SlideShare uma empresa Scribd logo
1 de 15
Baixar para ler offline
A
                   SEMINAR ON

Downstream processing
      of vaccine
          (e.g. Influenza & Tetanus)



              Presented By-
           NIYAMAT M. PANJESHA
               SNEHA SAWLE
              YUGENDRA PATIL


  Modern College Of Arts , Science & Commerce.
             Shivajinagar, Pune-05.
What are Vaccines?
• A vaccine is a biological preparation that improves
  immunity to a particular disease.
• Name derived from the word vacca which means cow.
• Can be- 1) Prophylactic. 2) Therapeutic.

           Edward Jenner observed that the farmers and milkmaids working with cows
                 developed a mild form of small pox called cowpox or vaccinia.

            The cowpox infection could protect these people from infection of smallpox.


                   It was in 1796 Jenner experimentally tested this observation.

                          He inoculated an 8 yr old boy with exudates from a cowpox
                          lesion and he repeated it twice with a gap of some weaks.

                              Jenner noticed that smallpox did not develop in this boy.
                                  This was 1st discovery of principle of vaccination.
Live attenuated
                           Organism
                                    Killed
         Traditional

                                       Toxoid

                         Sub-unit
Types     Recombinant             Conjugate


                                             Vector
                         DNA
        Experimental    vaccine
                               Edible
                              vaccine
Examples of Vaccines
Sr.   Vaccine                     Organism                   Disease
No.
1     Live Attenuated Bacterial   •Bacillus anthracis        •Aanthrax
      Vaccine                     •Salmonella typhii         •Typhoid


2     Inactivated Bacterial
      Vaccines
      Capsular Ag                •Neissseria meningitides   •Meningitis
      Cell wall Ag               •Vibrio Cholera            •Cholera
      Toxoid Ag                  •Clostridium tetani        •Tetanus

3     Recombinant Vaccines
      Bacterial                  •Clostridium tetani        •Tetanus
      Protozoal                  •Plasmodium vivax          •Malaria
      Viral                      -H1N1                      •Influenza

4     DNA vaccines                                           •Malaria
Steps In Vaccine
  Production
processing
 SELECTING THE STRAIN FOR




                                                   Upstream
 VACCINE PRODUCTION

              GROWING THE MICRO-ORGANISM


                ISOLATION & PURIFICATION OF
                MICROORGANISM
Downstream
 processing




                   INACTIVATION OF ORGANISM


                     FORMULATION OF VACCINE


                        QUALITY CONTROL AND LOT RELEASE
Influenza Vaccine
Two modes: Seasonal Epidemic and
Pandemic Influenza


Types of Vaccine
Production:
1.Egg based(Traditional)
2.Cell line based


Three forms of vaccine:
A.Whole
B.Split
C.Subunit/Surface Antigen
Live Virus Vaccine
   Production using
  MDCK cell line on
Cytodex3 micro carrier
  Upstream Process:

0.5X106 cells/ml(at 100r.p.m.
          for 48hrs.)
 At 2-2.5X106 cells/ml infect
with Viral inoculum and carry
         out for 24hr.
DOWNSTREAM
         PROCESSING
1.Clarification:
ULTA Prime GF capsule
Filtration(0.6µm)

2.Diafiltration(0.4µm) in
buffer[20mM Tris+0.5M NaCl,
pH7.5]

3.DNA binding Anion Exchange
Chromatography: To remove gDNA

4.Sterile Filtration(0.2µm):
Stainless steel filter precoated with
buffer.
Tetanus is an acute and highly fatal disease caused by
exotoxins produced by the bacterium Clostridium.tetani.

Cl. tetani produces two exotoxins- tetanolysin and
tetanospasmin.

Tetanus toxoid is one of the most immunogenic
antigens available for the protection against an infectious
disease in the world.

