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Blood 2008-sohn-1690-9
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2008 111: 1690-1699
Prepublished online November 1, 2007;
doi:10.1182/blood-2007-07-102335
Redistribution of accumulated cell iron: a modality of chelation with
therapeutic implications
Yang-Sung Sohn, William Breuer, Arnold Munnich and Z. Ioav Cabantchik
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BLOOD, 1 FEBRUARY 2008 VOLUME 111, NUMBER 3 REDISTRIBUTION OF IRON AS THERAPEUTIC STRATEGY 1691
Table 1. Iron binding parameters of agents used in this study tion onto 96-well plates, or onto microscopic slides attached to perforated
Iron ligands (abbreviations) pFe(III)* tissue culture 3-cm plates.
Probe loading into cells. For cytosolic loading of CALG, cells in
Calcein green (CALG) 20.3†
DMEM plus 10mM Na-HEPES (DMEM-HEPES) at 37°C were exposed
Deferrioxamine (DFO) 26.6
for 10 minutes to 0.25 M CALG-AM, washed and incubated in
Deferiprone (DFP) 19.3
DMEM-HEPES containing 0.5 mM probenecid (to minimize probe leak-
Diethylene-triamine-pentaacetic acid (DTPA) 28.2
age). For CALG-Fe(III) (1:1) complexes and for sulforhodamine loading
Nitrilotriacetic acid (NTA) 18.1
into endosomes, cells were exposed to 50 M to 100 M complex in
Transferrin (Tf) 22.3
DMEM-HEPES for 30 minutes at 37°C and washed extensively with the
Rhodamine B- (1, 10-phenanthrolin-5-yl) 7.7 (11.5 for Fe(II))‡
same probe-free medium and subsequently with HEPES-buffered saline
aminocarbonyl benzyl ester (RPA)
(HBS; 20 mM HEPES, 135 mM NaCl; pH 7.4).20,21 RPA was loaded into
*pFe(III) values are log M , where M is the concentration of the free metal ion mitochondria as described in earlier studies.21-23
when ligand 10 M and Fe(III) 1 M at pH 7.4. Data from Harris.17 Histone-CALG. For loading into the nucleus, CALG was coupled to
†Data from Thomas et al.18 core histones from calf thymus (H) 7.2 mg/mL, in 50 mM Na-MES,
‡Values shown are for 1,10-phenanthroline.
100 mM NaCl, pH 5.5, containing 1.25 mM CALG:Cobalt (Co protects
CALG metal-binding groups). Coupling was initiated by adding 12 mM
EDC, followed by incubation for 1 hour at room temperature and overnight
found to reduce iron accumulation in the dentate nuclei of at 5°C. After exhaustive dialysis, 11 mM DTPA was added (to remove
Friedreich ataxia patients,13 indicating its ability to cross the Co(II)) and histone-CALG (H-CALG) was further dialyzed against HBS.
blood-brain barrier in humans, which is consistent with previous CALG coupling to H was estimated at 1.1:1 by fluorescence ( excitation
findings in animals.14-16 480 nm, emission 520 nm). H-CALG-Fe was prepared by titrating CALG
In the present study we assess DFP’s capacity to act as an with FAS. Loading 10 M H-CALG or H-CALG-Fe into cells was done in
iron-relocating agent at the cellular level. To trace labile iron DMEM-HEPES containing 100 M chloroquine (for improved FMS
mobilized by chelators, we used model cardiac and macrophage targeting to cell nuclei),24 followed by HBS washes.
cells in culture and fluorescent metal sensors (FMS) targeted to Fluorescein-DFO-histone. The probe was prepared by coupling of
cellular and extracellular compartments. We show that DFP can N-(fluorescein-5-thiocarbamoyl)-desferrioxamine (Evrogen, Moscow, Rus-
sia) to H using fluorescein-DFO (FlDFO)–Fe (1:1) complex generated by
relocate iron accumulated in cell compartments within and across
adding 1.1 mM FeSO4 to 1 mM FlDFO in HBS. To 2.64 mL of FlDFO-Fe
the cell, and we demonstrate that iron withdrawn from sites of cell
complex was added 8.5 mg H, followed by 0.18 mL of 1 M MES (Na form),
accumulation can be safely transferred to extracellular transferrin pH 6.5, and 15 mg of EDC. After overnight agitation at 5°C, the mixture
for physiologic reuse. was dialyzed (3.5 kDa cut-off tubing) against 10 mM acetic acid;
1 mM EDTA; 150 mM NaCl, pH 4.5 (to remove bound iron); and finally
HBS. The fluorescein-DFO-histone (H-FlDFO) ratio was 1:1, based on
stoichiometric iron-quenching of H-FlDFO fluorescence ( excitation
Methods 494 nm, emission 520nm). H-FlDFO, like H-CALG, was loaded into cells
in DMEM-HEPES.
Materials
Rhodamine-labeled apotransferrin. Human apotransferrin (4 mg/mL
Calcein green (CALG) 3,3 -bis[N,N-bis(Carboxymethyl) aminomethyl]fluo- in 25 mM Na2CO3, 75 mM NaHCO3; pH 9.8), was incubated at 5°C
rescein and its acetomethoxy (AM) precursors CALG-AM, lissamine overnight with 1 mM lissamine rhodamine sulfonyl chloride and the labeled
rhodamine sulfonyl chloride, and sulforhodamine were from Molecular protein isolated by gel filtration on Sepharose G25 (Sigma-Aldrich)
Probes (Eugene, OR). Human serum holotransferrins and apotransferrins preequilibrated with 150 mM NaCl, 20 mM MES; pH 5.3.
