1.
When performing the TLC analysis for separation of amino acids it is important to avoid
touching the slide because Select one: a. you may damage the mobile phase b. chemicals on your
hand may be transferred to the slide c. chemicals on your hand may react with the test solutions
d. the coating on the slide is hazardous
Solution
The correct option is B that is chemicals on your hands may be transfered to the slide.
Our hands contains various oils present in skin and by touching the plates of tlc the oils may get
transffered and the plate may get contaminated as a result of which varoius other spots also
become visible apart from the amino acids while the solvent system runs .