1. Spectroscopy: Calibration curv
By Wilfredo Crespo
The purpose of this seminar was to learn how to make a calibration curve to obtain a
standard analysis of samples when using spectroscopy. Spectroscopy is the study of the
interaction between matter and radiated energy. In this seminar, we used the absorbance
method to know the concentration of an analyte (sample). This method consists of passing a
source of light through a sample and measuring how much light is absorbed by this sample. The
absorbance value is proportional to the concentration of the analyte. In order to do the
calibration curve, we prepared a stock solution of potassium permanganate. (Then, a dilute
solution was made, and different concentrations were obtained from it in each of the 11 flasks we
used. This sentence is not clear. Re-write it.) We again made dilute solutions from the last
diluted solution and each of them was put in an absorbance spectroscopy machine for analysis.
After obtaining the concentration numbers for each solution, we constructed a calibration curve
in Excel. In conclusion, I refreshed my memory about some things I knew related to spectroscopy,
and I also learned new things. In addition, I used some laboratory instruments that I had not ever
used before.