(aTf) were from Kamada (Rehovot, Israel). Diethylene-triamine- Transfer of iron from DFP-Fe to apotransferrin. DFP-Fe complexes
pentaacetic acid (DTPA), nitrilotriacetate (NTA), EDC (1-ethyl-3-(3- were generated by incubating Fe-NTA (50:150 M) with either 150 M or
dimethylaminopropyl) carbodiimide), ferric ammonium citrate (FAC), 250 M DFP in HBS for 20 minutes, and completion of complex formation
ferrous ammonium sulfate (FAS), succinyl acetone, HEPES (N-2- was assessed by absorption at 455 nm. The complexes were mixed with
hydroxyethylpiperazine-N -2-ethanesulfonic acid), 4-morpholine ethanesul- 50 M aTf and 25 mM NaHCO3 and incubated for 1.5 hours in a 5% CO2
fonic acid (MES), core histone mixture from calf thymus (H), N,N - incubator. Because of the large spectral overlap of DFP-Fe and transferrin-
hexamethylene-bis-acetamide, and octyl-glucoside were from Sigma- Fe, we added DTPA (10 mM) to selectively scavenge iron from DFP-Fe.
Aldrich (St Louis, MO). Rhodamine isothiocyanate was from Fluka (Buchs, Transferrin that was free of low-molecular components was obtained by
Switzerland). DFP (1,2-dimethyl-3-hydroxypyridin-4-one) was from Apo- either filtering 0.5 mL through 30-kDa cut-off spin filters (Pall, East Hills,
Pharma (Toronto, ON). DFO was from Novartis (Basel, Switzerland). The NY) via centrifugation (2900g for 20 minutes), or by use of a 5-mL
red fluorescent mitochondrial metal–sensor rhodamine B-[(1, 10-phenanth- dry-spin–gel filtration-centrifugation at 1100g over precentrifuged Seph-
rolin-5-yl aminocarbonyl] benzyl ester (RPA), was a kind gift from U. adex G-50 medium columns (Sigma-Aldrich). The complex-free transferrin
Rauen and R. Sustmann, University of Duisburg-Essen, Essen, Germany. was diluted in HBS, pH 7.4. Tf-Fe was analyzed by absorbance at 465 nm,
A summary of the probes used and their properties is given in Table 1. and aTf by tryptophan fluorescence (280 nm excitation, 306 nm emission;
Felix spectrofluorimeter station version 2.5; Photon Technology Interna-
tional, Lawrenceville, NJ).
Methods
Iron-loading of cells was done by overnight incubation of cells with
Complexes of iron with NTA (Fe-NTA) were generated by mixing FAS and 100 M FAC in culture conditions followed by washing with HBS
NTA (1:3 molar ratio) in water and allowing iron to oxidize in ambient containing 100 M DFO, and with HBS alone. To achieve selective
conditions. The DFP-Fe(III) complexes were generated by mixing Fe-NTA accumulation of iron in mitochondria, cells were treated with 1 mM
with DFP in water; formation of fully substituted DFP-Fe complexes was succinyl acetone for 3 hours at 37°C.
confirmed by measuring absorbance at 455 nm.19 Cell hemoglobin synthesis. MEL cells cultured for 2 days in media
Cell-culture lines. Rat H9C2 cardiomyocytes, J774 mouse macro- supplemented with 5mM hexamethylene-bis-acetamide were washed and
phages, and erythroleukemia (MEL) were grown in 5% CO2 Dulbecco- lysed with octyl-glucoside (1.5%). After 2 minutes centrifugation at
modified Eagle medium (DMEM) supplemented with 10% fetal calf serum, 12 000g, 100 L of lysate supernatants was transferred to a 96-well
4.5 g/L D-glucose, glutamine, and antibiotics (Biological Industries, microplate for estimating hemoglobin (by measuring absorbance at
Kibbutz Bet Haemek, Israel). Cells were plated 1 day before experimenta- 410 nm). Alternatively, lysates were mixed with 100 L freshly prepared
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1692 SOHN et al BLOOD, 1 FEBRUARY 2008 VOLUME 111, NUMBER 3
Figure 1. Transfer of iron from extracellular DFP-iron
complexes to mitochondria. H9C2 cells loaded with the
mitochondrial iron-sensor RPA were incubated with or
without DFP-Fe (15:5 M) and epifluorescence micro-
scopic images were recorded every 5 minutes under
settings for rhodamine. Representative fields of initial cell
fluorescence at time 0 (A,C) and after incubation for 1 hour
in the presence (B) or absence (D) of 5 M DFP-Fe
complex. Magnification was 600; oil objective was a
(Plan Apo) 60 /1.40 NA. (E) Mean fluorescence values in
relative units (r.u.) plus or minus SD of 5 cells per field
calculated for each time-point image and normalized to the
initial fluorescence, representing cells incubated without
(None) and with 5 M Fe-complex (DFP-Fe or NTA-Fe);
the lines denoted as DFP-Fe and NTA-Fe were cor-
rected for spontaneous decay given by the control (None).
