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INTRODUCTION
Mushroom cultivation is the most suitable technology for creating
wealth out of plant, animal and industrial waste. Which are abundantly
available all over the globe. Mushroom cultivation is the gaining momentum
worldwide because of dietary food protein.
Mushroom cultivation is unique in the sense that it is the most
efficient

and

economically

valuable

technology

for

conservation

of

lignocelluloses material into high quality protein food. Mushroom cultivation
can subsidies income of millions of farmers.
Oyster mushroom is known as the wood fungus and in India is
commonly known as “Dhingri”. Oyster mushroom is scientifically known as
Pleurotus spp. Pleurotus is Basidiomycetes fungus belonging to order
Agaricles and family Trichomataceae/Polyporaceae. There are about 38
species described under genus Pleurotus from different part of the world, out
of which 25 species are under cultivation. They mainly include Pleurotus
osteatus, Pleurotus eryngii, Pleurotus flabellatus, Pleurotus sajar caju,
Pleurotus sapidus, Pleurotus membranaceous, Pleurotus citrinopileatus,
Pleurotus eous and Pleurotus florida, P. cornicorpae.
Pleurotus sajar caju was first reported by Jandaik and Kapoor in
1974, can be grown in the temperature range of 20-300C with 70-85per cent
humidity, it has a good biological efficiency 84-90per cent under optimum
condition. Oyster mushroom contains 90per cent moisture , 4-5per cent
carbohydrate on wet weight basis, 20-35per cent protein, low sugar, low fat,
low starch, high fibre and sodium potassium ratio. They contain good
amount of C and B complex (thiamine, riboflavin and niacin). It also

1
contains minerals, iron (Fe), Phosphorus (P), Potassium(K) and sodium(Na)
and also low but available form of Iron(Fe).
The Oyster mushroom Pleurotus spp. is saprophytic fungus
commercially cultivated throughout the

world

because

of

its

tasty

basidiocarp and simple cultivation technology. Oyster mushroom is having
capacity to grow in tropical and subtropical region. It is being cultivated in
Himachal Pradesh, Uttar Pradesh, Andhra Pradesh, Jammu and Kashmir,
Karnataka, Goa, Tamil Nadu and Panjab (Chandra and Sharma in 1995).
Oyster mushroom can be use in different kitchen items such as
pickle, chutney, pakoda, bhaji, pulav, mix vegetables, kofta and other
delicious items etc.
Its cultivation is popular due to its rapid mycelial growth,
pleasant flavour, wide adoptablity and simple method of cultivation.

Objectives:1)

To learn various production techniques of mushroom
production.

2)

To get practical and experimental oriented knowledge of
mushroom production.

3)

To increases the yield of Pleurotus sajar caju by
supplementation of different supplements.

4)

To prepared value added products from fresh mushroom.

2
REVIEW
Mandeel et.al (2005). Cultivated three spp. of Pleurotus viz., P.
columbinus, P.sajar-caju and P. ostreatus on organic waste such as chopped
paper,cardboard, sawdust and plant fibres. Higher yield of P. columbinus
was obtained on cardboard (135.5 g) followed by paper (100.8 g). P.
ostreatus produced maximum yield on paper (112.4 g) however, P. sajar-caju
failed to lowest yield on unsupplemented paddy straw (B.E 70 per cent).
Islam et.al.(2009).Evaluated performance of Pleurotus flabellatus on
seven different types of substrates viz., Mango, Jackfruit, Coconut, Jam,
Kadom, Manogony, Shiris sawdust with wheat bran and CaCO3. The
maximum biological efficiency was obtained with mango saw dust (150gm),
followed

by

Monogony

(148gm),

Shiris

(146gm),

Kadom

(136gm),

Jam(114gm), Jackfruit (97gm) and Coconut saw dust (83gm). Coconut saw
dust

proved

to

be

the

poorest

substrate

for

fruiting

of

this

mushroom.produced satisfactory yield on all the substrates under study.
Indira et.al (2010). Studied the effect of Agnihotra on yield of P.
sajor-caju.

In this experiment paddy straw was supplemented with

Agnihotra ash ,Groundnut seed cake, Horse gram

powder, Cotton seed

powder among this supplement Agnihotra ash recorded higher (B.E. 97.5
per cent) yield in paddy straw while.
Chiejinas et.al (2010). Studied the effect of seven substrates for
cultivation of Pleurotus tuberregium. The substrates used were top soil, river
sand, Fermented sawdust, Fermented oil palm fruit fiber, Mixture of top soil
and Fermented sawdust, mixture of fermented oil palm fruit fibre and
fermented sawdust, mixture of river sand and fermented sawdust. Among
the seven substrates river sand and sawdust mixture produced significantly
more yield.
3
Abere and Stanley (2011). Cultivated P.Tuber-regium on corncob
supplemented with rice bran at 10, 20 and 30 percent concentration on dry
weight basis. 30per cent supplementation of rice bran gave higher yield as
compared to other treatments.

4
LOCATION
i.
ii.

Name of Department:- Department of Plant Pathology
Name of College :- Sharadchandraji Pawar College
of Agriculture, Kharwate-Dahiwali.
Tal.- Chiplun, Dist.- Ratnagiri.

iii.

Name of University :- Dr. Balasaheb Sawant Konkan
Krishi Vidhyapeeth, Dapoli.

WORKING PERIOD
1 December 2011 to 4 April 2012

5
INPUT DETAILS: (Quantity and Cost of input)
A. For preparation of spawn (For 80 beds):
Sr.No Inputs
Quantity
1
2
3
4
5
6

Wheat grain
CaCO3 powder
Gas
Electricity
Cotton
bags

Cost (Rs.)

5kg
400gm
2 units
90 bags

115
100
5
8
10
2

Total

240

Cost required for preparation 8 kg spawn is Rs.240.

B. Cost required for preparation of one bed:
Sr.No Inputs
Quantity
1
2
3
4
5

500gm
25gm
1
-

0.50
0.50
0.50
2
1

100gm

9

Total

6

Paddy straw
Supplements
Bags
Labour cost
Other(Detol,
rubber,Pin)
Spawn

Cost (Rs.)

