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Staphylococcus  and Related Organisms  Staphylococcus Micrococcu . www.freelivedoctor.com
Preliminary Grouping of Gram Positive Cocci ,[object Object],Catalase Salt Tolerant Yes No Facultative Yes No Rothia Staphylococcus Micrococcus Streptococcus Group + _ www.freelivedoctor.com
General characteristics ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
General characteristics ,[object Object],[object Object],[object Object],www.freelivedoctor.com
General characteristics ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Staph clusters Micrococcus  tetrads Rothia www.freelivedoctor.com
General characteristics ,[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
General characteristics ,[object Object],[object Object],[object Object],www.freelivedoctor.com
General Clinical Significance ,[object Object],[object Object],[object Object],www.freelivedoctor.com
General Clinical Significance ,[object Object],[object Object],www.freelivedoctor.com
General Clinical Significance not on test ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Coagulase positive Coagulase negative www.freelivedoctor.com
General Clinical Significance ,[object Object],[object Object],[object Object],www.freelivedoctor.com
General Growth Characteristics ,[object Object],[object Object],[object Object],www.freelivedoctor.com
General Growth Characteristics ,[object Object],[object Object],[object Object],www.freelivedoctor.com
General Cultural Characteristics ,[object Object],[object Object],[object Object],www.freelivedoctor.com
General Cultural Characteristics ,[object Object],[object Object],[object Object],www.freelivedoctor.com
General Cultural Characteristics ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
General Cultural Characteristics ,[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Biochemical characteristic ,[object Object],[object Object],www.freelivedoctor.com
Biochemical characteristic ,[object Object],[object Object],www.freelivedoctor.com
Presumptive Genus Identification table not on test   6.5%  Furazoli-  Modified  0.04 U   Salt  done  oxidase  Bacitracin Micrococcus  +  R   +    S Staphylococcus  + S   -   R R. mucilaginosa   - NT  NT   NT Not included: oxygen requirements www.freelivedoctor.com
Coagulase ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Coagulase Staphylococcus aureus  coagulation of plasma*   “ Free” staphylocoagulase  Fibrinogen + CRF  Fibrin Normal coagulation of plasma       Thrombin Fibrinogen  Fibrin (soluble )   (insoluble)  *EDTA Rabbit plasma is preferred for the “free” or tube coagulase test because it contains a large amount of CRF www.freelivedoctor.com
Coagulase testing ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Coagulase testing ,[object Object],[object Object],www.freelivedoctor.com
Coagulase testing ,[object Object],[object Object],www.freelivedoctor.com
Coagulase testing ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Coagulase testing ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Coagulase testing ,[object Object],[object Object],www.freelivedoctor.com
Clumping Factor ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Clumping Factor ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Clumping Factor ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Clumping Factor ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Protein A ,[object Object],[object Object],www.freelivedoctor.com
Protein A clumping test ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Protein A Latex Test S S S S S S L L L L L IgG S= S.aureus  with Protein A L=Latex particle Protein A S Fc S www.freelivedoctor.com
Protein A/Clumping factor ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Other  S. aureus  Characteristics ,[object Object],[object Object],www.freelivedoctor.com
Other  S. aureus  Characteristics ,[object Object],[object Object],[object Object],www.freelivedoctor.com
S .  aureus  diagnosis - summary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Pathology - predisposing factors ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
S. aureus  is a problem ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Upper respiratory tract  ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Lower respiratory tract ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Lower respiratory tract ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Integument / wounds ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Integument / wounds ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Food poisoning ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
S. aureus  – other pathology  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Virulence Factors of  S. aureus ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Virulence Factors of  S. aureus ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Virulence Factors of  S. aureus ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
VF: more enzymes / toxins ,[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Antimicrobial Susceptibility ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Antimicrobial Susceptibility   ,[object Object],[object Object],www.freelivedoctor.com
Antimicrobial Susceptibility ,[object Object],[object Object],www.freelivedoctor.com
Co agulase  N egative  S taphylococcus ,[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
CoNS –  S. epidermidis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
CoNS –  S. epidermidis ,[object Object],[object Object],www.freelivedoctor.com
CoNS –  S. saprophyticus ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Coagulase Negative  Staphylococcus ,[object Object],[object Object],www.freelivedoctor.com
Micrococcus  species ,[object Object],[object Object],[object Object],www.freelivedoctor.com
Micrococcus  species ,[object Object],[object Object],www.freelivedoctor.com
www.freelivedoctor.com
Rothia  mucilaginosus ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],www.freelivedoctor.com
Presumptive Genus Identification table not on test   6.5%  Furazoli-  Modified  0.04 U   Salt  done  oxidase  Bacitracin Micrococcus  +  R   +    S Staphylococcus  + S   -   R R. mucilaginosa   - NT  NT   NT www.freelivedoctor.com

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Mdl 237 Staphylococci

  • 1. Staphylococcus and Related Organisms Staphylococcus Micrococcu . www.freelivedoctor.com
  • 2.
  • 3.
  • 4.
  • 5.
  • 6. Staph clusters Micrococcus tetrads Rothia www.freelivedoctor.com
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  • 12.
  • 13.
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  • 19.
  • 20.
  • 21. Presumptive Genus Identification table not on test 6.5% Furazoli- Modified 0.04 U Salt done oxidase Bacitracin Micrococcus + R + S Staphylococcus + S - R R. mucilaginosa - NT NT NT Not included: oxygen requirements www.freelivedoctor.com
  • 22.
  • 23. Coagulase Staphylococcus aureus coagulation of plasma* “ Free” staphylocoagulase Fibrinogen + CRF Fibrin Normal coagulation of plasma Thrombin Fibrinogen Fibrin (soluble ) (insoluble) *EDTA Rabbit plasma is preferred for the “free” or tube coagulase test because it contains a large amount of CRF www.freelivedoctor.com
  • 24.
  • 25.
  • 26.
  • 27.
  • 28.
  • 29.
  • 30.
  • 31.
  • 32.
  • 33.
  • 34.
  • 35.
  • 36. Protein A Latex Test S S S S S S L L L L L IgG S= S.aureus with Protein A L=Latex particle Protein A S Fc S www.freelivedoctor.com
  • 37.
  • 38.
  • 39.
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  • 63.
  • 65.
  • 66. Presumptive Genus Identification table not on test 6.5% Furazoli- Modified 0.04 U Salt done oxidase Bacitracin Micrococcus + R + S Staphylococcus + S - R R. mucilaginosa - NT NT NT www.freelivedoctor.com

Notas do Editor

  1. Staphylococci
  2. Staphylococci