Tetanus Vaccine produced by anaerobic fermentation.
Tetanus Vaccine Production

                          Revival of the       Preparation of seed
 Production strain
                        production strain            culture




   Production of
  tetanus toxin by                              Determination of
                        Harvesting of Toxin
   Static Culture                               antigenic content
      Method




Detoxification of the   Partial purification      Purification of
        Toxin            of tetanus toxin      crude tetanus toxoid
                                                      



Production strain       Inoculation        Seed culture       Fermentation         Purification




                                                                                      
Monosodium                               Formaldehyde       Glycine       
 glutamate                Alum                 soln              soln


   Stabilizer           Adjuvant                               Dilutant
                                           Inactivation                          Ultrafilteration
      




  Thimersol
                                                                       


 Preservative                               Inspection        Labeling            Packaging
                             Filling
                                                                                           12
Dsp of vaccine
REFERENCES
Nielsen, P. A.1967. Large-Scale Production of Vaccine. Applied Microbiology.

Ozutsumi K, Sugimoto N, and Matsuda M. 1985. Rapid, Simplified Method for
Production and Purification of tetanus toxin. Applied and environmental
microbiology.

 Chandani Payal and Tejpal Kashyap, 2011. Production, Optimization of
Detoxification and Ammonium Sulphate Precipitation of Ultrafiltered Tetanus
Toxin. Recent Research in Science and Technology, 3(11): 49-54.

 Nduka Okafor, Modern industrial microbiology and biotechnology.

U.Satyanarayan, A Text book of Biotechnology.

Michael J.Waites, Industrial Microbiology: An Introduction.

GE Healthcare Life Sciences, Application note 28-9843-41 AA Vaccines
Propagation and purification of influenza virus.
QUESTIONS, CRITICISMS, AND
      SUGGESTIONS?




                                 THANK YOU



         snehasawle@rediffmail.com
        niyamatpanjesha@yahoo.com

Mais conteúdo relacionado

Mais procurados

Mais procurados (20)

SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINE
SYNTHETIC PEPTIDE  VACCINES  AND RECOMBINANT  ANTIGEN VACCINESYNTHETIC PEPTIDE  VACCINES  AND RECOMBINANT  ANTIGEN VACCINE
SYNTHETIC PEPTIDE VACCINES AND RECOMBINANT ANTIGEN VACCINE
 
Recombinant Vaccines
Recombinant VaccinesRecombinant Vaccines
Recombinant Vaccines
 
Recombinant protein-Factor VIII
Recombinant protein-Factor VIIIRecombinant protein-Factor VIII
Recombinant protein-Factor VIII
 
Interferons
InterferonsInterferons
Interferons
 
interferon
interferoninterferon
interferon
 
Upstream processing
Upstream processingUpstream processing
Upstream processing
 
Vaccine preparation part 2
Vaccine preparation part 2Vaccine preparation part 2
Vaccine preparation part 2
 
DNA vaccines (types, method and mechanism)
DNA vaccines (types, method and mechanism)  DNA vaccines (types, method and mechanism)
DNA vaccines (types, method and mechanism)
 
Introduction to vaccines
Introduction to vaccinesIntroduction to vaccines
Introduction to vaccines
 
Viral vaccines
Viral vaccinesViral vaccines
Viral vaccines
 
Plantibodies
PlantibodiesPlantibodies
Plantibodies
 
Insulin production
Insulin productionInsulin production
Insulin production
 
Hybridoma Technology
Hybridoma TechnologyHybridoma Technology
Hybridoma Technology
 
Gene manipulation
Gene manipulationGene manipulation
Gene manipulation
 
Bt brinjal controversy
Bt brinjal controversyBt brinjal controversy
Bt brinjal controversy
 
DNA Vaccine
DNA VaccineDNA Vaccine
DNA Vaccine
 
Recombinant production of insulin
Recombinant production of insulinRecombinant production of insulin
Recombinant production of insulin
 
Vaccine production
Vaccine productionVaccine production
Vaccine production
 
Application of rDNA technology to produce Interferon, Hepatitis-B Vaccine & I...
Application of rDNA technology to produce Interferon, Hepatitis-B Vaccine & I...Application of rDNA technology to produce Interferon, Hepatitis-B Vaccine & I...
Application of rDNA technology to produce Interferon, Hepatitis-B Vaccine & I...
 