13.50

For preparation one bed Rs. 13.50 Required

C. Cost required for preparation of 80 beds:
Sr.No Inputs

Quantity

1
2
3
4
5

40
80
1 bottle
1 packet

40
40
160
24
7

Total

271

Paddy straw
Bags
Labour cost
Detol
Rubber

Cost required for preparation of 80 beds is Rs.271

6

Cost (Rs.)
D. Supplement required for preparation of 50 beds:
Sr.No Supplement
Quantity (g) Cost (Rs.)
1

3
4

500

9

250

15

250

6

-

-

Total

2

Wheat
bran(10
per
cent)
Gram dal flour(5 per
cent)
Cotton seed cake(5 per
cent)
Groundnut seed cake(5
per cent)

30

Cost required for supplementation of 25 beds is Rs. 30
E. For marketing of mushroom bags required:
Total cost of bags- Rs. 8
F. Input
Sr.No
1
2
3
4

used for preparation of mushroom pakoda
Items
Quantity (g) Cost (Rs.)
Mushroom
125
30
Gram flour
100
7
Baking
According to 2
powder,salt,turmeric taste
powder, red chilli
oil
250
10
total
47

Cost required for preparation of mushroom pakoda is Rs.47

7
G. Input used for preparation of mushroom pulav
Sr.No Items
Quantity (g)
1
2
3
4
5
6
7
8
9
10

Mushroom
Rice
Onion
Cumin seed
Cardamom
Bay leaf
Clove
Black pepper
Ghee
salt

125
100
1
½tables spoon
2
2
2
2
2 tables spoon
According to taste
total

Cost
(Rs.)
30
3
1
9

9
1
53

Cost required for preparation of mushroom pulav is Rs.53
H. Total input cost required for mushroom cultivation
Sr.No Items
Cost
1
2
3
4
5

Spawn preparation
Preparation of 80 beds
Supplements of 25 beds
Dehydration of mushrooms
Packaging bags
total

Total input
Rs.486.75/-

cost

required

8

for

106
271
30
30
486.75

mushroom

cultivation

is
SUMMARY OF WORK PERFORMANCE
Mushroom is ideal agent for conversion of agro waste into
protein rich material. Among all the Pleurotus spp. is having ability to
grow in temperature range of 20-300C, its substrate colonization time
is late and it is more delicious than most of the species.
Oyster mushroom was grown on paddy straw. Paddy straw
are easily available in Konkan region, it act as good substrate for
mushroom cultivation. So that paddy straw was used for cultivation of
Pleurotus sajar caju. Mushroom beds were treated by using paddy
straw substrate supplemented with different supplements such as,
wheat bran (10per cent on dry weight basis substrate), cotton seed
cake, groundnut seed cake, gram dal flour (5 per cent on dry weight
basis of substrate). Among the substrate supplement combination tied
highest yield was observed in paddy straw supplemented with cotton
seed cake (610 g/500g dry substrate) that followed by paddy straw
supplemented with wheat bran

(600 g). However lowest yield was

observed in controlled treatment (Unsupplemented paddy straw).
From fresh mushroom we prepared value added products
such as mushrooms pakoras, mushroom pulav, mushroom pickle,
mushroom bhaji.

9
FLOWCHART
Steps involve in preparation of spawn
Good quality wheat grains

Clean and dry wheat grains

Soaked in water for (8-10 hrs)

Boiled the wheat grains up to they become soft(Avoid splitting)

Remove excess water

Spread on plastic sheet and add 4% CaCo3

Fill in conical flasks
Plugged with non-absorbent cotton

Kept in autoclave at 15 lbs pressure at 121◦C for 15 min.
Remove it from autoclave

Inoculation with pure culture

Incubate at room temperature (25±20C) for 1 week

Commercial spawn
10
Steps involve in preparation of Mushroom bed

Raw material (paddy straw)

Chopping (3-5 cm)

Fill up in gunny bags

Soaking in water (8-12 hrs)

Drain off excess water

Dip in hot water (80-85◦C) for 1-2 hrs

Drain of excess water

On cooling to room temperature bags with filled in layers of substrate,
supplement and spawn

Spawn running (15-20 days)

Remove plastic bags cover and maintain humidity, light and ventilation

Pinhead stage (3-5 days)
11
Cropping fruit flush, (3-4 days duration after pinhead) and subsequent
flushes about 10 days interval

Picking on maturity (before spore shedding start)

Marketing

Fresh/dried

12
MUSHROOM PAKODA

Fresh mushroom (125gm)

Cleaning and washing with water

Cut into transverse pieces

Mix them with gram flour, salt, turmeric powder, red chilli powder and
baking soda

Add little water

Heat oil

Fry mushroom pakodas till it become light brown

13
MUSHROOM PULAV
Good quality mushroom (125g)

Cleaning, washing and cutting in small pieces

Fry lightly in ghee and kept aside

Add cumin, cardamom, cloves, black pepper and bay leaves in ghee

Add onion and fry upto light brown colouration

Add rice and double quantity water and salt to taste

Add mushrooms after boiling of rice

Cook on slow fire for few minute

Serve in plates

14
MUSHROOM CUTLET

Fresh good quality of mushroom (125gm)

Clean and cut them into small pieces

Boiled the peeled green peas and potato make it into a paste

Cut the onion, green chillies, coriander leave into small pieces]

Grind the ginger into paste

Mixed all the mixture and add turmeric powder, chili powder and salt

Make the mixture into ball

Dip the ball in gram flour paste

Fry it in oil

Serve in plates

15
METHOD OF PRODUCTION
Maintenance of culture:
The pure culture of Pleurotus sajar caju was obtained from
the Department of Plant Pathology, College of Agriculture,Dapoli. The
culture was maintained on potato dextrose agar medium and sub
cultured at regular interval of three weeks.
Preparation of spawn
Spawn of pleurotus sajor

