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Ramadass Balamurugan

GLUTAMINE ATTENUATES THE TOXIC EFFECTS OF LEGUMES VIA STIMULATION OF HSP PRODUCTION Ramadass B , Dokladny K, Moseley P and Lin H C New Mexico Veterans Affairs Health systems and the University of New Mexico, Albuquerque, NM

Background ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Toxicity of Raw Red Kidney Beans ,[object Object],[object Object]

Glutamine in Critical Illness ,[object Object],[object Object],[object Object],[object Object],[object Object]

Reported Effects of Glutamine ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Heat Shock Proteins ,[object Object],[object Object],[object Object]

Methods ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Methods ,[object Object],[object Object],[object Object],[object Object]

Experimental Outcomes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Results Glutamine Reduced RRKB-induced Leaky Gut ,[object Object],RRKB vs Glutamine+RRKB, P<0.001

Glutamine Reduced RRKB-induced Bacterial Translocation to the Liver. RRKB vs Glutamine+RRKB, P<0.001

Glutamine Increased HSP expression in Mid and Distal Small Intestine Glutamine vs Glutamine+RRKB, P<0.05

HSP70 was still available on Day 8 ,[object Object],[object Object],[object Object],[object Object],Control & RRKB groups Glutamine & Glutamine+RRKB groups

PHA Decreased TER in CaCO-2 cells In-vitro .

PHA Decreased Proteins Responsible for Intestinal Barrier Function

Doubly-expressing HSP70 Cells are Protected from Lectin-induced Decrease in Protein Folding

Summary/Interpretation ,[object Object],[object Object],[object Object]

Conclusions ,[object Object],[object Object]

Future Studies ,[object Object],[object Object]

Acknowledgment ,[object Object],[object Object]

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1. GLUTAMINE ATTENUATES THE TOXIC EFFECTS OF LEGUMES VIA STIMULATION OF HSP PRODUCTION Ramadass B , Dokladny K, Moseley P and Lin H C New Mexico Veterans Affairs Health systems and the University of New Mexico, Albuquerque, NM

10.

11.

12. Glutamine Reduced RRKB-induced Bacterial Translocation to the Liver. RRKB vs Glutamine+RRKB, P<0.001

13. Glutamine Increased HSP expression in Mid and Distal Small Intestine Glutamine vs Glutamine+RRKB, P<0.05

14.

15.

16. PHA Decreased TER in CaCO-2 cells In-vitro .

17. PHA Decreased Proteins Responsible for Intestinal Barrier Function

18.

19. Doubly-expressing HSP70 Cells are Protected from Lectin-induced Decrease in Protein Folding

20.

21.

22.

23.

24.

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