Arrow indicates time the Fe complex was added. (F) Effect
of various iron chelates on the fluorescence (f normalized
to initial value, f0, in r.u.) of 0.5 M RPA in solution (HBS
buffer). The iron chelates all contained 5 M Fe complexed
to NTA (1:3 ratio) in the absence (f) and presence ( ) of
100 M ascorbate (Fe:NTA, Fe:NTA ASC) or to DFP,
with 100 M ascorbate, at DFP:Fe ratios of 3:1 (F), 5:1 (E),
and 7:1 (‚). The values of fluorescence intensity are
means of triplicate samples run in parallel, plus or
minus SEM.
tetramethylbenzidine (0.5 mg/mL in 10% acetic acid) and finally 8 L of endogenous labile iron and/or to probe photobleaching. On the
30% H2O2. Absorption (604 nm) was read after 90 seconds. other hand, addition of preformed DFP-Fe(III) complexes (DFP-
Data acquisition and analysis. Epifluorescence imaging (Nikon TE Fe, ratio 3:1) evoked a time-dependent (t ⁄ 15 minutes) quenching
12
2000 microscope; Melville, NY; and Orca-Era CCD camera; Hamamatsu,
of mitochondrial RPA fluorescence (Figure 1E). Consistent with
Bridgewater, NJ) coupled to a Volocity 4 system (Improvision, Coventry,
United Kingdom) was used for image data acquisition and analysis.20 For RPA’s selectivity for Fe(II), no quenching of RPA by Fe-NTA
high-throughput fluorescence monitoring we used fluorescence plate read- occurred in solution unless ascorbate was added. Transfer of iron
ing (Tecan-Safire; Neotec, Mannedorf, Austria) essentially as described.20
¨ from DFP-Fe complexes to RPA in the presence of ascorbate was
detected over a wide range of DFP to Fe ratios (1:1 to 7:1).
Transfer of iron from extracellular DFP-Fe complexes to
Results nuclei. With the view of targeting a fluorescent iron sensor into
the cell nucleus, we constructed a conjugate of H with the
To follow DFP-mediated translocation of iron from the medium to high-affinity iron chelator and iron sensor H-FlDFO.11 To minimize
cells, within cells, and from cells to the medium, we used uptake via the endocytic pathway, incubation was carried out at
fluorescent acceptors and donors of iron that undergo quenching or room temperature and in the presence of chloroquine, as described
dequenching upon metal binding or removal. An additional feature in “Histone-CALG.” H-FlDFO within nuclei was rapidly
of these probes was their localization in specific cell compartments (t ⁄
12 5 minutes) quenched by DFP-Fe (3:1) added to the extracel-
or in the extracellular medium. lular medium (Figure 2E), consistent with the high cell permeabil-
ity of DFP-Fe complexes shown in Figure 1. In solution, H-FlDFO
Transfer of iron from extracellular DFP-iron complexes to
is efficiently and rapidly (t ⁄12 1 minute) quenched by DFP-Fe
mitochondria
complexes even at a 9:1 DFP:Fe ratio (Figure 2F), as expected from
Ingress of ionic iron from the extracellular medium to the DFP’s capacity to shuttle iron to DFO both in vivo and in vitro.11
mitochondria of H9C2 cardiomyocytes was followed with the DFP-mediated redistribution of iron between cellular compart-
high-affinity Fe(II)–acceptor probe, RPA22,23 (Figure 1). The hydro- ments: from nuclei to mitochondria. DFP’s facilitation of iron
phobic cationic rhodamine B is responsible for RPA’s potentiomet- movement between discrete cell compartments was followed in
ric partitioning in the mitochondria, and phenanthroline is respon- H9C2 cells sequentially labeled in cell nuclei with the iron-donor
sible for detection of Fe(II).22 Exposure of cells to Fe(III)-NTA probe H-CALG-Fe(III) and in mitochondria with the Fe(II)-
evoked no significant changes in RPA fluorescence relative to the acceptor probe RPA. H-CALG-Fe penetrates the plasma membrane
spontaneous decrease (t ⁄ 60 minutes), probably due to binding of
12 and accumulates initially in the cytosol and subsequently in the
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BLOOD, 1 FEBRUARY 2008 VOLUME 111, NUMBER 3 REDISTRIBUTION OF IRON AS THERAPEUTIC STRATEGY 1693
Figure 2. Transfer of iron from extracellular DFP-iron complexes to nuclei.