Production of Recombinant Pharmaceuticals
Production of Recombinant PharmaceuticalsProduction of Recombinant Pharmaceuticals
Production of Recombinant Pharmaceuticals
 

Destaque

Vaccine Production
Vaccine ProductionVaccine Production
Vaccine ProductionZainabSaif
 
Conventional methods of animal vaccine production
Conventional methods of animal vaccine productionConventional methods of animal vaccine production
Conventional methods of animal vaccine productionDr Vijayata choudhary
 
Vaccines –production and application
Vaccines –production and applicationVaccines –production and application
Vaccines –production and application2624
 
5. vaccine technology
5. vaccine technology5. vaccine technology
5. vaccine technologyBruno Mmassy
 
Dna vaccine final ppt
Dna vaccine final pptDna vaccine final ppt
Dna vaccine final pptTaye Desta
 
Vaccines,types,composition & failure etc
Vaccines,types,composition & failure etcVaccines,types,composition & failure etc
Vaccines,types,composition & failure etcDr. Waqas Nawaz
 
Vaccination ppt
Vaccination pptVaccination ppt
Vaccination pptali7070
 
EMPHNET-PHE course: Module03 ethical issues in surveillance, screening and ou...
EMPHNET-PHE course: Module03 ethical issues in surveillance, screening and ou...EMPHNET-PHE course: Module03 ethical issues in surveillance, screening and ou...
EMPHNET-PHE course: Module03 ethical issues in surveillance, screening and ou...Dr Ghaiath Hussein
 
Vaccine against viruses
Vaccine against virusesVaccine against viruses
Vaccine against virusesSadaqur Rahman
 
Immunization
ImmunizationImmunization
ImmunizationUbaid N P
 
Use of Plants as Bioreactors for edible vaccine production
Use of Plants as Bioreactors for edible vaccine productionUse of Plants as Bioreactors for edible vaccine production
Use of Plants as Bioreactors for edible vaccine productionEric Paul
 

Destaque (20)

Vaccines
VaccinesVaccines
Vaccines
 
Vaccine Production
Vaccine ProductionVaccine Production
Vaccine Production
 
Vaccine & Its Type
Vaccine & Its TypeVaccine & Its Type
Vaccine & Its Type
 
Vaccine
Vaccine Vaccine
Vaccine
 
Conventional methods of animal vaccine production
Conventional methods of animal vaccine productionConventional methods of animal vaccine production
Conventional methods of animal vaccine production
 
Vaccines –production and application
Vaccines –production and applicationVaccines –production and application
Vaccines –production and application
 
5. vaccine technology
5. vaccine technology5. vaccine technology
5. vaccine technology
 
Dna vaccine final ppt
Dna vaccine final pptDna vaccine final ppt
Dna vaccine final ppt
 
Vaccines,types,composition & failure etc
Vaccines,types,composition & failure etcVaccines,types,composition & failure etc
Vaccines,types,composition & failure etc
 
Vaccination ppt
Vaccination pptVaccination ppt
Vaccination ppt
 
EDIBLE VACCINES
EDIBLE VACCINESEDIBLE VACCINES
EDIBLE VACCINES
 
Tetanus t
Tetanus tTetanus t
Tetanus t
 
EMPHNET-PHE course: Module03 ethical issues in surveillance, screening and ou...
EMPHNET-PHE course: Module03 ethical issues in surveillance, screening and ou...EMPHNET-PHE course: Module03 ethical issues in surveillance, screening and ou...
EMPHNET-PHE course: Module03 ethical issues in surveillance, screening and ou...
 
Vaccine against viruses
Vaccine against virusesVaccine against viruses
Vaccine against viruses
 
Efficacy testing of the rVSV-ZEBOV Ebola vaccine in Guinea, Dr. Gunnstein No...
Efficacy testing of the rVSV-ZEBOV Ebola vaccine in Guinea, Dr. Gunnstein No...Efficacy testing of the rVSV-ZEBOV Ebola vaccine in Guinea, Dr. Gunnstein No...
Efficacy testing of the rVSV-ZEBOV Ebola vaccine in Guinea, Dr. Gunnstein No...
 