caju was prepared on wheat

grains. The grains were cleaned and boiled in hot water for 20-30
minutes taking care that the grains did not burst. After boiling, these
grains were spread on filter paper for removing the excess water.
Calcium carbonate@ 3-4per cent (wet weight basis) was mixed
uniformly with grains and then filled into 500 ml milk bottles (each
bottle contain 300gm boiled wheat grains). The bottles were plugged
with non absorbent cotton and sterilized in an autoclave at
1.54kg/cm2 pressure for one hour. The bottles were inoculated with
equal mycelial bits obtained from pure culture and incubated at room
temperature (27±1◦C) for 10-15 days.
Preparation of substrate
Paddy straw is common waste farm in Konkan region also it
obtained in low cost. Paddy straw contains high percentage of cellulose
so that it was use as a substrate for cultivation of Pleurotus sajor caju.
Paddy straw used for cultivation was stiff, clean and undamaged. The
substrates was first chopped into 2”-3” pieces then filled in gunny
bags and soaked overnight in cold water. 500 gm dry substrate was
used for preparing one mushroom bed. After overnight soaking weight
of 500 gm dry substrate increases to 2 kg wet substrate. Therefore one
bed comprised of 500 gm dry substrate i.e.2 kg wet substrate. The
gunny bag congaing soaked substrate was then transferred to plastic
16
drum with lid. Then hot boiling water was poured in the drum is such
a way that the substrate was thoroughly in hot water. The lid was then
placed on the drum to facilitate pasteurization.
Preparation of supplements:
Flour protein rich supplements viz., wheat bran, cotton seed
cake, groundnut seed cake and gram dal flour were used for
increasing the yield and increasing biological efficiency of Pleurotus
sajor caju. Among the flour supplements, wheat bran was used at
10per cent flour of dry weight of substrate while cotton seed cake,
gram dal flour and groundnut seed cake were used at 5per cent level.
Prior to use, required quantities of the supplements were sterilized by
autoclaving. Supplements were added to substrates at the time of
spawning.
Bed filling and maintenance
After preparation of substrates and supplements by the
above procedure, mushroom beds were prepared by using appropriate
quantity of each substrate with each supplement. Two kg wet
substrate can be properly accommodated in plastic bag (100 gauges) of
size 55X35 cm. Hence these bags were used for filling beds. The beds
were prepared by filling the layers of substrate, supplements and
spawn in a sequence. Rate of spawning was two percent on wet weight
basis of substrate. Filled plastic bag were then perforated on all side to
provide proper aeration. Darkness favours rapid colonization of the
substrate. Therefore these plastic bags were kept in the darkness at
room temperature. Substrate colonization period range between 15-20
days depending upon the temperature and substrates supplement
combination.

17
When the substrate was fully colonize, beds were opened
and hanged to a firm support. Beds were watered as and required by
giving gentle sprayer of water. Humidity in cropping rooms was
maintained between 82-85 per cent.
After first harvest, each bed was trimmed on the all side by
a sharp knife. By maintaining 80-85per cent humidity, second flush
was taken within a period of 10-12 and third flush 10 days after the
second.
Observation on biological efficiency were recorded by the
formula,
Biological efficiency =

Fresh weight of mushroom

X 100

Air dried substrates
(Chang et.al, 1981)
Different recopies of mushroom
Mushroom pakoras
Mushroom

-125g

Gram dal flour

-100g

Backing powder

- a pinch

Salt, turmeric powder, chilli powder-According to test
Oil to fry

-250g

First take the 300 g mushroom then clean, washed and cut
into transverse pieces. Mushroom pieces were mixed with gram dal
flour, salt, turmeric powder, red chilli powder and baking soda as per
required amount with little water for preparation of butter. After that
oil was heated and fried mushroom pakoras at a time till they become
brown.

18
Mushroom pulav
Mushroom

-125gm

Rice

-100g

Onion

-1

Cumin seed

-

Cardamom

-1

Bay leaf

-2

Clove

-1 to 2

Black pepper

-2 to3

Ghee

-2 table spoon

Salt

-according to taste

table spoon

The good quality of mushroom were selected for preparation
of pulav. Mushroom were cleaned and washed with water and cut into
small pieces. Then mushroom were fried in ghee. Afterfrying kept it
aside. In some ghee add cumin, coriander, cardamom, clove, black
pepper and bay leaves. When cumin seed where being to crackled
onion was added and tied till light brown color appeared then rice were
added with double quantity of water and salt for taste. After boiling of
rice, fried mushroom pieces were added in rice. Then cooked on slow
fire till the pulao was ready.

19
QUANTITY OF PRODUCE
Treatment
Paddy
straw
+
cotton
seed
cake(5per
cent)
1
2
3
4
5
6
7
8
9
10
11

Harvesting(gm)
I
II

Treatment
Paddy
straw
+
wheat
bran(10per
cent)
1
2
3
4
5
6
7
8
9
10

Harvesting(gm)
I
II

360
365
350
370
390
350
320
340
355
360
375

III

Total
Cost
yield(g/500g (Rs.)
of
dry
substrate

70
580
70
590
90
600
80
610
80
620
80
610
90
590
70
580
70
615
90
680
75
610
Total
6685
Paddy straw supplemented with 5per cent cotton seed
6685 kg yield having cost Rs.1604.4

330
370
380
360
350
375
360
360
340
320

150
155
160
160
150
180
180
170
190
130
160

III

160
150
130
140
160
135
170
180
170
190

80
60
90
80
90
80
70
60
80
70
Total
20

139.2
141.6
144.0
146.4
148.8
146.4
141.6
139.2
147.6
163.2
146.4
1604.4
cake gives

Total
Cost
yield(g/500g (Rs.)
of
dry
substrate
570
580
600
580
600
590
600
600
590
580
5890

136.8
139.2
144.0
139.2
144.0
141.6
144.0
144.0
141.0
139.2
1413
Paddy straw supplemented with 10per cent wheat bran gives
5890 kg yield having cost Rs.1413

.
Treatment
Paddy
straw
+
Gram dal
flour(5per
cent)
1
2
3
4
5

Harvesting
I
II

255
260
265
270
275

118
135
130
125
130

Total
Cost
yield(g/500g (Rs.)
of
dry
substrate

III

72
60
55
70
60
Total

445
455
450
465
465
2280

106
109
108
111
111
545

Paddy straw supplemented with 5per cent gram dal flour gives
2280 kg yield having cost Rs.545

Treatment
Harvesting
Control
I
II
(unsupplement)
1
2
3
4
5

230
235
230
235
225

115
120
110
125
125

III
50
45
45
40
45
total

Total
yield(g/500g
of
dry
substrate
395
400
385
390
395
1965

Cost
(Rs.)
94
96
92
93
94
469

Paddy straw with no supplemented gives 1965 kg yield having
cost Rs.469

21
PRODUCTION COST


Cost required for spawn preparation Rs.240/-



Cost required for preparation of 80 beds is Rs.271/-



Supplements required for preparation of 80 beds is

Rs. 79.75/

Plastic bags required for marketing of mushrooms

Rs.8/

For preparation of one bed is Rs.13.50/-

Total input cost required for mushroom cultivation is
Rs.612.25

22
MARKETING STRATEGY OF MUSHROOM
We sold fresh mushroom in our college campus including
staff, student, worker, and also nearly people in village named
kharawate.
We also sold our mushroom to various in sawarda.

23
PROBLEM FACED
1.

Temperature and humidity

Due to high temperature there is slow growth of mycelium
on beds and due to this time required for harvesting increases.
Due to high temperature and low humidity ,the pinheads
and mature mushroom dries quickly.

