUESTIONS?
Veterinary Diagnostic Laboratory
Iowa State University