Tetanus
TetanusTetanus
Tetanus
 
Immunization
ImmunizationImmunization
Immunization
 
Tetanus (lock jaw)
Tetanus (lock jaw)Tetanus (lock jaw)
Tetanus (lock jaw)
 
Use of Plants as Bioreactors for edible vaccine production
Use of Plants as Bioreactors for edible vaccine productionUse of Plants as Bioreactors for edible vaccine production
Use of Plants as Bioreactors for edible vaccine production
 
Ph02 edible vaccines
Ph02 edible vaccinesPh02 edible vaccines
Ph02 edible vaccines
 

Semelhante a Dsp of vaccine

Downstream Processing of Tetanus Vaccine
Downstream Processing of Tetanus VaccineDownstream Processing of Tetanus Vaccine
Downstream Processing of Tetanus Vaccinekushal raj roy
 
Recombinant Proteins in Plants :Problems and Prospects
Recombinant Proteins in Plants :Problems and ProspectsRecombinant Proteins in Plants :Problems and Prospects
Recombinant Proteins in Plants :Problems and ProspectsSenthil Natesan
 
7 current advancement in parasite treatment
7 current advancement in parasite treatment7 current advancement in parasite treatment
7 current advancement in parasite treatmentIrwan Izzauddin
 
The Nature Of Disease Controlling Disease
The Nature Of Disease Controlling DiseaseThe Nature Of Disease Controlling Disease
The Nature Of Disease Controlling Diseasedoc_sawyer
 
Evidencias2006ii 100619025253-phpapp01
Evidencias2006ii 100619025253-phpapp01Evidencias2006ii 100619025253-phpapp01
Evidencias2006ii 100619025253-phpapp01UNICA-Publicidad
 
Anthrax ..
Anthrax ..Anthrax ..
Anthrax ..El_Omda
 
Principles of management of virus diseases
Principles of management of virus diseasesPrinciples of management of virus diseases
Principles of management of virus diseasesN.H. Shankar Reddy
 
The Nature Of Disease Controlling Disease
The Nature Of Disease Controlling DiseaseThe Nature Of Disease Controlling Disease
The Nature Of Disease Controlling Diseasedoc_sawyer
 
vaccination in fish
vaccination in fishvaccination in fish
vaccination in fish40024
 
Chapter 4 isolation identification-and-cultivation
Chapter 4 isolation identification-and-cultivationChapter 4 isolation identification-and-cultivation
Chapter 4 isolation identification-and-cultivationAlia Najiha
 
Lab dig virus
Lab dig virusLab dig virus
Lab dig virusPrbn Shah
 
Evidencias2006 ii
Evidencias2006 iiEvidencias2006 ii
Evidencias2006 iiHOME
 
recent development in culture od Cestode
recent development in culture od Cestoderecent development in culture od Cestode
recent development in culture od CestodeAbdullah Jan
 

Semelhante a Dsp of vaccine (20)

Downstream Processing of Tetanus Vaccine
Downstream Processing of Tetanus VaccineDownstream Processing of Tetanus Vaccine
Downstream Processing of Tetanus Vaccine
 
Lecture 4 sterilization
Lecture 4 sterilizationLecture 4 sterilization
Lecture 4 sterilization
 
Recombinant Proteins in Plants :Problems and Prospects
Recombinant Proteins in Plants :Problems and ProspectsRecombinant Proteins in Plants :Problems and Prospects
Recombinant Proteins in Plants :Problems and Prospects
 
7 current advancement in parasite treatment
7 current advancement in parasite treatment7 current advancement in parasite treatment
7 current advancement in parasite treatment
 
Bacillus anthracis
Bacillus anthracisBacillus anthracis
Bacillus anthracis
 
The Nature Of Disease Controlling Disease
The Nature Of Disease Controlling DiseaseThe Nature Of Disease Controlling Disease
The Nature Of Disease Controlling Disease
 
Applied virology converted
Applied virology convertedApplied virology converted
Applied virology converted
 
Enterococcus
EnterococcusEnterococcus
Enterococcus
 
Evidencias2006ii 100619025253-phpapp01
Evidencias2006ii 100619025253-phpapp01Evidencias2006ii 100619025253-phpapp01
Evidencias2006ii 100619025253-phpapp01
 
Anthrax ..
Anthrax ..Anthrax ..
Anthrax ..
 
