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IB Biology
2 Cells
2.3 Eukaryotic Cells
All syllabus statements ©IBO 2007
All images CC or public domain or link to original material.

Jason de Nys




                                                               http://commons.wikimedia.org/wiki/File:Biological_cell.svg
2.3.1.
Draw and label a diagram of the ultrastructure of a liver cell as an example of an animal cell
2.3.2 Annotate the diagram from 2.3.1 with the functions of each named structure.

  The Nucleus contains
  the chromosomes
  which comprise most
  of the DNA in a cell
  - It is the largest
  organelle
  - It has a double layer
  membrane
  - mRNA, transcribed
  from the DNA in the
  nucleus, exits through
  pores more in 3.3, 3.4 and 7.1 and 7.2
  - Some cells have
  multiple nuclei                             The bright blue stains are nuclei in HeLa
                                              cells. Read “The Immortal Life of
                                              Henrietta Lacks” for a fascinating story
                                              of the origin of HeLa cells
http://commons.wikimedia.org/wiki/File:HeLa_cells_stained_with_Hoechst_33258.jpg
http://commons.wikimedia.org/wiki/File:Diagram_human_cell_nucleus.svg
The Cell membrane is the boundary of the cell.
• It acts as a “gatekeeper”, preventing the entry or exit of some
  molecules and facilitating the movement of others.
• It is a phospholipid bilayer
• It is permeable to oxygen and carbon dioxide
• It is impermeable to water and charged particles, they must
  enter through special proteins embedded in the membrane
                                                                          More in 2.4




                        http://commons.wikimedia.org/wiki/File:Cell_membrane_detailed_diagram_en.svg
The Mitochondrion (pl. Mitochondria)
• The ‘power house’ of the cell
• Has a smooth outer membrane and
  a folded inner membrane
• Where aerobic respiration occurs in
  the cell
       More in 3.7 and 8.1


                                                            Mitochondria in mammalian lung cells




                                                             Remember:
                                                             Where else do we see loops of DNA?
                                                             How does the size of a mitochondrion
                                                             compare with an average prokaryote?

                                                             The implications of the answers to these
                                                             questions are in Option D: Evolution




                             http://commons.wikimedia.org/wiki/File:Animal_mitochondrion_diagram_en.svg
                             http://commons.wikimedia.org/wiki/File:Mitochondria,_mammalian_lung_-_TEM.jpg
Rough Endoplasmic Reticulum


     Spot the
    difference?


 Smooth Endoplasmic Reticulum




                                http://images.wellcome.ac.uk/
The ‘spots’ are the
        difference!

The Rough Endoplasmic
Reticulum is peppered with
ribosomes that give it the
rough appearance

It is where protein synthesis
occurs
               more in 3.5 and 7.4
The (free) Ribosome, the
molecular machine
responsible for protein
synthesis

       much, much more in 3.5 and 7.4




                               A ribosome on
                               the sculpture
                               “Waltz of the
                               Polypeptides” at Cold Spring Harbor Laboratory


                            http://www.flickr.com/photos/cryo_mariena/6033827307/sizes/m/in/photostream/
I shall name it………
    The internal reticular apparatus!!
           Pretty catchy… no?*




Camillo
Golgi                                                 *Everybody thought that was a terrible
                                                      name, so they called it the Golgi
                                                      apparatus instead
                                                                         http://commons.wikimedia.org/wiki/File:C_Golgi.jpg
          http://commons.wikimedia.org/wiki/File:Golgi_in_the_cytoplasm_of_a_macrophage_in_the_alveolus_(lung)_-_TEM.jpg
The Golgi Apparatus is a flattened
stack of membranes responsible
for the packaging and delivery of
proteins




                                     http://en.wikipedia.org/wiki/File:Nucleus_ER_golgi.svg
Lysosomes are simple, membrane-bound organelles
full of enzymes that digest engulfed bacteria and
viruses and large molecules
for recycling.




                                 http://commons.wikimedia.org/wiki/File:Lysosome.jpg
Image from an amazing site by teacher Andrew Brown
http://www.tokresource.org/tok_classes/biobiobio/biomenu/eukaryotic_cells/index.htm
2.3.3 Identify structures from 2.3.1 in electron micrographs of liver cells.