H9C2 cells loaded with the nuclear iron sensor H-FlDFO were incubated with or Figure 3. DFP-mediated relocation of iron between cellular compartments:
without 5 M DFP-Fe complex (DFP:Fe ratio 3:1) and epifluorescent microscopic from nuclei to mitochondria. H9C2 cells were loaded with the nuclear iron sensor
images were recorded every 5 minutes under settings for fluorescein. Representative H-CALG that had been precomplexed to iron (H-CALG-Fe), followed by loading with
fields of initial cell fluorescence at time 0 (A,C) and after incubation for 1 hour in the the mitochondrial iron sensor RPA. The double-labeled cells were then exposed to
absence (B) or presence (D) of 5 M DFP-Fe complex. (E) Mean fluorescence values 50 M DFP and epifluorescence images were recorded every 5 minutes. Representa-
in r.u. plus or minus SD of 5 cells per field, calculated for each time-point image and tive fields of cell fluorescence observed under settings for fluorescein (A,B) and
normalized to the initial fluorescence (f/f0), representing cells incubated without rhodamine (C,D). Images are shown at time 0 (A,C) and after incubation for 1 hour in
(None) and with 5 M DFP-Fe complex (DFP-Fe). Arrow indicates time the Fe the presence of 50 M DFP (B,D). (E) Mean fluorescence values plus or minus SD in
complex was added. (F) Effect of DFP-Fe chelates (DFP:Fe at ratios of 1:1, 3:1, 5:1, r.u. of 5 cells per field, calculated for each time-point image and normalized to the
9:1) on the fluorescence (normalized to initial value, f0, in r.u.) of 0.5 M H-FlDFO in initial fluorescence (f/f0), representing cells incubated with 50 M DFP (circles) and
solution (HBS buffer). The values of fluorescence intensity are means of triplicate with no addition (squares). Fluorescence of H-CALG is indicated by filled symbols
samples run in parallel, plus or minus SEM. (F and f), and fluorescence of RPA is indicated by open symbols (E and ). The
scheme illustrates entry of DFP into the cytosol (C), nuclei (N) containing H-CALG-Fe
(iron donor) and mitochondria (M) containing RPA (iron acceptor), and transfer of iron
from nuclei to mitochondria. E refers to endosomes.
nucleus, particularly in nucleolus-like sites.21 H-CALG-Fe was
used subsaturated with iron so that its accumulation in the nuclei
could be followed via changes in the residual fluorescence of the
CALG-Fe into endosome/pinosomes and its accessibility to DFP, as
unquenched fraction of the probe (revealed by chelation; Figure
evidenced by dequenching, in a previous study.20 Moreover, CALG-Fe
3A). Loading of RPA into mitochondria was apparent from the
coendocytosed with the classical fluid phase markers sulforhodamine
characteristic perinuclear staining pattern (Figure 3C), from colo-
and rhodaminated dextran, both of which showed less than 65% cell
calization with other potentiometric probes and sensitivity to
colocalization as obtained with the Volocity program (not shown).
protonophores.22,23 Addition of 50 M DFP produced an increase in
the fluorescence of H-CALG-Fe in the nuclei and a concomitant H-FlDFO was detectable in the nuclear area, while CALG-Fe was
decrease in the fluorescence of RPA in the mitochondria, both of concentrated in punctuate, perinuclear spots, attributable to endosome-
which occurred within 5 to 8 minutes and were complete within trapped CALG-Fe (Figure 4). However, after incubation with 50 M
20 minutes (Figure 3E). The changes are attributable to dequench- DFP, the fluorescence in the endosomes increased significantly, while
ing of H-CALG-Fe due to removal of iron by DFP and to that in the nuclei decreased relative to the respective untreated controls.
quenching of RPA due to transfer of iron from DFP-Fe. Only minor The kinetics of the changes in the 2 compartments followed similar
changes in fluorescence were observed in untreated control cells. It patterns, although in opposite directions. To ascertain whether the iron
is likely that nuclear H-CALG-Fe was not the only source of iron acquired by DFP was exclusively intracellular, the cells were washed
delivered to RPA by DFP, because the addition of DFP to with 0.1 mM DFO to remove all extracellular iron before the addition of
RPA-loaded cells also led to quenching of mitochondrial probe DFP. Furthermore, as the addition of DFP to cells loaded with H-FlDFO
(data not shown), although to a much lesser extent than in the alone failed to produce a decrease in its fluorescence (data not shown),
presence of H-CALG-Fe. the quenching of H-FlDFO in cells coloaded with CALG-Fe is most
DFP-mediated relocation of iron between cellular compart- likely due to DFP-mediated mobilization of iron from endosomes.
ments: from endosomes to nuclei. H9C2 cells were labeled However, the possibility that in the course of the experiment some
sequentially in the nuclei with the iron-acceptor probe H-FlDFO and in iron might have spontaneously leaked from endosomes to cytosol
the endosomes with the iron-donor probe CALG-Fe (Figure 4). We and rendered it transferable to mitochondria by added DFP cannot
showed the bulk-phase pinocytic uptake of the iron-quenched probe be ignored.
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1694 SOHN et al BLOOD, 1 FEBRUARY 2008 VOLUME 111, NUMBER 3
Figure 4. DFP-mediated relocation of iron between
cellular compartments: from endosomes to nuclei.
H9C2 cells were loaded with the nuclear iron-sensor
H-FlDFO and subsequently exposed to preformed
complexes of CALG and iron (CALG-Fe, 1:1 ratio) that
were taken up by adsorptive pinocytosis into endo-
somes. The cells containing both labels were then
exposed to 50 M DFP and epifluorescent images
were recorded every 5 minutes under fluorescein set-
tings. Representative fields of cell fluorescence are
shown at time 0 (A) and after incubation for 20 minutes
(B) in the presence of 50 M DFP. (C) The nuclei
(N) and endosomes (E) are indicated by arrows.
(D) Mean fluorescence values in r.u. within selected
subcellular areas corresponding to endosomes
(Di) and nuclei (Dii), using 5 cells per field, calculated
for each time-point image and normalized to the initial
fluorescence (f/f0).