Principles of management of virus diseases
Principles of management of virus diseasesPrinciples of management of virus diseases
Principles of management of virus diseases
 
The Nature Of Disease Controlling Disease
The Nature Of Disease Controlling DiseaseThe Nature Of Disease Controlling Disease
The Nature Of Disease Controlling Disease
 
VIROLOGY - CULTIVATION AND DETECTION OF VIRUS
VIROLOGY - CULTIVATION AND DETECTION OF VIRUSVIROLOGY - CULTIVATION AND DETECTION OF VIRUS
VIROLOGY - CULTIVATION AND DETECTION OF VIRUS
 
vaccination in fish
vaccination in fishvaccination in fish
vaccination in fish
 
Chapter 4 isolation identification-and-cultivation
Chapter 4 isolation identification-and-cultivationChapter 4 isolation identification-and-cultivation
Chapter 4 isolation identification-and-cultivation
 
Lab dig virus
Lab dig virusLab dig virus
Lab dig virus
 
Evidencias2006 ii
Evidencias2006 iiEvidencias2006 ii
Evidencias2006 ii
 
Vaccines
Vaccines Vaccines
Vaccines
 
recent development in culture od Cestode
recent development in culture od Cestoderecent development in culture od Cestode
recent development in culture od Cestode
 
Vaccine
VaccineVaccine
Vaccine
 

Último

blood bank management system project report
blood bank management system project reportblood bank management system project report
blood bank management system project reportNARMADAPETROLEUMGAS
 
Male Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondMale Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondSujoy Dasgupta
 
Basic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxBasic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxkomalt2001
 
Clinical Research Informatics Year-in-Review 2024
Clinical Research Informatics Year-in-Review 2024Clinical Research Informatics Year-in-Review 2024
Clinical Research Informatics Year-in-Review 2024Peter Embi
 
Moving Forward After Uterine Cancer Treatment: Surveillance Strategies, Testi...
Moving Forward After Uterine Cancer Treatment: Surveillance Strategies, Testi...Moving Forward After Uterine Cancer Treatment: Surveillance Strategies, Testi...
Moving Forward After Uterine Cancer Treatment: Surveillance Strategies, Testi...bkling
 
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfSGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfHongBiThi1
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.whalesdesign
 
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxBreast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxNaveenkumar267201
 
Female Reproductive Physiology Before Pregnancy
Female Reproductive Physiology Before PregnancyFemale Reproductive Physiology Before Pregnancy
Female Reproductive Physiology Before PregnancyMedicoseAcademics
 
High-Performance Thin-Layer Chromatography (HPTLC)
High-Performance Thin-Layer Chromatography (HPTLC)High-Performance Thin-Layer Chromatography (HPTLC)
High-Performance Thin-Layer Chromatography (HPTLC)kishan singh tomar
 
Adenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisAdenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisSujoy Dasgupta
 
Physiotherapy Management of Rheumatoid Arthritis
Physiotherapy Management of Rheumatoid ArthritisPhysiotherapy Management of Rheumatoid Arthritis
Physiotherapy Management of Rheumatoid ArthritisNilofarRasheed1
 
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdfSGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdfHongBiThi1
 
The Importance of Mental Health: Why is Mental Health Important?
The Importance of Mental Health: Why is Mental Health Important?The Importance of Mental Health: Why is Mental Health Important?
The Importance of Mental Health: Why is Mental Health Important?Ryan Addison
 
Mental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil ThirusanguMental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil Thirusangu Medical University
 
introduction to neurology (nervous system, areas, motor and sensory systems)
introduction to neurology (nervous system, areas, motor and sensory systems)introduction to neurology (nervous system, areas, motor and sensory systems)
introduction to neurology (nervous system, areas, motor and sensory systems)Mohamed Rizk Khodair
 
Microbiology lecture presentation-1.pptx
Microbiology lecture presentation-1.pptxMicrobiology lecture presentation-1.pptx
Microbiology lecture presentation-1.pptxkitati1
 
CPR.nursingoutlook.pdf , Bsc nursing student
CPR.nursingoutlook.pdf , Bsc nursing studentCPR.nursingoutlook.pdf , Bsc nursing student
CPR.nursingoutlook.pdf , Bsc nursing studentsaileshpanda05
 
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdfSGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdfHongBiThi1
 
How to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyHow to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyZurück zum Ursprung
 

Último (20)

blood bank management system project report
blood bank management system project reportblood bank management system project report
blood bank management system project report
 
Male Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and BeyondMale Infertility, Antioxidants and Beyond
Male Infertility, Antioxidants and Beyond
 
