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同一産卵巣内で孵化した
 アカウミガメ幼体の
 血中テストステロン濃度と孵化温度
 の関係
   The relation of the serum concentration of
 testosterone and incubation temperature in the
 loggerhead turtle (Caretta caretta) hatching from the
                      same nest
○八田しおり¹、齋藤純康²、岡田勇治²、勝俣悦子²、唐木伊助³、渡辺元¹、田谷一善¹

  ¹東京農工大学獣医生理学研究室、
  ²鴨川シーワールド、³セイコーエプソン株式会社CS品質保証環境推進部
Introduction
Introduction
アカウミガメ
―Caretta caretta (Loggerhead turtle)
―IUCN Red List : Endangered

―5-8月産卵
―産卵巣:直径20cm×深さ60cm
―70-150個×1-5回/年(隔年)
Introduction
千葉県鴨川市
―鴨川シーワールド
  平成14年~ウミガメ保護活動
   6-8月:保護
   8-9月:孵化
Introduction
温度依存性性決定機構
        29.4℃以上   雌が多い      (T:低 E2:高)
―Temperature-dependent
        Sex Determination
―臨界点:29.4℃
 29.4℃以上 雌が多い (T:低 E2:高)
 29.4℃       雄:雌=1:1 (臨界点)
 29.4℃以下 雄が多い (T:高 E2:低)
       29.4℃以下 雄が多い (T:高 E2:低)


性判定法
 Teststerone(T):男性ホルモン
 Estradiol (E2):女性ホルモン
Objective
Objective
同一巣内の性比分布

1. 同一巣内の深度毎の経時的温度測定
    上層:30cm/中層:40cm/下層:50cm

2. 同一巣内から孵化した幼体の
    深度別血中テストステロン濃度測定

3. 光量の産卵巣への影響
Materials and
  Methods
Materials and Methods
Materials and Methods

  30cm
  40cm
  50cm
※精度±0.125℃
Materials and Methods
採血
―場所 :背側頚洞
―注射器:1.0mlシリンジ、26G注射針
―量  : 0.3-0.5ml

ラジオイムノアッセイ(RIA)法
 ―血漿中テストステロン濃度
Results
Results

                自然光巣                遮光巣

産卵日(保護日)   2011年7月3日(7月18日)   2011年6月20日(6月25日)

産卵場所       東条海岸               東条海岸
           鴨川グランドホテル前         鴨川グランドホテル前

掘り起し       9月1日(初回脱出8月30日)    9月1日、5日


n数         51個体               129個体
           (上:24、中:23、下:4)    (上:44、中:43、下:42)
51個体
Results:自然光巣   (上:24、中:23、下:4)
Results:遮光巣   129個体
              (上:44、中:43、下:42)
Results:平均温度
Results:テストステロン濃度

          上:1個体(2.3%)
          中:9個体(20.9%)
          下:7個体(16.7%)
             :T値超高濃度個体
             (>1000pg/ml)
          0個体(0%)
Summary
Summary
1. 同一産卵巣内の卵でも
    深い場所ほど周囲温度が低く、

2. 同一産卵巣内でも
    深い場所で孵化した幼体ほど
     血漿中テストステロン濃度は高い

3. 太陽光を遮ると
    砂中温度は2-3℃下がり、
     テストステロン濃度も高くなる
Thank you for your attention !!

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野生動物医学会2012 o 13, hatta