Presented by Roxana Hickey (PhD candidate, University of Idaho) at the University of Oregon META Symposium on "Host-Microbe Systems Biology: Modeling Our Microbial Selves" 8-10 Aug 2014 in Eugene, OR
Community dynamics of the adolescent vaginal microbiome during puberty (UOregon META Symposium 2014)
1. Community dynamics of the
adolescent vaginal
microbiome during puberty
Roxana Hickey
University of Idaho
META Symposium on Host-Microbe Systems Biology
9 August 2014
@roxana_hickey
2. How does the vaginal microbiome
change over a lifespan?
3. Birth
Vagina sterile in utero (?), then transiently colonized by
mother’s vagina or skin microbiota upon delivery
9. Vaginal microbiota differ among individuals and
are often variable over short time scales
Ravel et al. (2011) PNAS
Gajer et al. (2012) Sci Transl Med
11. Vaginal microbiota of postmenarcheal adolescents (13-18y)
closely resemble those of reproductive age women
Yamamoto et al. (2009) J Pediatr Adolesc Gynecol
Thoma et al. (2011) J Pediatr Adolesc Gynecol
12. What is the timing and sequence of events that occur as
vaginal microbiota transition to an ‘adult-like’ state?
13. Study design
microbiota composition
๏ 16S rRNA V1-V3 pyrosequencing
๏ mothur + RDP Naïve Bayesian
Classifier
24 mothers
sampled annually
31 girls enrolled
@ age 10-12 &
premenarcheal
sampled every 3 months for up to 3 years
21 girls became
postmenarcheal
during study
metadata
๏ pubertal development (Tanner stage)
๏ menarcheal status
๏ 21 Black
๏ 8 Caucasian
๏ 2 Native American
26. Same,
●
●
●●
●
●
●
●
●
●
●
−5
0
5
2 3 4 5
Tanner breast
Logit−transformedLABproportion
a
●
●●
−5
0
5
pre post
Menarche status
●
4
5
6
7
2 3 4 5
Tanner breast
VaginalpH
b
4
5
6
7
pre post
Menarche status
!
but different
28. Acknowledgements
Procter & Gamble
FEMCARE DIVISION
Melanie Hansmann
Kristin Malone
Julie Erb
Lisa Bowman
Funding
Procter & Gamble
NIH P30 RR033376
UI BCB Graduate Fellowship
Indiana University
SCHOOL OF MEDICINE
J. Dennis Fortenberry
Alexis Roth
Cynthia Robbins
Marcia Shew
SCHOOL OF PUBLIC HEALTH
Barbara van der Pol
University of Idaho
Larry Forney
Xia Zhou
Matt Settles
Dorah Mtui
Daniel Beck
Matt Pennell
Sam Hunter
Forney & Top Labs
IBEST