Transfer of iron from DFP-Fe complexes to transferrin. We The rationale was that intracellular iron mobilized to the extracellu-
followed iron complexation by transferrin using DFP-Fe com- lar medium by DFP would be transferred to the fluorescent Tf,
plexes (3:1 and 5:1, 50 M in Fe) and NTA-Fe complexes (3:1, which, upon binding iron, would then bind to cell-surface trans-
50 M in Fe) as iron sources and apotransferrin (50 M) as ferrin, and after receptor-mediated endocytosis, would become
acceptor. To remove from the reaction mixture any traces of iron concentrated in endosomes. To increase the level of mitochondrial
associated with low molecular weight complexes or free ligands, labile iron, the cells were pretreated with the heme-synthesis
we added DTPA after 1 hour of reaction and followed it by size inhibitor succinylacetone, which causes selective accumulation of
filtration. Filtration and differential chelation allowed the assess- unused, chelatable iron in the mitochondria.22 After 1 hour of
ment of DFP-Fe transfer of iron to aTf both by light absorption of incubation with DFP, the level of cell-bound fluorescent aTf rose
Tf-Fe complexes at 465 nm (Figure 5) and by tryptophan fluores- by 2.3-fold over controls without DFP (Figure 6A,B). The binding
cence of aTf that is quenched after specific binding of iron25 was specific and iron dependent, as it was fully blocked by excess
(Figure 5). While the 2 analytical methods monitored binding of holotransferrin (Figure 6C,D) and enhanced by addition of saturat-
iron to transferrin, the optical changes are mirror images of each ing concentrations of Fe-NTA (Figure 6E,F), both in the presence
other. Transfer of iron from DFP-Fe to transferrin ensued at both and in the absence of DFP. In control cells not treated with
3:1 and 5:1 stoichiometries of DFP:Fe and was of a stable nature: it succinylacetone, DFP failed to enhance fluorescent-Tf binding
persisted after addition of chelators that differentially dissociate (data not shown), indicating that under normal conditions where
DFP-Fe complexes (but not Fe-transferrin). On the other hand, iron incorporation into heme is not blocked, the concentration of
DFP alone and NTA alone evoked only minor changes that were DFP-mobilized iron is insufficient to be detectable in this system.
eliminated by addition of DTPA before the reaction with aTf, DFP-mediated iron delivery for hemoglobin synthesis. The
indicating that the changes were associated with traces of iron potential of DFP-Fe complexes as donors of iron for physiologic
present in the medium. Essentially similar results were obtained by use was assessed using a model system of cellular iron incorpora-
assessing the transfer of iron to apotransferrin using fluorescein- tion, synthesis of hemoglobin (Hb) by differentiating preerythroid
labeled aTf (not shown). However, with lissamine rhodamine- cells. The well-documented induction of Hb synthesis in MEL cells
labeled aTf (R-aTf), the acquisition of iron evoked insignificant by hexamethylene bisacetamide (HMBA) is highly dependent on
changes in fluorescence. the supply of iron.26 It is repressed in serum-free medium lacking
transferrin, but it is significantly enhanced by the addition of
DFP-mediated mobilization of iron from H9C2 cells to
Fe-NTA irrespective of the presence of Tf (Figure 7). DFP-Fe (3:1)
extracellular transferrin: receptor-mediated uptake of
supported Hb synthesis to a degree similar to that seen with
holotransferrin as an index of iron shuttling
Fe-NTA, both in the absence and presence of added Tf. Fully
Mobilization of cellular iron by DFP and its transfer to extracellular saturated holotransferrin (15 M) was equally effective as Fe-NTA
transferrin are critical features of DFP’s potential function as an and DFP-Fe (data not shown), indicating that various forms of iron
iron shuttle. This capability was assessed using H9C2 cells can equally support Hb synthesis in this experimental system. On
incubated with lissamine R-aTf in the presence of DFP (Figure 6). the other hand, DFP without added iron depressed Hb synthesis
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BLOOD, 1 FEBRUARY 2008 VOLUME 111, NUMBER 3 REDISTRIBUTION OF IRON AS THERAPEUTIC STRATEGY 1695
Figure 5. Transfer of iron from DFP-Fe complexes
to transferrin. DFP-Fe complexes (1:3 and 1:5 ratios;
50 M Fe(III)) were mixed with apotransferrin aTf
(50 M) and incubated for 1.5 hours in a humidified 5%
CO2 incubator at 37°C. At the conclusion of the reac-
tion, 10 mM DTPA was added and the low–molecular
weight material was removed by filtration as described
in “Histone-CALG.” The tryptophan (trp) fluorescence
at 280 nm excitation/306 nm emission (top graph) was
obtained from the emission spectra (inset). The high–
molecular weight fractions were diluted in HBS, pH 7.4,
and the absorbance was read at 465nm (bottom graph).
Data are given as means plus or minus SD of
3 independent experiments. The labels below the bars
indicate the complexes tested and their concentrations.