24
SUGGESTION TO OVERCOME PROBLEM
1. For maintansing temperature and humidity in cropping room:
We had sprayed water on walls of the room.
We staged the water in room.
Wetted gunny bags were spread on the floors.
2. For collection of good quality paddy straw:
We collected the paddy straw from the nearest village “ Agave

25
BUDGET OF PROJECT

A) Total working capital
B) Depreciation on machinery and equipments at 10%
C) Interest on total capital investment at11%

A) Total working capital :1.Personal:
1. Manager
2. Supervisor
3. labor
Total

Rate
2500
1500
1000

Total (Rs)
30000
18000
12000
60000

2. Raw material:
1. Spawn
2. Paddy straw
3. Polyethylene
bag
4. Supplement
(wheat straw)
5. Cotton seed
cake
6. bamboo
Total
3. Utilities:
1. power
2. water
Total

Rate
90/kg
0.50/bag
0.50/bag

Quantity
200
5000
2200

Total (Rs)
18000
2500
1100

28/kg

18

504

30/kg

25

750

15/bamboo

20

300
23154

500/month
50/month

2000
600
2600

26
4.Other expenses:1.
2.
3.
4.
5.
6.

Land and building
Telephone
Transportation
Advertisement
Miscellaneous
Stationary
Total

6000/3000/12000/600/500/300/22400/-

Total working capital: 60000+23154+2600+22400=108154
B) Depreciation
Machinery
1. Laminar air
flaw
2. Autoclave
3. Freeze
4. Cooker
5. Cylinder
6. Sprayer
Total

on machinery:
Quantity
1

Rate (Rs)
35000

1
1
1
1
3

Total (Rs)
35000
15000
15000
500
2500
900
68900(10%)=6890

B) Depreciation on machinery =6890
C) Interest on total capital : 108154+68900=177054 (11%)=19475
D) Total project cost :-177054
E) Total income= 321600
F) B.C.R.= 321600/177054=1.8

27
Cost of production of mushroom for 80 beds :A. Variable
cost
Particular
Paddy straw
Bags

Unit/quantity

Rate

Amount (Rs)

160 bundle

0.50

80

80bag

1/bag

80

Other
Supplement
Labor charges

50
2 kg

28/kg

56

4

120/day

480

Interest on
working capital
(10%)
Rental value of
lab
Total variable
cost
B) Fixed cost

74.6
450
1196

Depreciation on
fixed capital
(rack)
Interest on fixed
capital @10%
C) Total fixed
cost
D) Total cost C

50
3
53
1249

E) Gross
returns
F) Net returns

6000
4751

G) B.C.R.

4.8

Conclusion : It is clear from above table the net returns required after
production of 80 beds of mushroom is Rs. 4751 and BCR is 4.8
28
CONCLUSION
 In Konkan region, the temperature is suitable for oyster mushroom
cultivation.
 Due it simple cultivation techniques it is very easy to cultivate
mushroom at home scale for farmers.
 In Konkan region, maximum farmers are depending on monsoon, so
after Kharif season there is no source of income for farmer. Oyster
mushroom cultivation gives big source of income to farmer due to its
easy method of cultivation, low production cost, high demand its
delicious taste, nutritional value and better market price.
 In Konkan region, there is easily availability of paddy straw and other
inputs that required for cultivation oyster mushroom cultivation.
 We used different supplement and thus found that there was yield by
using cotton seed cake as supplement.
 Due to delicious taste ,nutritional value and big demand we get better
market price.

29
REPORT OF EXPOSURE VISITE

Venue

:- College of Agriculture, Dapoli.

Date

:- 04 ⁄ 03 ⁄ 2012

We visited mushroom unit of College of Agriculture, Dapoli. We
meet the Prof. P. G. Borker. He was shown different types and species
of mushroom with it’s own research on power point presentation. He
was given the detail information about cultivation of White Button
Mushroom. He had shown morphology of mushroom and difference
between morphology of different species of mushroom. Borker Sir. Also
shown actual cultivation of Oyster Mushroom with frogger system.

30
FUTURE LINE OF WORK
Mushrooms are being used as a food and medicine since long time. Their
cultivation on extensive scale can help to solve many problems such as
protein shortage, resource recovery and reuse as well as part of
environmental management. it s necessary to cultivate it on large scale for
benefit of the country. Thus, it is necessary to fulfill the requirement of
enormous increasing population and hence alternate resource is production

31
References
Abere S.A and H.O Stanley (2011) cultivation of oyster mushroom
(Pleurotus tuber-regium on supplemented corn cob with rice bran of
substrates) Greener Journal of agriculture science vol. 1(2),pp.018021.

Chang S.T, O. W. Lao and K. Y. Chock (1981). The cultivation and nutritive
value of P. sajar caju. European J. Appl. Microbial. Biotechnol.,12:5862.

Chiejina, V. Nveka, olufokunbi and J. Olumide (2010). Effect on different
substrates on the yield and protein content of Pleurotus
tuberregium. Affrican J. of biotechnology vol 9(11) PP.1573-1577.

Indira. V., P. Dhasarathan and N.Anandadevi (2010). Impact of Agnihotra
in mushroom cultivation Technology. J. Biosci. Res.,2010 Vol 1(4)
:245-250.

Islam M. Z, M.H Rahman and F. Hafiz (2000). Cultivation of Oyster
mushroom (P.flabellatus) on different substates, In sustain crop
product. 4(1):45-48

Mandeel . A., A. A. Altaith and S.A. Mohame (2005). Cultivation on oyster
mushroom (Pleurotus spp.) on various lignocellulosic wastes. World
journal of microbiology and biotechnology 21(4):601-607.

32
ABBREVIATIONS

@

At the rate

◦C

Degree centigrade

et.al.

and other

g

Gram(s)

lit

Liter(s)

kg

Kilogram(s)

%

Percent

I.e.