  Rough Endoplasmic Reticulum



  Mitochondrion




    Smooth Endoplasmic Reticulum
What can you see?
2.3.4 Compare prokaryotic and eukaryotic cells
   Compare Give an account of similarities and differences between
    two (or more) items, referring to both (all) of them throughout
                     Prokaryotic                                  Eukaryotic
     Small cells                                 Relatively larger cells
     Always unicellular                          Some multicellular, some unicellular
     No nucleus: DNA a ‘naked’ loop in the       DNA in chromosomes in a membrane-
     nucleoid region                             bound nucleus
     Ribosomes smaller (70s)                     Ribosomes larger (80s)
     No mitochondria, respiration in cell        Mitochondria, where aerobic respiration
     membrane and mesosomes                      occurs
     Cell division by binary fission             Cell division by meiosis or Mitosis
     Reproduction asexual (some gene             Reproduction Sexual or asexual
     exchange can occur via conjugation)




                                                             Table modified from Click4Biology
2.3.5 State three differences between plant and animal cells

  State: Give a specific name, value or other brief answer without
  explanation or calculation.
                           Plants                                          Animals
   Have a cell wall                                     Don’t have a cell wall
   Have chloroplasts in photosynthetic cells            Don’t have chloroplasts anywhere
   Carbohydrate stored as starch and plant
   oils                                            V.   Carbohydrate stored as glycogen and
                                                        animal fat
   Rigid Shape (due to cell wall)                       Flexible shape
   Have a large permanent storage vacuole               May have small, temporary vacuoles




http://www.flickr.com/photos/chubbybat/45407031/                    http://www.flickr.com/photos/powi/749366522/
2.3.6 Outline two roles of extracellular components


                                                      Bone cells have an
       Outline:
                                                      extracellular matrix in
      Give a brief
      account or                                      the interstitial spaces
      summary.                                        (between the cells)of
       Got a banana?                                  collagen and calcium
                                                      phosphate; which
                                                      together form the hard
                                                      bone.




                                         http://www.flickr.com/photos/limonada/14705232/
The other form of
extracellular matrix is the
basement membranes
                                        They exist in many tissue
                                        types as a form of support
                                        e.g. as the lining in blood
                                        vessels


You may already know about the
glomerulus in the kidney. A basement
membrane is integral to ultrafiltration
there.

More in HL 11.3



   http://commons.wikimedia.org/wiki/File:Gallbladder_cholesterolosis_low_mag.jpg
As well as extracellular
matrices in animals,
plant have extracellular
components….
Cell Walls


         They are made of cellulose and provide
         structure, support and protection. They
                      maintain cell shape and
                      prevent turgor pressure
                      from rupturing the cell

                  http://www.flickr.com/photos/ah_pao/2590017159/
Further information:




                       Three of the best sites for
                       IB-specific Biology
                       information. The top link
                       takes you to the PPT by
                       Stephen Taylor

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IB Biology Core 2.3: Eukaryotic Cells