Basic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptxBasic structure of hair and hair growth cycle.pptx
Basic structure of hair and hair growth cycle.pptx
 
Clinical Research Informatics Year-in-Review 2024
Clinical Research Informatics Year-in-Review 2024Clinical Research Informatics Year-in-Review 2024
Clinical Research Informatics Year-in-Review 2024
 
Moving Forward After Uterine Cancer Treatment: Surveillance Strategies, Testi...
Moving Forward After Uterine Cancer Treatment: Surveillance Strategies, Testi...Moving Forward After Uterine Cancer Treatment: Surveillance Strategies, Testi...
Moving Forward After Uterine Cancer Treatment: Surveillance Strategies, Testi...
 
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdfSGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
SGK LEUKEMIA KINH DÒNG BẠCH CÂU HẠT HAY.pdf
 
MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.MedMatch: Your Health, Our Mission. Pitch deck.
MedMatch: Your Health, Our Mission. Pitch deck.
 
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptxBreast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
Breast cancer -ONCO IN MEDICAL AND SURGICAL NURSING.pptx
 
Female Reproductive Physiology Before Pregnancy
Female Reproductive Physiology Before PregnancyFemale Reproductive Physiology Before Pregnancy
Female Reproductive Physiology Before Pregnancy
 
High-Performance Thin-Layer Chromatography (HPTLC)
High-Performance Thin-Layer Chromatography (HPTLC)High-Performance Thin-Layer Chromatography (HPTLC)
High-Performance Thin-Layer Chromatography (HPTLC)
 
Adenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosisAdenomyosis or Fibroid- making right diagnosis
Adenomyosis or Fibroid- making right diagnosis
 
Physiotherapy Management of Rheumatoid Arthritis
Physiotherapy Management of Rheumatoid ArthritisPhysiotherapy Management of Rheumatoid Arthritis
Physiotherapy Management of Rheumatoid Arthritis
 
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdfSGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
SGK RỐI LOẠN KALI MÁU CỰC KỲ QUAN TRỌNG.pdf
 
The Importance of Mental Health: Why is Mental Health Important?
The Importance of Mental Health: Why is Mental Health Important?The Importance of Mental Health: Why is Mental Health Important?
The Importance of Mental Health: Why is Mental Health Important?
 
Mental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil ThirusanguMental health Team. Dr Senthil Thirusangu
Mental health Team. Dr Senthil Thirusangu
 
introduction to neurology (nervous system, areas, motor and sensory systems)
introduction to neurology (nervous system, areas, motor and sensory systems)introduction to neurology (nervous system, areas, motor and sensory systems)
introduction to neurology (nervous system, areas, motor and sensory systems)
 
Microbiology lecture presentation-1.pptx
Microbiology lecture presentation-1.pptxMicrobiology lecture presentation-1.pptx
Microbiology lecture presentation-1.pptx
 
CPR.nursingoutlook.pdf , Bsc nursing student
CPR.nursingoutlook.pdf , Bsc nursing studentCPR.nursingoutlook.pdf , Bsc nursing student
CPR.nursingoutlook.pdf , Bsc nursing student
 
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdfSGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
SGK RỐI LOẠN TOAN KIỀM ĐHYHN RẤT HAY VÀ ĐẶC SẮC.pdf
 
How to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturallyHow to cure cirrhosis and chronic hepatitis naturally
How to cure cirrhosis and chronic hepatitis naturally
 