That is

33
Isolation of pure culture

Pure culture of Oyster mushroom

Preparation of spawn

Isolation of pure culture

Figure 1

Preparation of spawn

Fully prepared spawn

Cutting of paddy straw
34
Soaking in cold water

Drained out excess cold water

Drain out excess hot water

Hot water treatment for sterilization

Preparation of Beds

Layer spawning

35
Pinning of mushroom bed

Spawn run

Spawn run period

Removal of polythene bag

Hanging of mushroom bed

Spraying of water on bed

36
Pin head stage

Mature mushroom

Spraying water on
mushroom bed
Harvesting of mushroom

Figure 2

Cleaning of mushroom

Harvested mushroom

Scrapping of mushroom bed

37
Mushroom for marketing

Mushroom pulav

Mushroom-pea curry

Mushroom omlet

Mushroom cutlet

38
39
40
41
Pin head stage

Mature mushroom

Harvesting of mushroom

Harvested mushroom

Cleaning of mushroom

Scrapping of mushroom bed

42
43
44
45

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Mushroom print2

  • 1. INTRODUCTION Mushroom cultivation is the most suitable technology for creating wealth out of plant, animal and industrial waste. Which are abundantly available all over the globe. Mushroom cultivation is the gaining momentum worldwide because of dietary food protein. Mushroom cultivation is unique in the sense that it is the most efficient and economically valuable technology for conservation of lignocelluloses material into high quality protein food. Mushroom cultivation can subsidies income of millions of farmers. Oyster mushroom is known as the wood fungus and in India is commonly known as “Dhingri”. Oyster mushroom is scientifically known as Pleurotus spp. Pleurotus is Basidiomycetes fungus belonging to order Agaricles and family Trichomataceae/Polyporaceae. There are about 38 species described under genus Pleurotus from different part of the world, out of which 25 species are under cultivation. They mainly include Pleurotus osteatus, Pleurotus eryngii, Pleurotus flabellatus, Pleurotus sajar caju, Pleurotus sapidus, Pleurotus membranaceous, Pleurotus citrinopileatus, Pleurotus eous and Pleurotus florida, P. cornicorpae. Pleurotus sajar caju was first reported by Jandaik and Kapoor in 1974, can be grown in the temperature range of 20-300C with 70-85per cent humidity, it has a good biological efficiency 84-90per cent under optimum condition. Oyster mushroom contains 90per cent moisture , 4-5per cent carbohydrate on wet weight basis, 20-35per cent protein, low sugar, low fat, low starch, high fibre and sodium potassium ratio. They contain good amount of C and B complex (thiamine, riboflavin and niacin). It also 1
  • 2. contains minerals, iron (Fe), Phosphorus (P), Potassium(K) and sodium(Na) and also low but available form of Iron(Fe). The Oyster mushroom Pleurotus spp. is saprophytic fungus commercially cultivated throughout the world because of its tasty basidiocarp and simple cultivation technology. Oyster mushroom is having capacity to grow in tropical and subtropical region. It is being cultivated in Himachal Pradesh, Uttar Pradesh, Andhra Pradesh, Jammu and Kashmir, Karnataka, Goa, Tamil Nadu and Panjab (Chandra and Sharma in 1995). Oyster mushroom can be use in different kitchen items such as pickle, chutney, pakoda, bhaji, pulav, mix vegetables, kofta and other delicious items etc. Its cultivation is popular due to its rapid mycelial growth, pleasant flavour, wide adoptablity and simple method of cultivation. Objectives:1) To learn various production techniques of mushroom production. 2) To get practical and experimental oriented knowledge of mushroom production. 3) To increases the yield of Pleurotus sajar caju by supplementation of different supplements. 4) To prepared value added products from fresh mushroom. 2
  • 3. REVIEW Mandeel et.al (2005). Cultivated three spp. of Pleurotus viz., P. columbinus, P.sajar-caju and P. ostreatus on organic waste such as chopped paper,cardboard, sawdust and plant fibres. Higher yield of P. columbinus was obtained on cardboard (135.5 g) followed by paper (100.8 g). P. ostreatus produced maximum yield on paper (112.4 g) however, P. sajar-caju failed to lowest yield on unsupplemented paddy straw (B.E 70 per cent). Islam et.al.(2009).Evaluated performance of Pleurotus flabellatus on seven different types of substrates viz., Mango, Jackfruit, Coconut, Jam, Kadom, Manogony, Shiris sawdust with wheat bran and CaCO3. The maximum biological efficiency was obtained with mango saw dust (150gm), followed by Monogony (148gm), Shiris (146gm), Kadom (136gm), Jam(114gm), Jackfruit (97gm) and Coconut saw dust (83gm). Coconut saw dust proved to be the poorest substrate for fruiting of this mushroom.produced satisfactory yield on all the substrates under study. Indira et.al (2010). Studied the effect of Agnihotra on yield of P. sajor-caju. In this experiment paddy straw was supplemented with Agnihotra ash ,Groundnut seed cake, Horse gram powder, Cotton seed powder among this supplement Agnihotra ash recorded higher (B.E. 97.5 per cent) yield in paddy straw while. Chiejinas et.al (2010). Studied the effect of seven substrates for cultivation of Pleurotus tuberregium. The substrates used were top soil, river sand, Fermented sawdust, Fermented oil palm fruit fiber, Mixture of top soil and Fermented sawdust, mixture of fermented oil palm fruit fibre and fermented sawdust, mixture of river sand and fermented sawdust. Among the seven substrates river sand and sawdust mixture produced significantly more yield. 3
  • 4. Abere and Stanley (2011). Cultivated P.Tuber-regium on corncob supplemented with rice bran at 10, 20 and 30 percent concentration on dry weight basis. 30per cent supplementation of rice bran gave higher yield as compared to other treatments. 4
  • 5. LOCATION i. ii. Name of Department:- Department of Plant Pathology Name of College :- Sharadchandraji Pawar College of Agriculture, Kharwate-Dahiwali. Tal.- Chiplun, Dist.- Ratnagiri. iii. Name of University :- Dr. Balasaheb Sawant Konkan Krishi Vidhyapeeth, Dapoli. WORKING PERIOD 1 December 2011 to 4 April 2012 5