The values above the bars represent the percentage
change in absorbance or fluorescence.
below control levels, indicating that the chelator can have an (data not shown), an effect similar to DFO-induced iron depriva-
iron-withdrawing effect under conditions of limited iron supply. tion in other hematopoietic cells.27 To demonstrate that transfer of
In the serum-free medium used in these experiments, DFP iron from DFP-Fe to aTf gave rise to functionally active holotrans-
( 100 M) without added iron, as well as DFO (100 M), ferrin, DFP-Fe was allowed to interact with aTf for 1 hour, and then
prevented differentiation and ultimately caused massive cell death the mixture was exhaustively dialyzed (cut-off 12 kDa) against
Figure 6. DFP-mediated mobilization of iron from
H9C2 cells to extracellular apotransferrin: receptor-
mediated uptake of holotransferrin as an index of iron
transfer. H9C2 cells were pretreated with succinylac-
etone as described in “Methods” to increase mitochon-
drial labile iron levels. They were then incubated at
37°C in DMEM-HEPES medium containing 20 M
lissamine R-aTf with various additions, and epifluores-
cence microscopy images were obtained under rhodamine
settings after 60 minutes of incubation. Representative
images of cells with no addition (A), 30 M DFP (B),
100 M unlabeled holotransferrin (C), 100 M unlabeled
holotransferrin with 30 M DFP (D), 20 M Fe-NTA, 1:3
ratio (E), and 20 M Fe-NTA with 30 M DFP (F). (G) The
illustration represents entry of DFP into cells, mobilization
of iron from the cytosol (C), nuclei (N) and mitochondria
(M), followed by exit of DFP-Fe complexes from the cells
(step 1). This is followed by transfer of iron from DFP-Fe to
R*-aTf to form R*-Tf-Fe (step 2), which then binds to
transferrin receptors on the cell surface (step 3), concen-
trates in the endosomes, and is detected by fluorescence
microscopy as punctuate fluorescence typical of mi-
crovesicles. (H) Mean cell-associated fluorescence values
in r.u. of 5 cells per field ( SD, from 3 separate experi-
ments), calculated from snapshots such as shown in
panels A through F, obtained after 1 hour of incubation.
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1696 SOHN et al BLOOD, 1 FEBRUARY 2008 VOLUME 111, NUMBER 3
Figure 7. Stimulation of Hb synthesis in murine erythroleukemia cells. (A) DFP-mediated iron delivery to murine erythroleukemia (MEL) cells synthesizing hemoglobin is
schematically depicted. (B) MEL cells suspended in serum-free DMEM containing 1.25 mg/mL bovine serum albumin and 5 mM HMBA were supplemented with various iron
complexes (step 1 in panel A) and cultured for 48 hours. Hemoglobin synthesis (step 2 in panel A) was assessed in terms of hemoglobin content in cell lysates as described in
“Cell hemoglobin synthesis” and is expressed relative to the hemoglobin content in control cells with no additions (set as 100%). The additions included 10 M Fe complexed to
30 M NTA without (Fe-NTA) and with 15 M human apotransferrin (Fe:NTA aTf); 10 M Fe complexed to 30 M DFP without (DFP-Fe) and with 15 M human
apotransferrin (DFP-Fe aTf); 30 M DFP alone (DFP); 15 M human apotransferrin alone (aTf). Generation of holotransferrin from DFP and apotransferrin (DFP-Fe aTf
Dial.): 10 M Fe:30 M DFP complex was preincubated with 15 M human for 1 hour and dialyzed. Results shown are averages of 3 separate experiments plus or minus SD;
the average Abs604 of control cells was 0.39. (C) DFP-mediated mobilization of iron from J774 (Fe donor; step 1a in panel A) cells to the extracellular medium (step 1b in panel
A), followed by entry of DFP-Fe complexes into MEL (Fe acceptor) cells (step 1c in panel A) and intracellular donation of iron for hemoglobin (Hb) synthesis (step 2 in panel A).
MEL cells were cultured for 48 hours in DMEM containing 1.25 mg/mL bovine serum albumin and 5 mM HMBA without (Con) or with 10 M Fe-NTA (NTA-Fe), or with various
supernatants of J774 cell lysates that were supplemented with 5 mM HMBA, and lysates were assayed for hemoglobin content. To obtain J774 mouse macrophage
supernatants, J774 cells were cultured overnight without (untreated J774) or with 100 M FAC (Fe-loaded J774), washed with 100 M DFO to remove all extracellular iron, and
incubated for 2 hours at 37°C in serum-free DMEM containing 1.25 mg/mL bovine serum albumin, without ( DFP) or with 30 M DFP ( DFP). The cell supernatants were
collected and centrifuged to remove detached cells, and HMBA (5 mM) was added to MEL cells. Shown are values of hemoglobin (Hb; from a representative experiment)
obtained in MEL cells exposed for 48 hours to the various conditions.
buffer containing 80 mg/L aTf to remove DFP-Fe without gaining support for the proposed use of DFP as an iron shuttle even without
contaminant iron from the solution. When added to differentiating the mediation of transferrin, such as might occur in the central
MEL cells, this preparation, generated from DFP-Fe and aTf, fully nervous system.
supported Hb synthesis (Figure 7B).
