CELL FRACTIONATION AND ITS STEPS

1. LE 6-5A
Homogenization
Homogenate
Tissue
cells
Differential centrifugation

2. • Tissue is cut into pieces and placed in cold isotonic
buffer solution.
• Pieces of tissue are ground into very small fragments
in a type of liquidiser called as homogeniser.
• The suspension of cell fragments is filtered to remove
large pieces of tissue debris
• The filterate is then placed in a centrifuge