8. principles of biosafety, biocontainment & program management (nbb160)

Biocontainment LaboratoryBiosafety & Biocontainment Training,[object Object]

Principles of Biosafety,Biocontainment& Program Management,[object Object]

Learning Outcomes,[object Object],On successful completion of the course, the,[object Object],participant will be able to: ,[object Object],Discuss the components of the BMBL 5th edition,[object Object],Discuss characteristics of BSL- 1, BSL-2, BSL-3, and BSL-4 laboratories,[object Object],Discuss and demonstrate standard laboratory practices as applied in BSL- 1, BSL-2, BSL-3, and BSL-4 laboratories,[object Object]

What Do You Think?,[object Object],Is the laboratory a hazardous work environment?,[object Object],2. Do you work with hazardous materials, i.e. biological, chemical?,[object Object],Then, why do people choose to work without gloves, safety glasses, buttoned lab coats etc. in the laboratory? ,[object Object]

What’s Wrong With This Picture?,[object Object]

Historical Perspective,[object Object]

Historical Perspective,[object Object],First Recorded Laboratory-associated Infections,[object Object],Disease/agent Transmission Year,[object Object],Typhoid fever (Salmonella typhi) unknown 1885,[object Object],Tetanus (Clostridium tetani) syringe 1893,[object Object],Cholera (Vibriocholerae) pipette 1894,[object Object],Brucellosis (Brucellamelitensis) syringe 1897,[object Object],Diphtheria (Corynebacteriumdiphtheriae) pipette 1898,[object Object],Glanders (Burkholderiamallei) syringe 1898,[object Object]

Historical Perspective,[object Object],Fort Detrick, MD,[object Object],[object Object]

1943 - Developed Class III BSC prototype

1950 - Installed first Class I BSC

1954 - First Biological Safety Conference

Dr Arnold Wedum - director of safety from 1944 to 1969,[object Object]

Agent Laboratory-associated Infections,[object Object],Brucella sp. 507,[object Object],Coxiella burnetii 456,[object Object],Mycobacterium tuberculosis 417,[object Object],Hepatitis viruses 380,[object Object],Salmonella sp. 324,[object Object],Francisella tularensis 225,[object Object],Hantavirus 169,[object Object],Venezuelan equine ,[object Object], encephalomyelitis virus 150,[object Object],(Influenza A2 virus) (15),[object Object],Laboratory Infections: Most Frequent (1930-1999),[object Object]

Laboratory Accidents,[object Object], Splashes, spills - 27%,[object Object], Needlesticks - 25%,[object Object], Cuts - 16%,[object Object], Animal bites, scratches - 14%,[object Object], Mouth pipetting - 13%,[object Object], Unknown/other - 5%,[object Object],Pike, 1976,[object Object]

Laboratory Hazards & Risk,[object Object],Hazards,[object Object],[object Object]

PeopleRisk,[object Object],[object Object]

InjectionHazard & risk must be communicated to ALL staff! ,[object Object]

Risk Assessment,[object Object],Contamination?,[object Object],Containment?,[object Object],The “risk assessment” is the backbone ,[object Object],of safe practice! ,[object Object]

Laboratory Safety Programs,[object Object],Biosafety Program,[object Object],[object Object]

Proficient in practices & techniques

Exposure control plans/programs

Safety committee(s),[object Object]

RelevantGet training for what you ACTUALLY ,[object Object],do not what others THINK you do! ,[object Object]

Regulations,[object Object],[object Object]

Possession, Use and Transfer of Select Agents and Toxins

US Government Principles for Utilization and Care of Vertebrate Animals Used in Testing, Research, and Training

Transportation of Dangerous Goods Acts and Regulations

Hazardous Waste Management Standard

Occupational Exposure to Hazardous Laboratory Chemicals Standard

Radiation Protection Standard,[object Object]

CDC/NIH Guidelines,[object Object],Biohazard,[object Object],An agent of biological origin that has the capacity to produce deleterious effects on humans, i.e. microorganisms, toxins and allergens derived from those organisms; and allergens and toxins derived from higher plants and animals.,[object Object]

CDC/NIH Guidelines,[object Object],Biohazard Classification: Biosafety Levels,[object Object],BSL1 Agents not known to cause disease,[object Object],BSL2 Agents associated with human disease,[object Object],BSL3 Indigenous/exotic agents associated,[object Object], with human disease and with potential,[object Object], for aerosol transmission,[object Object],BSL4 Dangerous/exotic agents of life,[object Object], threatening nature,[object Object]

CDC/NIH Guidelines,[object Object],Biosafety,[object Object],The application of combinations of laboratory practice and procedure, laboratory facilities, and safety equipment when working with potentially infectious microorganisms.,[object Object]

CDC/NIH Guidelines,[object Object],Laboratory Classification: Biosafety Levels,[object Object],Combinations of laboratory practice and procedure, laboratory facilities, and safety equipment to provide containment ,[object Object],Low Containment,[object Object],Biosafety Level 1,[object Object],Biosafety Level 2 ,[object Object],Biosafety Level 3,[object Object],Biosafety Level 4 ,[object Object],High Containment,[object Object]

CDC/NIH Guidelines,[object Object],Containment,[object Object],[object Object],[object Object]

Good microbiological technique

Appropriate safety equipment,[object Object]

Operational practices,[object Object]

Special Safety equipment,[object Object],[object Object]

Special Facilities,[object Object],[object Object]

Standard microbiological practices

No special primary or secondary barriers recommended (other than a sink for hand washing)

No special practices,[object Object]

Adequate illumination,[object Object]

Biosafety Level 2 (BSL-2),[object Object],[object Object], and agents (i.e. Hepatitis B virus, HIV,,[object Object], Salmonella, etc.) ,[object Object],[object Object]

Secondary barriers (i.e. hand washing sinks, segregated waste, BSCs available

Special practices (i.e. immunization program, access controls),[object Object]

Sinks with hands-free operation

Impervious bench tops resistant to moderate heat & organic solvents,[object Object],[object Object]

BSCs installed properly; away from doors,[object Object]

Biosafety Level 3 (BSL-3),[object Object],[object Object], potential for respiratory transmission; may,[object Object], cause serious or lethal infection (i.e.,[object Object],Mycobacterium tuberculosis, St. Louis,[object Object], encephalitis virus, and Coxiella burnetii) ,[object Object],[object Object]

Secondary barriers (i.e. BSCs required, directional air flow, anti-room entry)

Special practices (i.e. restricted access, solid front lab coat, autoclave required),[object Object]

8. principles of biosafety, biocontainment & program management (nbb160)

8. principles of biosafety, biocontainment & program management (nbb160)

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