1. In silico analysis of BAB71591 :a protein of unknown function deregulated during Hepatitis C in Homo sapiens PRESENTED BY: AFSANA M.Sc. Bioinformatics (IVth SEM) SESSION-2008-2009
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4. STRATEGY FOR RETRIEVING GENE/PROTIEN STANFORD MICROARRAY DATABASE PICK GENE OF UNKNOWN FUNCTION FROM MICROARRAY EXPERIMENTS IN HEPATITIS C(HUMAN) CHECK WHETHER IT IS AN EST OR NOT IN dbEST YES NO BLASTX OF THE SEQUENCE RETRIEVED FROM dbEST RETRIEVED UNAMED PROTEIN FROM BLASTX RESULTS General information about retrieved EST EST name:z076d04 GenBank Acc.-AA158258 EST length -625 bp
5. STRATEGY FOLLOWED FOR FUNCTION PREDICTION UNNAMED QUERY PROTEIN NCBI BLAST P(NR) CDD INTERPRO DATABASE HYDROPATHY PLOT PFAM DATABASE FUNCTION OF THE UNNAMED PROTEIN PREDICTED PSORT
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10. WORK SUMMARY Tool/software/server used Comment/Conclusion BLASTP (database: nr) SET-domain containing protein-methyltansferase. CDD database No domain identified * Interpro database No significant result * Psort tool Cytoplasm Hydropathy plot Pfam database String tool Cytoplasmic localization * Three families were identified No interaction * Conclusion for primary sequence analysis: Protein is Cytoplasmic and may play function as methyltansferase. * - Indicates data not shown for particular results.
11. STRATEGY FOLLOWED FOR HOMOLOGY MODELING Selection of template Alignment of target and template Model generation Validation of model Template selected : crystal structure of human cytosolic phospholipase A2 PDB ID:1CJY Seq identity: 33% w.r.t BAB71591 Length of template : 749 residues Length of BAB71591:165 aa
12. To predict the model for protein BAB71591 using modeller Url: http:// www.salilab.org/modeller/download_installation.html Comments : Predicted model shows 3 helices and 2 beta sheets and coils. Predicted model for BAB71591