WHAT IS EBOLA?
Ebola is the most lethal virus known to man.
Ebola hemorrhagic fever is a very contagious illness that is often fatal in humans and nonhuman primates (monkeys, gorillas, and chimpanzees).
3. CONTENTS
INTRODUCTION
EPIDEMIOLOGY
CLASSIFICATION OF THE VIRUS
MORPHOLOGY UNDER ELECTRON MICROSCOPE
PATHOPHYSIOLOGY
SYMPTOMS
MODE OF TRANSMISSION
RISK FACTOR
DIAGNOSIS AND IDENTIFICATION
PREVENTION AND CONTROL
PROGNOSIS
TRATMENT
4. INTRODUCTION
WHAT IS EBOLA?
Ebola is the most lethal virus known to man.
Ebola hemorrhagic fever is a very contagious illness that is often fatal in
humans and nonhuman primates (monkeys, gorillas, and
chimpanzees).
It kills 50 to 90 percent of the infected personnel, compared to Anthrax’s
25 percent.
Outbreaks occurred all over central Africa.
Believed to be primate deadly. Monkeys have infected many doctors,
scientists, and monkey care handlers.
5. EPIDEMIOLOGY
Ebola Hemorrhagic Fever was first found in 1976
It struck two countries within that year
Sudan – in a town called N’zara
Zaire, now known as the Democratic Republic of Congo
In these two instances the mortality rate was between
50 –90%.
Following those epidemics, Ebola hit Africa in many other instances
the worst yet being in the year 2000 when it struck Uganda infecting
more than 400 people.
6. CLASSIFICATION OF THE VIRUS
Order: Mononegavirales
Family: Filoviridae
Genus: Ebolavirus
Ebola virus is one of two members of a family of RNA viruses called the
Filoviridae. There are four identified subtypes of Ebola virus. Three of
the four have caused disease in humans:
Four types of strains they are:
• Ebola-zaire
• Ebola-sudan
• Ebola-cote ‘d-Ivoire
• Ebola-reston
7. MORPHOLOGY UNDER ELECTRON
MICROSCOPE
Single stranded , linear, non-segmented
Negative-sense RNA (encoded in a 3’-5’ direction)
Coiled RNA in spike-covered envelope from host cell
Therefore ,spreads rapidly.
appears to have “spikes” due to glycoprotein on
outside membrane
8. PATHOPHYSIOLOGY
Endothelial cells, mononuclear phagocytes, and hepatocytes are the
main targets of infection.
The GP forms a trimeric complex, which binds the virus to the
endothelial cells lining the interior surface of blood vessels. The sGP
forms a dimeric proteins which interferes with the signaling of white
blood cell.
These white blood cells also serve as carriers to transport the virus
throughout the entire body to places such as the lymph nodes, liver,
lungs, and spleen.
9.
10. SYMPTOMS
Incubation period: 2-21 day
Sudden onset of fever
Intense weakness
Muscle pain
Headache and sore throat
Impaired kidney and liver function
11. MODE OF TRANSMISSION
Transmission:
• Unsterilized needles
• Suboptimal hospital condition
• Personal contact
• Transmitted through body fluids, secretions
• Ebola-Reston has shown to be air borne.
12. RISK FACTORS
Antibodies against Ebola
Ebola Gene sequences in liver and spleen
Fruit bats do not show any symptoms
Best candidate to be the reservoir
More research needs to be done.
13. DIAGNOSIS AND IDENTIFICATION
Clinical diagnosis:-
• Difficult because early symptoms
(red eye,skin rash) are nonspecific to virus
• Takes a combination of many symptoms characteristic of Ebola.
Laboratory testing/Diagnosis:-
• Antigen-capture enzyme-linked immunosorbent assay(ELISA)
IgM ELISA test
• polymerase chain reaction (PCR)-a DNA test to match the DNA from
the sample to known Ebola DNA
• All done with in a few days of onset of symptoms.
14. DIAGNOSIS AND IDENTIFICATION
Advance testing :
• Test for IgM and IgG antibodies.
Immunohisto chemisty testing
• Virus Isolation
• PCR
Immunofluorescence assays are used to confirm Ebolavirus presence
in cell cultures.
15. PREVENTION AND CONTROL
Hospitals must follow precautionary method such as:
• Wearing gloves
• Isolating infected individuals
• Practicing nurse barrier technique
• Proper sterilization and disposal of all equipment
• Burials must be done correctly
• No washing or touching carcass
• Put into body bags and bury outside city.
16. PREVENTION AND CONTROL
• Due to the extreme biohazard, doctors must wear Level 4 containment
suits. They are the equivalence of a spacesuit.
• Some poor African towns put the diseased in a straw hut, and then
burn it down when they’re dead. Simple yet effective.
17. PROGNOSIS
As the Ebola virus is of the most deadly illnesses known to humankind,
ebola virus patients have little chance of surviving. The definitive
prognosis of the Ebola virus is death, and as many as 90% of people
afflicted with the disease die from the shock that it causes to the body.
Morbidity and mortality rates are very high, and they vary with the
strain of Ebola. The most highly lethal Ebola subtype is EBO-Z, which
has been reported to have a mortality rate as high as 88%.TheEBO-
S subtype has a reported mortality rate of 50%.
18. TREATMENT
THERE IS NO CURE FOR EBOLA HF
• Care of Infected Person.
• Oxygen and devices that help with breathing
• Antibiotics to prevent secondary infections from bacteria
• Medications to control fever, help the blood clot, and maintain blood pressure
Intravenous (IV) fluids to maintain fluids and electrolytes (sodium,potassium,
and chloride)
• Good nursing care, Bleeding problems may require transfusions of platelets or fresh
blood.
EXPERIMENTAL TREATMENT:
In the Kikwit outbreak in DRC, doctors transmitted blood from survivors
to sufferers, hoping to transmit whatever antibodies helped them survive.
19. FUTURE OUT LOOKS
A study released in December of 2003 showed that researchers studying
infected monkeys have found a way to increase survival rates
100% of infected monkeys had been dying
These were injected with rNAPc2, a factor known to inhibit blood
coagulation, a characteristic of Ebola HF
33% of these monkeys survived and regained health. All untreated monkeys
died.
rNAPc2 is known to be relatively safe in humans – this method is being
studied further
20. REFERENCES
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