  • 1. IB Biology 2 Cells 2.3 Eukaryotic Cells All syllabus statements ©IBO 2007 All images CC or public domain or link to original material. Jason de Nys http://commons.wikimedia.org/wiki/File:Biological_cell.svg
  • 2. 2.3.1. Draw and label a diagram of the ultrastructure of a liver cell as an example of an animal cell
  • 3. 2.3.2 Annotate the diagram from 2.3.1 with the functions of each named structure. The Nucleus contains the chromosomes which comprise most of the DNA in a cell - It is the largest organelle - It has a double layer membrane - mRNA, transcribed from the DNA in the nucleus, exits through pores more in 3.3, 3.4 and 7.1 and 7.2 - Some cells have multiple nuclei The bright blue stains are nuclei in HeLa cells. Read “The Immortal Life of Henrietta Lacks” for a fascinating story of the origin of HeLa cells http://commons.wikimedia.org/wiki/File:HeLa_cells_stained_with_Hoechst_33258.jpg
  • 5. The Cell membrane is the boundary of the cell. • It acts as a “gatekeeper”, preventing the entry or exit of some molecules and facilitating the movement of others. • It is a phospholipid bilayer • It is permeable to oxygen and carbon dioxide • It is impermeable to water and charged particles, they must enter through special proteins embedded in the membrane More in 2.4 http://commons.wikimedia.org/wiki/File:Cell_membrane_detailed_diagram_en.svg
  • 6. The Mitochondrion (pl. Mitochondria) • The ‘power house’ of the cell • Has a smooth outer membrane and a folded inner membrane • Where aerobic respiration occurs in the cell More in 3.7 and 8.1 Mitochondria in mammalian lung cells Remember: Where else do we see loops of DNA? How does the size of a mitochondrion compare with an average prokaryote? The implications of the answers to these questions are in Option D: Evolution http://commons.wikimedia.org/wiki/File:Animal_mitochondrion_diagram_en.svg http://commons.wikimedia.org/wiki/File:Mitochondria,_mammalian_lung_-_TEM.jpg
  • 7. Rough Endoplasmic Reticulum Spot the difference? Smooth Endoplasmic Reticulum http://images.wellcome.ac.uk/
  • 8. The ‘spots’ are the difference! The Rough Endoplasmic Reticulum is peppered with ribosomes that give it the rough appearance It is where protein synthesis occurs more in 3.5 and 7.4
  • 9. The (free) Ribosome, the molecular machine responsible for protein synthesis much, much more in 3.5 and 7.4 A ribosome on the sculpture “Waltz of the Polypeptides” at Cold Spring Harbor Laboratory http://www.flickr.com/photos/cryo_mariena/6033827307/sizes/m/in/photostream/
  • 10. I shall name it……… The internal reticular apparatus!! Pretty catchy… no?* Camillo Golgi *Everybody thought that was a terrible name, so they called it the Golgi apparatus instead http://commons.wikimedia.org/wiki/File:C_Golgi.jpg http://commons.wikimedia.org/wiki/File:Golgi_in_the_cytoplasm_of_a_macrophage_in_the_alveolus_(lung)_-_TEM.jpg
  • 11. The Golgi Apparatus is a flattened stack of membranes responsible for the packaging and delivery of proteins http://en.wikipedia.org/wiki/File:Nucleus_ER_golgi.svg
  • 12. Lysosomes are simple, membrane-bound organelles full of enzymes that digest engulfed bacteria and viruses and large molecules for recycling. http://commons.wikimedia.org/wiki/File:Lysosome.jpg
  • 13. Image from an amazing site by teacher Andrew Brown http://www.tokresource.org/tok_classes/biobiobio/biomenu/eukaryotic_cells/index.htm
  • 14. 2.3.3 Identify structures from 2.3.1 in electron micrographs of liver cells. Rough Endoplasmic Reticulum Mitochondrion Smooth Endoplasmic Reticulum
  • 15. What can you see?
  • 16. 2.3.4 Compare prokaryotic and eukaryotic cells Compare Give an account of similarities and differences between two (or more) items, referring to both (all) of them throughout Prokaryotic Eukaryotic Small cells Relatively larger cells Always unicellular Some multicellular, some unicellular No nucleus: DNA a ‘naked’ loop in the DNA in chromosomes in a membrane- nucleoid region bound nucleus Ribosomes smaller (70s) Ribosomes larger (80s) No mitochondria, respiration in cell Mitochondria, where aerobic respiration membrane and mesosomes occurs Cell division by binary fission Cell division by meiosis or Mitosis Reproduction asexual (some gene Reproduction Sexual or asexual exchange can occur via conjugation) Table modified from Click4Biology
  • 17. 2.3.5 State three differences between plant and animal cells State: Give a specific name, value or other brief answer without explanation or calculation. Plants Animals Have a cell wall Don’t have a cell wall Have chloroplasts in photosynthetic cells Don’t have chloroplasts anywhere Carbohydrate stored as starch and plant oils V. Carbohydrate stored as glycogen and animal fat Rigid Shape (due to cell wall) Flexible shape Have a large permanent storage vacuole May have small, temporary vacuoles http://www.flickr.com/photos/chubbybat/45407031/ http://www.flickr.com/photos/powi/749366522/
  • 18. 2.3.6 Outline two roles of extracellular components Bone cells have an Outline: extracellular matrix in Give a brief account or the interstitial spaces summary. (between the cells)of Got a banana? collagen and calcium phosphate; which together form the hard bone. http://www.flickr.com/photos/limonada/14705232/
  • 19. The other form of extracellular matrix is the basement membranes They exist in many tissue types as a form of support e.g. as the lining in blood vessels You may already know about the glomerulus in the kidney. A basement membrane is integral to ultrafiltration there. More in HL 11.3 http://commons.wikimedia.org/wiki/File:Gallbladder_cholesterolosis_low_mag.jpg
  • 20. As well as extracellular matrices in animals, plant have extracellular components…. Cell Walls They are made of cellulose and provide structure, support and protection. They maintain cell shape and prevent turgor pressure from rupturing the cell http://www.flickr.com/photos/ah_pao/2590017159/
  • 21. Further information: Three of the best sites for IB-specific Biology information. The top link takes you to the PPT by Stephen Taylor