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Endophytic fungi: lipase activity and biotechnological applications
Brazilian Meeting on Organic Synthesis – 16th
BMOS – November 15-18, 2015 - Búzios, Brazil
Endophytic fungi: Lipase Activity and Biotechnological
Karla S. C. da Rocha1*, Maria S. R. Queiroz1, Guilherme Maxwell1, Denise O.
Guimarães1, Ivaldo I. Junior2 e Ivana C. R. Leal1.
1Laboratório de Produtos Naturais e Ensaios Biológicos (LaProNEB)- CEP: 21941-902, Federal University of
Rio de Janeiro, Brazil. 2Department of Bioengineering, School of Chemistry - CEP: 21941-909 – Federal
University of Rio de Janeiro, Brazil.
Keywords: endophytic fungi, lipases, monoacyglycerols
Nowadays microbial lipases have great industrial
importance. However, a few papers have explored
the potential of endophytic fungi, micro-organisms
that apparently not cause any damage to their host1,
as a source of these enzymes. Our research group
identified and isolated many endophytic fungi
associated with some plant species, which may
represent important sources of promising lipases.
Monoacylglycerols (MAGs) are amphiphilic
molecules that have applications such as
emulsifiers, solubility agents and chiral building
blocks. They are currently produced by alkaline
glycerolysis oil under high P and T, resulting in low
yields and numerous by-products. The use of
enzymatic processes catalyzed by lipase, may lead
to a more ecological approach2. This work aimed to
investigate lipase activity of endophytic fungi as well
as their application as biocatalysts in esterification
reactions for esters production.
RESULTS AND DISCUSSION
An initial screening of lipase activity was initially held
by the Rhodamine B (0,001%) method, at 365 nm of
absorbance. The HB13 strain presented
fluorescence halo after 24h of inoculation,
confirming the lipase activity. Lipolytic fungi were
inoculated into a pre-fermentation medium and
incubated for 24 h at 30 °C, and then transferred into
a fermentation medium to stimulate the production of
lipase, incubated for 48h, 30°C and 180rpm of
stirring. From the fermentation medium was
performed palmitate hydrolysis of p-nitrophenyl
method (pPNN). The enzyme activity was defined as
the concentration of p-nitrophenol in mol/L, based on
the molar absorption coefficient of p-nitrophenol
obtained from a calibration curve (R2 = 0.97) at 410
nm. The best result was obtained in 48h of
incubation with the HB13 and TB1fungus (Graphic
Figure 1. Medium with HB13 fungus
containing Rhodamine B fluorescing
Graphic1. p-nitrophenol concentration: HB13 (92U/mL);
TB1 (85U/ml); TB2 (5U/mL).
For the assays Rhodamine B and pPNN TB1 and
HB13 strain were promising for lipase activity.
Promising species will be investigated for the
esterification of activity, and will later be submitted to
lipase and MAGs production reactions.
CAPES, CNPq, FINEP and FAPERJ.
1. SCHULZ, B.; BOYLE, C. (2005). Mycol Res 109:661–686; 2. JUNIOR, I.I.;
FLORES, M.C.; SUTILI, F.K.; LEITE, S.G.F.; MIRANDA, L.S.; LEAL, I.C.R. DE
SOUZA, R.O.M.A. (2012). Journal of Mol Cat. B, Enz., 77:53– 58.