  • 6. INPUT DETAILS: (Quantity and Cost of input) A. For preparation of spawn (For 80 beds): Sr.No Inputs Quantity 1 2 3 4 5 6 Wheat grain CaCO3 powder Gas Electricity Cotton bags Cost (Rs.) 5kg 400gm 2 units 90 bags 115 100 5 8 10 2 Total 240 Cost required for preparation 8 kg spawn is Rs.240. B. Cost required for preparation of one bed: Sr.No Inputs Quantity 1 2 3 4 5 500gm 25gm 1 - 0.50 0.50 0.50 2 1 100gm 9 Total 6 Paddy straw Supplements Bags Labour cost Other(Detol, rubber,Pin) Spawn Cost (Rs.) 13.50 For preparation one bed Rs. 13.50 Required C. Cost required for preparation of 80 beds: Sr.No Inputs Quantity 1 2 3 4 5 40 80 1 bottle 1 packet 40 40 160 24 7 Total 271 Paddy straw Bags Labour cost Detol Rubber Cost required for preparation of 80 beds is Rs.271 6 Cost (Rs.)
  • 7. D. Supplement required for preparation of 50 beds: Sr.No Supplement Quantity (g) Cost (Rs.) 1 3 4 500 9 250 15 250 6 - - Total 2 Wheat bran(10 per cent) Gram dal flour(5 per cent) Cotton seed cake(5 per cent) Groundnut seed cake(5 per cent) 30 Cost required for supplementation of 25 beds is Rs. 30 E. For marketing of mushroom bags required: Total cost of bags- Rs. 8 F. Input Sr.No 1 2 3 4 used for preparation of mushroom pakoda Items Quantity (g) Cost (Rs.) Mushroom 125 30 Gram flour 100 7 Baking According to 2 powder,salt,turmeric taste powder, red chilli oil 250 10 total 47 Cost required for preparation of mushroom pakoda is Rs.47 7
  • 8. G. Input used for preparation of mushroom pulav Sr.No Items Quantity (g) 1 2 3 4 5 6 7 8 9 10 Mushroom Rice Onion Cumin seed Cardamom Bay leaf Clove Black pepper Ghee salt 125 100 1 ½tables spoon 2 2 2 2 2 tables spoon According to taste total Cost (Rs.) 30 3 1 9 9 1 53 Cost required for preparation of mushroom pulav is Rs.53 H. Total input cost required for mushroom cultivation Sr.No Items Cost 1 2 3 4 5 Spawn preparation Preparation of 80 beds Supplements of 25 beds Dehydration of mushrooms Packaging bags total Total input Rs.486.75/- cost required 8 for 106 271 30 30 486.75 mushroom cultivation is
  • 9. SUMMARY OF WORK PERFORMANCE Mushroom is ideal agent for conversion of agro waste into protein rich material. Among all the Pleurotus spp. is having ability to grow in temperature range of 20-300C, its substrate colonization time is late and it is more delicious than most of the species. Oyster mushroom was grown on paddy straw. Paddy straw are easily available in Konkan region, it act as good substrate for mushroom cultivation. So that paddy straw was used for cultivation of Pleurotus sajar caju. Mushroom beds were treated by using paddy straw substrate supplemented with different supplements such as, wheat bran (10per cent on dry weight basis substrate), cotton seed cake, groundnut seed cake, gram dal flour (5 per cent on dry weight basis of substrate). Among the substrate supplement combination tied highest yield was observed in paddy straw supplemented with cotton seed cake (610 g/500g dry substrate) that followed by paddy straw supplemented with wheat bran (600 g). However lowest yield was observed in controlled treatment (Unsupplemented paddy straw). From fresh mushroom we prepared value added products such as mushrooms pakoras, mushroom pulav, mushroom pickle, mushroom bhaji. 9
  • 10. FLOWCHART Steps involve in preparation of spawn Good quality wheat grains Clean and dry wheat grains Soaked in water for (8-10 hrs) Boiled the wheat grains up to they become soft(Avoid splitting) Remove excess water Spread on plastic sheet and add 4% CaCo3 Fill in conical flasks Plugged with non-absorbent cotton Kept in autoclave at 15 lbs pressure at 121◦C for 15 min. Remove it from autoclave Inoculation with pure culture Incubate at room temperature (25±20C) for 1 week Commercial spawn 10
  • 11. Steps involve in preparation of Mushroom bed Raw material (paddy straw) Chopping (3-5 cm) Fill up in gunny bags Soaking in water (8-12 hrs) Drain off excess water Dip in hot water (80-85◦C) for 1-2 hrs Drain of excess water On cooling to room temperature bags with filled in layers of substrate, supplement and spawn Spawn running (15-20 days) Remove plastic bags cover and maintain humidity, light and ventilation Pinhead stage (3-5 days) 11
  • 12. Cropping fruit flush, (3-4 days duration after pinhead) and subsequent flushes about 10 days interval Picking on maturity (before spore shedding start) Marketing Fresh/dried 12
  • 13. MUSHROOM PAKODA Fresh mushroom (125gm) Cleaning and washing with water Cut into transverse pieces Mix them with gram flour, salt, turmeric powder, red chilli powder and baking soda Add little water Heat oil Fry mushroom pakodas till it become light brown 13
  • 14. MUSHROOM PULAV Good quality mushroom (125g) Cleaning, washing and cutting in small pieces Fry lightly in ghee and kept aside Add cumin, cardamom, cloves, black pepper and bay leaves in ghee Add onion and fry upto light brown colouration Add rice and double quantity water and salt to taste Add mushrooms after boiling of rice Cook on slow fire for few minute Serve in plates 14
  • 15. MUSHROOM CUTLET Fresh good quality of mushroom (125gm) Clean and cut them into small pieces Boiled the peeled green peas and potato make it into a paste Cut the onion, green chillies, coriander leave into small pieces] Grind the ginger into paste Mixed all the mixture and add turmeric powder, chili powder and salt Make the mixture into ball Dip the ball in gram flour paste Fry it in oil Serve in plates 15
  • 16. METHOD OF PRODUCTION Maintenance of culture: The pure culture of Pleurotus sajar caju was obtained from the Department of Plant Pathology, College of Agriculture,Dapoli. The culture was maintained on potato dextrose agar medium and sub cultured at regular interval of three weeks. Preparation of spawn Spawn of pleurotus sajor caju was prepared on wheat grains. The grains were cleaned and boiled in hot water for 20-30 minutes taking care that the grains did not burst. After boiling, these grains were spread on filter paper for removing the excess water. Calcium carbonate@ 3-4per cent (wet weight basis) was mixed uniformly with grains and then filled into 500 ml milk bottles (each bottle contain 300gm boiled wheat grains). The bottles were plugged with non absorbent cotton and sterilized in an autoclave at 1.54kg/cm2 pressure for one hour. The bottles were inoculated with equal mycelial bits obtained from pure culture and incubated at room temperature (27±1◦C) for 10-15 days. Preparation of substrate Paddy straw is common waste farm in Konkan region also it obtained in low cost. Paddy straw contains high percentage of cellulose so that it was use as a substrate for cultivation of Pleurotus sajor caju. Paddy straw used for cultivation was stiff, clean and undamaged. The substrates was first chopped into 2”-3” pieces then filled in gunny bags and soaked overnight in cold water. 500 gm dry substrate was used for preparing one mushroom bed. After overnight soaking weight of 500 gm dry substrate increases to 2 kg wet substrate. Therefore one bed comprised of 500 gm dry substrate i.e.2 kg wet substrate. The gunny bag congaing soaked substrate was then transferred to plastic 16
