1. Photosynthesis:
The Calvin Cycle
Life from Air
AP Biology
2007-2008

2. Whoops! Wrong Calvin…
The Calvin Cycle
AP Biology
1950s | 1961

3. Remember what it means to be a plant…
 Need to produce all organic molecules
necessary for growth

carbohydrates, lipids, proteins, nucleic acids
 Need to store chemical energy (ATP)
produced from light reactions
in a more stable form
 that can be moved around plant
 saved for a rainy day

carbon + water + energy → glucose + oxygen
dioxide
light → C H O + 6O
AP Biology6CO2 + 6H2O +
6 12 6
2
energy

4. Light reactions
 Convert solar energy to chemical
energy
ATP

ATP → energy

NADPH → reducing power
 What can we do now?
→ → build stuff !!
AP Biology
photosynthesis

5. How is that helpful?
 Want to make C6H12O6
synthesis
 How? From what?
What raw materials are available?

CO2
NADPH
carbon fixation
reduces CO2
NADP
C6H12O6
AP Biology
NADP

6. From CO2 → C6H12O6
 CO2 has very little chemical energy

fully oxidized
 C6H12O6 contains a lot of chemical energy

highly reduced
 Synthesis = endergonic process

put in a lot of energy
 Reduction of CO2 → C6H12O6 proceeds in
many small uphill steps
each catalyzed by a specific enzyme
 using energy stored in ATP & NADPH

AP Biology

7. From Light reactions to Calvin cycle
 Calvin cycle

chloroplast stroma
 Need products of light reactions to
drive synthesis reactions
ATP
 NADPH

ATP
thylakoid
AP Biology
stroma

8. C
Calvin cycle
1C
C C C C C
C C C C C
3. Regeneration
C C C C C
of RuBP
RuBP
starch,
sucrose,
cellulose
& more
ribulose bisphosphate
3 ATP
H H H
|
| |
C–C–C
AP Biology
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
C
CO2
1. Carbon fixation
C C C C C C
RuBisCo
ribulose
bisphosphate
carboxylase
3 ADP
used
to make
glucose
C=C=C
5C
C
C
6C
C C C C C C
C C C C C C
5C
glyceraldehyde-3-P
G3P
3C
C C C
phosphoglycerate
6 NADP
3C
C
C
C
C
C
C
6 ATP
2. Reduction
6 NADPH
PGA
C
C
C
C
C
C
3C
6 ADP
C
C
C
C
C
C
H
|
H
|
H
|

9. Remember
G3P?
glycolysis
glucose
C-C-C-C-C-C
2 ATP
2 ADP
fructose-1,6bP
P-C-C-C-C-C-C-P
DHAP
P-C-C-C
G3P
glyceraldehyde
3-phosphate
C-C-C-P
2 NAD+
2
4 ADP
AP Biology
Photosynthesis
pyruvate
C-C-C
4 ATP

10. To G3P and Beyond!
 Glyceraldehyde-3-P
To G3P
and beyond!
end product of Calvin cycle
 energy rich 3 carbon sugar
 “C3 photosynthesis”

 G3P is an important intermediate
 G3P → → glucose → → carbohydrates
→ → lipids → → phospholipids, fats, waxes
→ → amino acids → → proteins
→ → nucleic acids → → DNA, RNA
AP Biology

11. RuBisCo
 Enzyme which fixes carbon from air
ribulose bisphosphate carboxylase
 the most important enzyme in the world!

 it makes life out of air!

definitely the most abundant enzyme
I’m green
with envy!
AP Biology
It’s not easy
being green!

12. Accounting
 The accounting is complicated

3 turns of Calvin cycle = 1 G3P

3 CO2 → 1 G3P (3C)

6 turns of Calvin cycle = 1 C6H12O6 (6C)

6 CO2 → 1 C6H12O6 (6C)

18 ATP + 12 NADPH → 1 C6H12O6

AP Biology
any ATP left over from light reactions
will be used elsewhere by the cell

13. Photosynthesis summary
 Light reactions
produced ATP
 produced NADPH
 consumed H O
2
 produced O as byproduct
2

 Calvin cycle
consumed CO2
 produced G3P (sugar)
 regenerated ADP
 regenerated NADP

AP Biology
ADP
NADP

14. Light Reactions
light → ATP + NADPH + O
2
energy
H 2O +
H2O
sunlight
Energy Building
Reactions
NADPH
ATP
AP Biology
O2
 produces ATP
 produces NADPH
 releases O2 as a
waste product

15. Calvin Cycle
CO2 + ATP + NADPH → C6H12O6 + ADP + NADP
CO2
ADP
NADP
Sugar
Building
Reactions
NADPH
ATP
AP Biology
sugars
 builds sugars
 uses ATP &
NADPH
 recycles ADP
& NADP
 back to make
more ATP &
NADPH

16. Putting it all together
light
CO2 + H2O + energy → C6H12O6 + O2
H2O
CO2
sunlight
ADP
Energy NADP
Building
Reactions
Sugar
Building
Reactions
NADPH
ATP
AP Biology
O2
sugars
Plants make both:
 energy
 ATP & NADPH
 sugars

17. even though
this equation
is a bit of a lie…
it makes a
better story
Energy cycle
sun
Photosynthesis
light
CO2 + H2O + energy → C6H12O6 + O2
plants
CO2
H 2O
animals, plants
glucose
ATP
C6H12O6 + O2 → energy + CO2 + H2O
Cellular Respiration
AP Biology
The Great Circle
of Life,Mufasa!
ATP
O2

18. Summary of photosynthesis
6CO2 + 6H2O + light → C6H12O6 + 6O2
energy
 Where did the CO2 come from?
 Where did the CO2 go?
 Where did the H2O come from?
 Where did the H2O go?
 Where did the energy come from?
 What’s the energy used for?
 What will the C6H12O6 be used for?
 Where did the O2 come from?
 Where will the O2 go?
 What else is involved…not listed in this equation?
AP Biology

19. Supporting a biosphere
 On global scale,
photosynthesis is the
most important process
for the continuation of life on Earth

each year photosynthesis…
 captures 121 billion tons of CO2
 synthesizes 160 billion tons of carbohydrate

AP Biology
heterotrophs are dependent on plants as
food source for fuel & raw materials

20. The poetic perspective…
 All the solid material of every plant

was built by sunlight out of thin air
All the solid material of every animal
was built from plant material
air
AP Biology
sun
Then all the plants, cats,
dogs, elephants & people …
are really particles of air woven
together by strands of sunlight!

21. If plants can do it…
You can learn it!
Ask Questions!!
AP Biology
2007-2008