Dsp of vaccine

  • 1. A SEMINAR ON Downstream processing of vaccine (e.g. Influenza & Tetanus) Presented By- NIYAMAT M. PANJESHA SNEHA SAWLE YUGENDRA PATIL Modern College Of Arts , Science & Commerce. Shivajinagar, Pune-05.
  • 2. What are Vaccines? • A vaccine is a biological preparation that improves immunity to a particular disease. • Name derived from the word vacca which means cow. • Can be- 1) Prophylactic. 2) Therapeutic. Edward Jenner observed that the farmers and milkmaids working with cows developed a mild form of small pox called cowpox or vaccinia. The cowpox infection could protect these people from infection of smallpox. It was in 1796 Jenner experimentally tested this observation. He inoculated an 8 yr old boy with exudates from a cowpox lesion and he repeated it twice with a gap of some weaks. Jenner noticed that smallpox did not develop in this boy. This was 1st discovery of principle of vaccination.
  • 3. Live attenuated Organism Killed Traditional Toxoid Sub-unit Types Recombinant Conjugate Vector DNA Experimental vaccine Edible vaccine
  • 4. Examples of Vaccines Sr. Vaccine Organism Disease No. 1 Live Attenuated Bacterial •Bacillus anthracis •Aanthrax Vaccine •Salmonella typhii •Typhoid 2 Inactivated Bacterial Vaccines Capsular Ag •Neissseria meningitides •Meningitis Cell wall Ag •Vibrio Cholera •Cholera Toxoid Ag •Clostridium tetani •Tetanus 3 Recombinant Vaccines Bacterial •Clostridium tetani •Tetanus Protozoal •Plasmodium vivax •Malaria Viral -H1N1 •Influenza 4 DNA vaccines •Malaria
  • 5. Steps In Vaccine Production
  • 6. processing SELECTING THE STRAIN FOR Upstream VACCINE PRODUCTION GROWING THE MICRO-ORGANISM ISOLATION & PURIFICATION OF MICROORGANISM Downstream processing INACTIVATION OF ORGANISM FORMULATION OF VACCINE QUALITY CONTROL AND LOT RELEASE
  • 7. Influenza Vaccine Two modes: Seasonal Epidemic and Pandemic Influenza Types of Vaccine Production: 1.Egg based(Traditional) 2.Cell line based Three forms of vaccine: A.Whole B.Split C.Subunit/Surface Antigen
  • 8. Live Virus Vaccine Production using MDCK cell line on Cytodex3 micro carrier Upstream Process: 0.5X106 cells/ml(at 100r.p.m. for 48hrs.) At 2-2.5X106 cells/ml infect with Viral inoculum and carry out for 24hr.
  • 9. DOWNSTREAM PROCESSING 1.Clarification: ULTA Prime GF capsule Filtration(0.6µm) 2.Diafiltration(0.4µm) in buffer[20mM Tris+0.5M NaCl, pH7.5] 3.DNA binding Anion Exchange Chromatography: To remove gDNA 4.Sterile Filtration(0.2µm): Stainless steel filter precoated with buffer.
  • 10. Tetanus is an acute and highly fatal disease caused by exotoxins produced by the bacterium Clostridium.tetani. Cl. tetani produces two exotoxins- tetanolysin and tetanospasmin. Tetanus toxoid is one of the most immunogenic antigens available for the protection against an infectious disease in the world. Tetanus Vaccine produced by anaerobic fermentation.
  • 11. Tetanus Vaccine Production Revival of the Preparation of seed Production strain production strain culture Production of tetanus toxin by Determination of Harvesting of Toxin Static Culture antigenic content Method Detoxification of the Partial purification Purification of Toxin of tetanus toxin crude tetanus toxoid
  • 12.    Production strain Inoculation Seed culture Fermentation Purification  Monosodium   Formaldehyde  Glycine  glutamate Alum soln soln Stabilizer Adjuvant Dilutant Inactivation Ultrafilteration  Thimersol     Preservative Inspection Labeling Packaging Filling 12
  • 14. REFERENCES Nielsen, P. A.1967. Large-Scale Production of Vaccine. Applied Microbiology. Ozutsumi K, Sugimoto N, and Matsuda M. 1985. Rapid, Simplified Method for Production and Purification of tetanus toxin. Applied and environmental microbiology.  Chandani Payal and Tejpal Kashyap, 2011. Production, Optimization of Detoxification and Ammonium Sulphate Precipitation of Ultrafiltered Tetanus Toxin. Recent Research in Science and Technology, 3(11): 49-54.  Nduka Okafor, Modern industrial microbiology and biotechnology. U.Satyanarayan, A Text book of Biotechnology. Michael J.Waites, Industrial Microbiology: An Introduction. GE Healthcare Life Sciences, Application note 28-9843-41 AA Vaccines Propagation and purification of influenza virus.
  • 15. QUESTIONS, CRITICISMS, AND SUGGESTIONS? THANK YOU snehasawle@rediffmail.com niyamatpanjesha@yahoo.com