coral mucus is the method of protection used by coral reef in sea water since it's a species specific a little studies hold on it and there's a lot of research to go on it.
2. Introduction
• Known functions of mucus
release are:
• Protection against fouling.
• Desiccation during air exposure at
extreme low tide.
• Sedimentation.
• Trap particulate matter from the
water.
• Light-collecting coral surfaces.
3. Introduction (cont.)
• Mucus is:
• coral-derived organic matter.
• complex mixture of secreted materials.
• species specific composition.
(The bacterial composition on the coral mucus depends on
the mucus chemical composition which relate to the coral
condition).
4. Aim of study
• To investigate the difference in the
bacterial composition of three
different coral species at three
different depths.
• Sources of natural products with
interesting biological activities.
• Bio-indicator method.
5. Materials and methods
• Study site:
• Southern Egyptian
Red sea coast.
• Sahl Hasheesh Bay is
home to a number of
islands and coral
reefs with excellent
diving and
snorkeling.
6. Materials and methods (cont.)
• Sampling:
• Diving to 5, 10 and 15 m.
• 3 species (Xenia, Stylophora and
Pocillopora).
• 50 ml falcon tubes.
• Samples collected with its
surrounding slime.
• Samples kept at ice box for lab.
examination.
12. Materials and methods (cont.)
• Purification of Bacterial
Isolates:
• Bacterial colonies with
different morphological
characteristics (representative
colonies) were removed.
• Streaked to a new plate of the
same media.
• Incubated at 28ºC for 24 h.
13. Results
• Three thousand two hundred sixty
six (CFU/ml) were counted at three
different media.
• Hundred fifty two representative
colonies were recognized.
• Comparison of detected bacteria was
carried out according to:
• Action at different media
• Depth
• Species
14. Results (cont.)
Action of media
Points of comparison SSW agar TCBs agar MAC agar
Bacterial action At all samples Hard coral only At all samples
Highest count Stylophora Pocillopora Pocillopora
Highest diversity Stylophora Stylophora Pocillopora
16. Results (cont.)
Action of species
Points of comparison Pocillopora sp. Xenia sp. Stylophora sp.
Bacterial action at the
three cultured media
Showing activity at all No action at TCBs at all
depths
Showing activity at all
Bacterial count
(CFU/ml)
1501 208 1557
Bacterial diversity 42 38 53
the composition of coral mucus has been recorded to vary temporally between coral species with water depth and irradiance level.
The adhesive mucus has the ability to trap particulate matter from the water and the light-collecting coral surfaces.
Mucus and trapped particles are transported over the coral surface by ciliary currents and are released to the water9 at a magnitude such that mucus can dominate suspended matter around reefs10, 11. Our measured
Coral mucus contains higher concentrations of organic matter, nutrients, and microbes such as viruses, bacteria, archaea, and protozoa.
Ice box refrigerator concept
Don’t forget el foil
TCBs don’t aautoclave
Ssw general media , tcbs media for vibrio ident
Mac for medical purposes specific media for differentiation of gram bacterial act
color, margin, size, elevation, and surface texture and shape
Morphological characterization and identification color. Texture and size , transperancy
Idea of representative colony
Make sure of contamination
Example vibrio of Xenia and some samples
Discussion of each action associated with medias
Liah el mediat action we feh not
The composition of coral mucus has been recorded to vary temporally between coral species with water depth and irradiance level.
Biological events, such as algal competition, reproduction, and diseases, as well as changes in environmental variables, including temperature, pH, nutrients, and dissolved organic carbon, generate shifts in the composition, richness, and abundance of coral-associated bacteria.
Bacterial comp as bioindicator for long or short term impact