1. 2010/04/22
kaneko.satoko(at)ocha.ac.jp

2. 10

3.
PC

4.
(1):

5. 1953 DNA
1966 ( )
1972
1975‐7
1985 PCR
1986
1993
1995 DNA
1997 E.coli ( ) (4.6Mb)
2000
2003 (3.3Gb)

6. 1972
1975‐7 (Sanger Maxam‐Gilbert )
1985 PCR (6kb = 6000bp/day)
1986 DNA
1993
2000 (600kb = 600,000bp/day)
2007 Microarray like sequence (600Mb = 600,000,000bp/day)
2010 ? (100Gb = 100,000,000,000bp/hour)
(3.3Gb) 4

7. 1971 MEDLINE
1980 DNA
EMBL(European Molecular Biology Laboratory)
1982 DNA GenBank
1985 FASTA
1986 Swiss‐Prot
1988 NCBI(Na[onal Center for Biotechnology Informa[on)
Human Genome Ini[a[ve Bioinforma[cs
CLUSTAL
1990 BLAST
1991 World Wide Web
2000 Ensembl , 2001 UCSC

8. 1991 Linda B. Buck and Richard Axel (The Nobel Prize in Physiology or Medicine for 2004)
G primer
PCR (a) (b)
(c) (18 loci)
(a)
(b)
(c)
(from & )
1992 1998

9.
1999 Richard Axel John Carlson
2007 Drosophila 12 Genomes Consor[um
Drosophila melanogaster( ) 11
12
(812 loci)
(from )

10. (1)
ATGC
35
1946 (DNA )
8/20=40%
16/19=84%

11. DNA
(2):
‐ (omics)‐

12. 1958 Francis Crick
(DNA)
DNA RNA
RNA
replica[on transcrip[on transla[on
( )
( )
( )
reverse transcrip[on
( )
splicing
( )
RNA (non‐coding RNA)

13. (2):
exon1
exon2
exon3
exon4
exon5
exon6
5'
3'
intron1
intron2
intron5
intron3
intron4
exon: ( )
intron: exon( ) exon
5' UTR: 5' mRNA ( )
3' UTR: 3' mRNA ( )
coding sequence (CDS): ( )
( )
1) exon1 exon6 mRNA (transcrip[on)
2) exon ( splicing )
3) (transla[on)
alterna[ve splicing: exon splicing
exon
exon1+2+3+4+5+6 exon1+2+5+6 exon1+2+3+6

14. ‐ (omics)‐
(omics)
(genome)
(DNA)
(transcpritome)
(mRNA)
gene + ome
transcript + ome
omics
(proteome)
( )
(metabolome)
(
)
(phenome)
( )

15.
DNA GenBank( ) EMBL( ) DDBJ( ) UCSC( )
(SNPs) dbSNP( ) JSNP( )
OMIM( ) Muta[on database( )
SWISS‐PROT( ) PIR( )
Pfam( )
PROSITE( ) BLOCKS( )
PDB( ) SCOP( ) CATH( )
KEGG( )
MEDLINE( )
NCBI( ) Ensembl( )

16. PC
Mac OS
MacBook Pro – ‐
Dock
gcc (Xcode)
X11
CotEditor
MacPorts
Unarchiver

17. Mac OS
Mac
OS

18. (1)

19. (2)
(
10~20 )

20. Mac OS ( )
Mac
OS

21. Finder
Finder Macintosh HD
Finder
Finder
:
:
:

22. Mac
Mac OS X dock
dock
dock
Finder
Safari
(
)
Safari

23. (1)
Machintosh HD/ /Symantec Solu[ons Live Update

24. (2)
Symantec Scheduler

25. (3)
Mac
Symantec An[Virus 10.1.1 for Macintosh

26. (4)

27. (5)
3

28. (1)
Machintosh HD/ /Symantec Solu[ons Symantec An[virus
Macintosh HD

29. (2)
Symantec An[Virus
Symantec An[Virus

30. MacBook Pro – ‐
Dock
Dock
Finder

31. Dock
Macintosh HD/ /
Dock (
Dock )

32. gcc (Xcode) (1)
MacBook Pro
MacBook Pro Mac OS X Install Disc 1
MacBook Pro Mac OS X Install DVD
Xcode (IDE)
gcc (GNU Compiler Collec[on)
gcc C C++ Java

33. gcc (Xcode) (2‐1)
Op[onal Installs → Xcode Tools → Xcode Tools.mpkg
Xcode Tools.mpkg

34. gcc (Xcode) (2‐2)
( MacBook Pro )
→ Xcode Tools → Xcode Tools
Xcode Tools

35. gcc (Xcode) (3‐1)

36. gcc (Xcode) (3‐2)
( MacBook Pro )

37. gcc (Xcode) (4)

38. X11 (1‐1)
MacBook Pro Mac OS X Install Disc 1
(Xcode
)
Op[onal Installs → Op[onal Installs.mpkg
X11 MacOSX X Window System
X Window System XWS UNIX GUI
X

39. X11 (1‐2)
( MacBook Pro )
MacBook Pro Mac OS X Install Disc 1
(Xcode
)
→ Op[onal Installs
X11 MacOSX X Window System
X Window System XWS UNIX GUI
X

40. X11 (2)

41. X11 (3‐1)
X11
*

42. X11 (3‐2)
hop://xquartz.macosforge.org/
XQuartz 2.5.0 released ‐> Download X11‐2.5.0.dmg (Leopard)

43. X11 (4)

44. (CotEditor) (1)
(CotEditor)
5
(2009 5 1 CotEditor 1 0 1)

45. (CotEditor) (2)
101
CotEditor

46. MacPorts (1)
UNIX
MacPorts
(2010 4 19 MacPorts‐1.8.2)

47. MacPorts (2)

48. MacPorts (3)
PATH
$echo $PATH
[enter return ]
$
/opt/local/bin
(:)
PATH
$ export PATH=$PATH:/opt/local/bin
( page , page )
$echo $PATH
PATH

49. MacPorts (4)
port
/opt/local/bin/ /opt/local/bin/
OS
*

50. (1)
tg04

51. (2)
Unarchiver
8

52. PC
‐
‐
UNIX (NCBI Ensembl)
viewer