  • 17. drum with lid. Then hot boiling water was poured in the drum is such a way that the substrate was thoroughly in hot water. The lid was then placed on the drum to facilitate pasteurization. Preparation of supplements: Flour protein rich supplements viz., wheat bran, cotton seed cake, groundnut seed cake and gram dal flour were used for increasing the yield and increasing biological efficiency of Pleurotus sajor caju. Among the flour supplements, wheat bran was used at 10per cent flour of dry weight of substrate while cotton seed cake, gram dal flour and groundnut seed cake were used at 5per cent level. Prior to use, required quantities of the supplements were sterilized by autoclaving. Supplements were added to substrates at the time of spawning. Bed filling and maintenance After preparation of substrates and supplements by the above procedure, mushroom beds were prepared by using appropriate quantity of each substrate with each supplement. Two kg wet substrate can be properly accommodated in plastic bag (100 gauges) of size 55X35 cm. Hence these bags were used for filling beds. The beds were prepared by filling the layers of substrate, supplements and spawn in a sequence. Rate of spawning was two percent on wet weight basis of substrate. Filled plastic bag were then perforated on all side to provide proper aeration. Darkness favours rapid colonization of the substrate. Therefore these plastic bags were kept in the darkness at room temperature. Substrate colonization period range between 15-20 days depending upon the temperature and substrates supplement combination. 17
  • 18. When the substrate was fully colonize, beds were opened and hanged to a firm support. Beds were watered as and required by giving gentle sprayer of water. Humidity in cropping rooms was maintained between 82-85 per cent. After first harvest, each bed was trimmed on the all side by a sharp knife. By maintaining 80-85per cent humidity, second flush was taken within a period of 10-12 and third flush 10 days after the second. Observation on biological efficiency were recorded by the formula, Biological efficiency = Fresh weight of mushroom X 100 Air dried substrates (Chang et.al, 1981) Different recopies of mushroom Mushroom pakoras Mushroom -125g Gram dal flour -100g Backing powder - a pinch Salt, turmeric powder, chilli powder-According to test Oil to fry -250g First take the 300 g mushroom then clean, washed and cut into transverse pieces. Mushroom pieces were mixed with gram dal flour, salt, turmeric powder, red chilli powder and baking soda as per required amount with little water for preparation of butter. After that oil was heated and fried mushroom pakoras at a time till they become brown. 18
  • 19. Mushroom pulav Mushroom -125gm Rice -100g Onion -1 Cumin seed - Cardamom -1 Bay leaf -2 Clove -1 to 2 Black pepper -2 to3 Ghee -2 table spoon Salt -according to taste table spoon The good quality of mushroom were selected for preparation of pulav. Mushroom were cleaned and washed with water and cut into small pieces. Then mushroom were fried in ghee. Afterfrying kept it aside. In some ghee add cumin, coriander, cardamom, clove, black pepper and bay leaves. When cumin seed where being to crackled onion was added and tied till light brown color appeared then rice were added with double quantity of water and salt for taste. After boiling of rice, fried mushroom pieces were added in rice. Then cooked on slow fire till the pulao was ready. 19
  • 20. QUANTITY OF PRODUCE Treatment Paddy straw + cotton seed cake(5per cent) 1 2 3 4 5 6 7 8 9 10 11 Harvesting(gm) I II Treatment Paddy straw + wheat bran(10per cent) 1 2 3 4 5 6 7 8 9 10 Harvesting(gm) I II 360 365 350 370 390 350 320 340 355 360 375 III Total Cost yield(g/500g (Rs.) of dry substrate 70 580 70 590 90 600 80 610 80 620 80 610 90 590 70 580 70 615 90 680 75 610 Total 6685 Paddy straw supplemented with 5per cent cotton seed 6685 kg yield having cost Rs.1604.4 330 370 380 360 350 375 360 360 340 320 150 155 160 160 150 180 180 170 190 130 160 III 160 150 130 140 160 135 170 180 170 190 80 60 90 80 90 80 70 60 80 70 Total 20 139.2 141.6 144.0 146.4 148.8 146.4 141.6 139.2 147.6 163.2 146.4 1604.4 cake gives Total Cost yield(g/500g (Rs.) of dry substrate 570 580 600 580 600 590 600 600 590 580 5890 136.8 139.2 144.0 139.2 144.0 141.6 144.0 144.0 141.0 139.2 1413
  • 21. Paddy straw supplemented with 10per cent wheat bran gives 5890 kg yield having cost Rs.1413 . Treatment Paddy straw + Gram dal flour(5per cent) 1 2 3 4 5 Harvesting I II 255 260 265 270 275 118 135 130 125 130 Total Cost yield(g/500g (Rs.) of dry substrate III 72 60 55 70 60 Total 445 455 450 465 465 2280 106 109 108 111 111 545 Paddy straw supplemented with 5per cent gram dal flour gives 2280 kg yield having cost Rs.545 Treatment Harvesting Control I II (unsupplement) 1 2 3 4 5 230 235 230 235 225 115 120 110 125 125 III 50 45 45 40 45 total Total yield(g/500g of dry substrate 395 400 385 390 395 1965 Cost (Rs.) 94 96 92 93 94 469 Paddy straw with no supplemented gives 1965 kg yield having cost Rs.469 21
  • 22. PRODUCTION COST  Cost required for spawn preparation Rs.240/-  Cost required for preparation of 80 beds is Rs.271/-  Supplements required for preparation of 80 beds is Rs. 79.75/ Plastic bags required for marketing of mushrooms Rs.8/ For preparation of one bed is Rs.13.50/- Total input cost required for mushroom cultivation is Rs.612.25 22
  • 23. MARKETING STRATEGY OF MUSHROOM We sold fresh mushroom in our college campus including staff, student, worker, and also nearly people in village named kharawate. We also sold our mushroom to various in sawarda. 23
  • 24. PROBLEM FACED 1. Temperature and humidity Due to high temperature there is slow growth of mycelium on beds and due to this time required for harvesting increases. Due to high temperature and low humidity ,the pinheads and mature mushroom dries quickly. 24