Finally, we assessed DFP’s ability to shuttle iron from cell to
cell (Figure 7C). In vivo, direct intercellular transfer of iron via Discussion
DFP is not likely, due to the presence of more than 50 M due to
the presence of more than 25 M transferrin in plasma.28 However, The recognition that intracellular iron accumulation is one of the
intercellular transfer of iron via DFP could occur in the brain where underlying causes of some clinical syndromes has led to the
the iron-binding capacity of transferrin in cerebrospinal fluid (CSF) suggestion that it might be amenable to treatment with iron
is estimated to be in the submicromolar range.29,30 In the experi- chelators.3,33,34 These syndromes include Parkinson disease, neuro-
ment illustrated in Figure 7A, J774 mouse macrophages were degeneration with NBIA, and Friedreich ataxia, where iron depos-
chosen as the iron-donating cells because of their high capacity for its in specific areas of the brain have been identified by histologic or
iron accumulation.31 DFP at a concentration of 30 M, achieved in imaging techniques34 and are thought to be a central factor in the
vivo with moderate oral doses,1,32 mobilized sufficient iron from development of the diseases.35 Similarly, in anemia of chronic
iron-loaded J774 cells, but not from untreated ones, to significantly disease, iron is retained within erythrocyte-phagocytosing macro-
enhance Hb synthesis in MEL cells (Figure 7C). Spontaneous phages, presumably to prevent access by invading pathogens to
efflux of iron from iron-loaded J774 cells took place, as evidenced iron from the circulation.7-9,31 While regional iron mobilization
by the increased MEL cell Hb levels even in the absence of might be essential for stabilizing or reversing the toxic effects of
DFP (although increased, these levels were significantly lower in labile iron, in some pathologies it might be equally important to
the absence of DFP than in its presence). This result provides render the mobilized iron available for metabolic reuse. This is
9. From bloodjournal.hematologylibrary.org by guest on September 28, 2011. For personal use only.
BLOOD, 1 FEBRUARY 2008 VOLUME 111, NUMBER 3 REDISTRIBUTION OF IRON AS THERAPEUTIC STRATEGY 1697
especially important in those conditions in which regional iron hemoglobin synthesis when presented together with DFP or after
accumulation is accompanied by regional or systemic deprivation. DFP’s removal (Figure 7).
All the chelators in clinical use have been designed for massive The direct bioavailability of DFP-bound iron was investigated
removal and excretion of iron from organs of iron-overloaded using hemoglobin synthesis by cells in culture as a model. DFP-Fe
patients.2,10,33 Here we assessed the potential application of an iron supported hemoglobin synthesis even in the absence of transferrin
chelator in clinical use (DFP) as a shuttling agent capable of (Figure 7), consistent with a similar activity of 1:1 complexes of
redistributing iron within and among cells, while satisfying certain iron with salicylaldehyde isonicotinoyl hydrazone.43 More impor-
basic requirements. tant, DFP enhanced the transfer of iron from iron-loaded macro-
Permeability across cell membranes in the free and iron- phages to preerythroid cells for hemoglobin synthesis, without the
bound form. The high membrane permeability of the iron-free intermediary presence of transferrin (Figure 7). Such direct dona-
form of DFP is well documented, as shown by its capacity to tion to intracellular iron-requiring or iron-metabolizing enzymes
rapidly access and deplete intracellular labile iron pools.36,37 On the may not be a necessary feature if iron is efficiently transferred from
other hand, the ability of DFP-Fe complexes to traverse intracellu- the shuttle to circulating transferrin. However, it may be desirable
lar and plasma membranes of cells has not been studied in or even essential in the brain, where the iron-binding capacity of
detail.20,21,36,37 One indication is the capacity of orally administered transferrin in the CSF is negligible and has been estimated in the
DFP to increase serum transferrin saturation12 and labile iron11 submicromolar range.29,30
within an hour after intake in thalassemia patients, which is Low toxic potential of iron-shuttling agent complexes.
attributable to the rapid exit of DFP-Fe complexes from cells into A major concern in the use of chelators is the formation of iron
the circulation. At the intracellular level, it is shown in this work complexes that undergo redox cycling and thereby catalyze ROS
that DFP can facilitate the removal of labile iron from nuclei generation. This tendency is present in chelators with relatively low
(Figure 3), from endosomes (Figure 4), and from mitochondria redox potentials, such as those based on amine- and carboxyl-
(Figure 6), and can transfer iron to acceptors in the mitochondria liganding groups but not with the bidentate 3:1 complexing
(Figure 3), nuclei (Figure 4), and extracellular medium (Figure 6). hydroxypyridone DFP.20,21,44,45 However, speciation studies10 and
The transfer might involve dissociation of the DFP-Fe complexes recent examination of ROS production42 indicated that complete
abolition of labile iron by DFP is attained only at DFP:Fe(III) ratios
and/or formation of ternary complexes with other ligands.
close to 5:1. Whether DFP levels reached therapeutically are
Ability to compete effectively for intracellular labile iron with
consistently high enough to avoid formation of substoichiometric
other intermediate affinity species. Labile iron that is likely to be
redox-active complexes is difficult to predict. This is particularly
accessible to DFP is assumed to be bound to several possible
important in susceptible, DNA-containing organelles such as
ligands (eg, citrate, ATP) and to be in dynamic equilibrium between
nuclei and mitochondria. The same applies to intracellular signal-
Fe(II) and Fe(III) as dictated by the redox status of the intracellular
ing pathways activated by oxidative stress that could be generated
environment.38,39 DFP competes effectively for labile cell iron both
by DFP-mediated redistribution of cell iron.
