25. Bone marrow – mediated endogenous
‘pro-regenerative’ resposne after AMI
26. Bone marrow – mediated endogenous
‘pro-regenerative’ resposne after AMI
SDF expression in infarct zone
27. Myocardial regeneration therapy in 2014+
Optimal cell type
and dose ?
Jagiellonian University Dept. of Cardiac & Vascular Diseases
John Paul II Hospital
Krakow
31. 10 years
Jagiellonian University Dept. of Cardiac & Vascular Diseases
John Paul II Hospital
Krakow
+
32.
33.
34.
35.
36.
37.
38.
39.
40.
41.
42.
43.
44.
45.
46. Myocardial regeneration therapy in 2014+
How to (best)
deliver the cells?
Jagiellonian University Dept. of Cardiac & Vascular Diseases
John Paul II Hospital
Krakow
47. DEVIC
E
Development
Optimized
retention
Homing signals
Cell functionality
Cell adhesion
Matrices
Survival
TISSU
E
BIOLOGIC
S
Injection method & technique
Needle design
Injection speed
Tissue-device compatibility
Biocompatibility
Cell concentration
Viscosity
Stability
Adapted from J. Bartunek et al. Clinical Pharm & Ther 85, 548-552
48.
49.
50.
51.
52.
53.
54.
55. Labeled cells (SPECT) and cMRI: I M A G E F U S I O N
P Banyś 2010-2012
Obrazowanie hybrydowe:
zawał (MRI) / komórki (SPECT)
Labeled CD34+ cells
(3D SPECT)
myocardial
CD34+ uptake
(part of) CD34+ liver uptake
68. • CE Mark since April 2012
• Available on the market
69. Development
Needle design based on mathematical modeling
Variable caliber and distribution of infusion holes: Drug Product
is more evenly distributed along the needle extremity
Enhanced fluid distribution inside myocardium
76. Critical Limb Ischaemia
variable (naturally) disease progression rate
...
concomittant revasculatization of the feeding vessel(other confounders (such as exercise – proangiogenic)
77.
78.
79.
80.
81.
82. INVESTIGATOR – INITIATED STUDY
Novel Off-shelf
Regenerative Cell Application in Treatment
of No-option Critical Limb Ischemia
Mariusz TRYSTUŁA, Marcin MAJKA (PIs)
email: mrt@onet.pl
Jagiellonian University Dept. of Cardiac & Vascular Surgery
and Transplantation at John Paul II Hospital
and Jagiellonian University Dept. of Transplantation
Krakow