  • 25. SUGGESTION TO OVERCOME PROBLEM 1. For maintansing temperature and humidity in cropping room: We had sprayed water on walls of the room. We staged the water in room. Wetted gunny bags were spread on the floors. 2. For collection of good quality paddy straw: We collected the paddy straw from the nearest village “ Agave 25
  • 26. BUDGET OF PROJECT A) Total working capital B) Depreciation on machinery and equipments at 10% C) Interest on total capital investment at11% A) Total working capital :1.Personal: 1. Manager 2. Supervisor 3. labor Total Rate 2500 1500 1000 Total (Rs) 30000 18000 12000 60000 2. Raw material: 1. Spawn 2. Paddy straw 3. Polyethylene bag 4. Supplement (wheat straw) 5. Cotton seed cake 6. bamboo Total 3. Utilities: 1. power 2. water Total Rate 90/kg 0.50/bag 0.50/bag Quantity 200 5000 2200 Total (Rs) 18000 2500 1100 28/kg 18 504 30/kg 25 750 15/bamboo 20 300 23154 500/month 50/month 2000 600 2600 26
  • 27. 4.Other expenses:1. 2. 3. 4. 5. 6. Land and building Telephone Transportation Advertisement Miscellaneous Stationary Total 6000/3000/12000/600/500/300/22400/- Total working capital: 60000+23154+2600+22400=108154 B) Depreciation Machinery 1. Laminar air flaw 2. Autoclave 3. Freeze 4. Cooker 5. Cylinder 6. Sprayer Total on machinery: Quantity 1 Rate (Rs) 35000 1 1 1 1 3 Total (Rs) 35000 15000 15000 500 2500 900 68900(10%)=6890 B) Depreciation on machinery =6890 C) Interest on total capital : 108154+68900=177054 (11%)=19475 D) Total project cost :-177054 E) Total income= 321600 F) B.C.R.= 321600/177054=1.8 27
  • 28. Cost of production of mushroom for 80 beds :A. Variable cost Particular Paddy straw Bags Unit/quantity Rate Amount (Rs) 160 bundle 0.50 80 80bag 1/bag 80 Other Supplement Labor charges 50 2 kg 28/kg 56 4 120/day 480 Interest on working capital (10%) Rental value of lab Total variable cost B) Fixed cost 74.6 450 1196 Depreciation on fixed capital (rack) Interest on fixed capital @10% C) Total fixed cost D) Total cost C 50 3 53 1249 E) Gross returns F) Net returns 6000 4751 G) B.C.R. 4.8 Conclusion : It is clear from above table the net returns required after production of 80 beds of mushroom is Rs. 4751 and BCR is 4.8 28
  • 29. CONCLUSION  In Konkan region, the temperature is suitable for oyster mushroom cultivation.  Due it simple cultivation techniques it is very easy to cultivate mushroom at home scale for farmers.  In Konkan region, maximum farmers are depending on monsoon, so after Kharif season there is no source of income for farmer. Oyster mushroom cultivation gives big source of income to farmer due to its easy method of cultivation, low production cost, high demand its delicious taste, nutritional value and better market price.  In Konkan region, there is easily availability of paddy straw and other inputs that required for cultivation oyster mushroom cultivation.  We used different supplement and thus found that there was yield by using cotton seed cake as supplement.  Due to delicious taste ,nutritional value and big demand we get better market price. 29
  • 30. REPORT OF EXPOSURE VISITE Venue :- College of Agriculture, Dapoli. Date :- 04 ⁄ 03 ⁄ 2012 We visited mushroom unit of College of Agriculture, Dapoli. We meet the Prof. P. G. Borker. He was shown different types and species of mushroom with it’s own research on power point presentation. He was given the detail information about cultivation of White Button Mushroom. He had shown morphology of mushroom and difference between morphology of different species of mushroom. Borker Sir. Also shown actual cultivation of Oyster Mushroom with frogger system. 30
  • 31. FUTURE LINE OF WORK Mushrooms are being used as a food and medicine since long time. Their cultivation on extensive scale can help to solve many problems such as protein shortage, resource recovery and reuse as well as part of environmental management. it s necessary to cultivate it on large scale for benefit of the country. Thus, it is necessary to fulfill the requirement of enormous increasing population and hence alternate resource is production 31
  • 32. References Abere S.A and H.O Stanley (2011) cultivation of oyster mushroom (Pleurotus tuber-regium on supplemented corn cob with rice bran of substrates) Greener Journal of agriculture science vol. 1(2),pp.018021. Chang S.T, O. W. Lao and K. Y. Chock (1981). The cultivation and nutritive value of P. sajar caju. European J. Appl. Microbial. Biotechnol.,12:5862. Chiejina, V. Nveka, olufokunbi and J. Olumide (2010). Effect on different substrates on the yield and protein content of Pleurotus tuberregium. Affrican J. of biotechnology vol 9(11) PP.1573-1577. Indira. V., P. Dhasarathan and N.Anandadevi (2010). Impact of Agnihotra in mushroom cultivation Technology. J. Biosci. Res.,2010 Vol 1(4) :245-250. Islam M. Z, M.H Rahman and F. Hafiz (2000). Cultivation of Oyster mushroom (P.flabellatus) on different substates, In sustain crop product. 4(1):45-48 Mandeel . A., A. A. Altaith and S.A. Mohame (2005). Cultivation on oyster mushroom (Pleurotus spp.) on various lignocellulosic wastes. World journal of microbiology and biotechnology 21(4):601-607. 32
  • 33. ABBREVIATIONS @ At the rate ◦C Degree centigrade et.al. and other g Gram(s) lit Liter(s) kg Kilogram(s) % Percent I.e. That is 33
  • 34. Isolation of pure culture Pure culture of Oyster mushroom Preparation of spawn Isolation of pure culture Figure 1 Preparation of spawn Fully prepared spawn Cutting of paddy straw 34
  • 35. Soaking in cold water Drained out excess cold water Drain out excess hot water Hot water treatment for sterilization Preparation of Beds Layer spawning 35
  • 36. Pinning of mushroom bed Spawn run Spawn run period Removal of polythene bag Hanging of mushroom bed Spraying of water on bed 36
  • 37. Pin head stage Mature mushroom Spraying water on mushroom bed Harvesting of mushroom Figure 2 Cleaning of mushroom Harvested mushroom Scrapping of mushroom bed 37
  • 38. Mushroom for marketing Mushroom pulav Mushroom-pea curry Mushroom omlet Mushroom cutlet 38
  • 39. 39
  • 40. 40
  • 41. 41
  • 42. Pin head stage Mature mushroom Harvesting of mushroom Harvested mushroom Cleaning of mushroom Scrapping of mushroom bed 42
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  • 44. 44
  • 45. 45