in vitro and in vivo,32 and in individuals with normal iron balance it
Permeability across the blood-brain barrier (BBB) for thera-
raises serum iron levels, indicating mobilization of tissue iron.12
peutic applications to neurodegenerative diseases. The capacity
This was also observed with isolated cells (Figures 3,4), where
of an iron-shuttling agent to redistribute iron in the central nervous
DFP readily chelated iron bound to the EDTA analog CALG in
system (CNS) may be an essential requirement for iron redistribu-
endosomes and nuclei and was indicated in other systems.40 An
tion in Parkinson disease, Friedreich ataxia, or NBIA. A high rate of
undesirable property of a chelating molecule would be interference BBB penetration is expected for free DFP, based on its low
with normal cellular iron metabolism by overchelation. In this molecular weight (below 300) and lipophilicity. This was con-
respect, DFP ought to be used at restricted doses, as at high levels it firmed by direct measurement of DFP accumulation in the perfused
appears to inhibit iron-requiring tyrosine and tryptophan rat brain.15,16 In rats given DFP doses several times higher than
hydroxylases.10,14 analogous doses given to thalassemia patients, brain tyrosine and
Ability to donate iron to physiologic acceptors. The rationale tryptophan hydroxylase activities were significantly inhibited, as
behind DFP-mediated redistribution of iron leans on DFP’s ability measured by the accumulation of 3,4-dihydroxyphenylalanine
to transfer the metal to extracellular transferrin, thereby minimizing (DOPA) and 5-hydroxytryptophan, respectively, presumably via
undesired iron loss by excretion via the urinary or biliary pathways. coordination to iron bound by these enzymes.14 Yet, despite
We surmised that under physiologic conditions iron transfer to aTf DFP’s brain accessibility, major effects on CNS function have not
is likely to occur (1) spontaneously, as the pFe value of Fe(III) for been reported.32,44
transferrin is 3 orders of magnitude higher than for DFP41 (Table 1), Whether DFP can alter the iron balance in the brain by shuttling
and (2) directly as Fe(III), because the thermodynamically favored iron across the BBB remains an open question. The absence of iron
DFP-Fe complexes are (DFP)2-Fe(III) and (DFP)3-Fe(III).10,25,42 In loading of the CNS in iron-overload conditions indicates that
vitro studies based on an indirect analytical method11 corroborated neither transferrin-bound nor non–transferrin-bound iron is able to
earlier observations that a single oral dose of 3 g DFP raised within freely cross the BBB.35 Because DFP treatment has not been
6 hours the transferrin saturation (determined by urea-gel electro- reported to be associated with increased iron loading of the CNS in
phoresis) of a healthy individual from a normal level of 20% to thalassemia patients, it is conceivable that DFP-Fe complexes do
80%.12 In this work we provided direct demonstration of iron not readily permeate the BBB. The permeabilities of free and
transfer from DFP-Fe to aTf at physiologic concentrations (Figure iron-bound DFP may differ due to differences in their molecular
5). Using spectrophotometric and fluorimetric methods, we showed weights (473 for 3DFP:1Fe compared with 139 for DFP). In a
that efficient transfer occurs at various DFP:Fe ratios and within recent study, 6 months of treatment with DFP was shown to result
1 to 2 hours in culture conditions. The resulting holotransferrin in a decrease in the iron-associated magnetic resonance imaging
formed from DFP-Fe was biologically active, as it was recognized (MRI) transverse relaxation rate (R2*) signals in dentate nuclei of
by the transferrin receptor (Figure 6) and provided iron to cells for Friedreich ataxia patients.13 Whether this decrease was caused by
10. From bloodjournal.hematologylibrary.org by guest on September 28, 2011. For personal use only.
1698 SOHN et al BLOOD, 1 FEBRUARY 2008 VOLUME 111, NUMBER 3
egress of DFP-Fe complexes from the brain or by redistribution of iron loss.48,49 Optimization of such strategies with novel chelators
iron from an area of accumulation to areas of lower concentration for treating the various conditions of regional iron accumulation
within the brain is unclear. demand further laboratory and clinical work.
We show that redistribution of iron in multiple directions and to
multiple recipients is experimentally feasible, particularly from
areas of accumulation to areas of iron need, via donation to Acknowledgments
transferrin or by direct metal donation. A modality of iron chelation
based on iron redistribution has several therapeutic implications. In This work was supported by the Association Française contre les
the CNS, it could potentially be used to relocate local iron deposits Myopathies, the Israel Science Foundation (ISF), the EEC Frame-
work 6 (LSHM-CT-2006-037296 Euroiron1) and French-Israeli
that may be an important factor in the etiology of several
Organization for Research in Neuroscience (AFIRN). Z.I.C. is the
neurodegenerative diseases. In ACD, it could be used to release
Sergio and Adelina Della Pergolla Professor of Life Sciences.
iron trapped within macrophages. Current experimental approaches
to alleviation of ACD tend to be directed at depressing the levels of
hepcidin, the iron-regulator protein responsible for macrophage
Authorship
iron retention. However, recent evidence for hepcidin-independent
down-regulation of the iron exporter ferroportin by the inflamma- Contribution: Y.-S.S. and W.B. performed experimentation and
tory mediators46 could obviate such an approach. As shown in this analysis; A.M. and Z.I.C. designed and supervised the research. All
work, an alternate or complementary approach could be based on authors contributed to the writing of the paper.
iron-shuttling agents that relocate misdistributed iron and safely Conflict-of-interest disclosure: The authors declare no compet-
bypass impaired internal routes of iron trafficking. Early pilot ing financial interests.
studies with iron chelators in rheumatoid arthritis patients with Correspondence: Z. I. Cabantchik, Alexander Silberman Insti-
ACD47 provided clinical evidence that agents like DFP might be tute of Life Sciences, Hebrew University of Jerusalem, Safra
adopted as part of a short-term strategy of regional metal detoxifi- Campus at Givat Ram, Jerusalem, Israel 91904; e-mail:
cation and systemic relocation that generates no significant urinary ioav@cc.huji.ac.il.
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