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ENERGY RICH COMPOUNDS
• Energy-rich compounds: Such compounds in biological system whose ΔG0’ of hydrolysis yields free energy equal to or greater
than -25 kJ/mol are called energy-rich compounds or high-energy compounds, while, those whose ΔG0’ of hydrolysis yields
free less than -25 kJ/mol are called low-energy compounds.
• Most of the high-energy compounds contain phosphate group (viz., phosphoanhydride, enol phosphates, acyl phosphates and
guanido phosphates) are also called high-energy phosphates having high phophoryl transfer potential except thiol esters that
have high acyl transfer potential.
• High-energy bonds: The bonds in the energy-rich compounds, which yields high energy upon hydrolysis are called high-
energy bonds. A squiggle ‘~’ is used to indicate energy-rich bonds. In 1941 Fritz Lipmann introduced this convention.
• The term "high-energy bond," is however incorrect and misleading because it wrongly suggests that the bond itself contains
the energy. The fact is that breaking of chemical bonds requires an input of energy. The free energy released by hydrolysis of
high-energy compounds results from the products of the reaction having a smaller free-energy content than the reactants and
that’s not from breaking of any specific bond.
• ΔG⁰` - It is the difference in free energies of the products and reactants.
Energy Rich Compounds
S. No.
Energy-rich
compounds
Energy-rich bond Example
ΔG⁰`
(kJ/mol) (kcal/mol)
1 Pyrophosphates
Acid anhydride
(formed by condensation of acid groups or its
derivatives)
– C–P~P ATP to ADP -30.5 -7.3
2 Enol phosphates
Enol phosphate
(It is formed when phosphate group attaches to a
hydroxyl group which is bounded to a carbon atom
having double bond.)
– CH
║
– C–O~P
Phosphoenol pyruvate -61.9 -14.8
3 Acyl phosphates
Bond between carboxy and phosphate
(The high energy bond in this compound is formed by
the reaction between carboxylic acid group and
phosphate group)
O
║
– C–O~P
1,3-
bisphosphoglycerate
-49.4 -11.8
4 Thiol esters
Thioester
(Thioester bond results from the reaction between thiol
and carboxylic acid group)
O
║
– C~S –
Acetyl CoA -31.4 -7.5
5
Guanido
phosphates
(phophagens)
Guanidine phosphate bond
(It is formed by the attachment of phosphate group to
guanidine group)
|
– N~P Phosphocreatine -43.1 -10.3
Classification of Energy-rich compounds: Based on type of bonds
Structure of some energy-rich compounds
1,3-bisphosphoglycerate
Acetyl CoA
Phosphoenol phosphate
Phosphocreatine
ATP
~
~
O
~
~ ~
~ ~
~
-
What makes these compounds energy-rich?
Resonance stabilization, solvation and/or electrostatic repulsion
Let’s have a look at the structure of individual energy-rich compound and their
hydrolytic product…
Adenosine triphosphate (ATP)
• ATP is a nucleotide made up of adenine, ribose sugar and a triphosphate group (viz., α, β and γ designated from
C5’).
• Adenine is linked to C1’ of sugar via N-glycosidic bond.
• α- phosphate is attached to C’5 of sugar via phosphoester bond, while β- and γ- phosphate via phosphoanhydride
bond.
• It is a energy rich compound because it contains 2 energy-rich phosphoanhydride bonds, one between α- and β-,
while second between β- and γ- phosphoryl groups.
•
γ β α
Adenine
Ribose sugar
Triphosphate groups
N-glycosidic bond
Phosphoanhydride bond Phosphoester bond
Fig.1. Structure of ATP
9
1’
5’
4’
3’ 2’
4
3
2
1
5
6
7
8
Adenosine triphosphate (ATP)
~ ~
• ATP hydrolysis is highly exergonic i.e., a large amount of free energy is liberated when ATP is hydrolyzed to
adenosine diphosphate (ADP) and orthophosphate (Pi) or when ATP is hydrolyzed to adenosine
monophosphate (AMP) and pyrophosphate (PPi ).
• While, the synthesis of ATP by phosphorylation of ADP is a highly endergonic reaction.
ATP4¯ + H2O ADP 3¯ + Pi2¯ + H +
ΔG⁰` = (+7.3 kcal/mol)
ATP4¯ + H2O AMP2¯+ PPi3¯ + H +
ΔG⁰` = (-10.9 kcal/mol)
ATP hydrolysis is highly exergonic
ΔG⁰` = -7.3 kcal/mol
• Since ΔG⁰` is the difference of free energy of reactant (ATP) and product (ADP & Pi), so structure of both need to
be examined to understand this.
• 3 factors responsible for making ATP hydrolysis exergonic i.e. high phosphoryl transfer potential are: 1. Resonance
stabilization 2. Electrostatic repulsion 3. Stabilization due to hydration
What makes ATP hydrolysis exergonic?
ΔG⁰` = (+10.9 kcal/mol)
1. Resonance stabilization:
•Products (ADP and Pi) has greater resonance stabilization than that of reactant (ATP).
•Pi has a number of resonance forms of similar energy, whereas the γ- phosphoryl group of ATP has a smaller number.
Fig.2B. Improbable resonance structure of terminal part of ATP
AMP
γ
β
B.
A.
cont.
Fig.2A. Resonance structure of orthophosphate (Pi)
2. Electrostatic repulsion:
• Improbable resonance structure of terminal part of ATP (Fig. 2B) is unfavorable because a +vely charged O-atom is
adjacent to a +vely charged P-atom, an electrostatically unfavorable juxtaposition.
• ATP carries 4 –ve charges in close proximity (Fig 1.) at neutral pH leading to electrostatic charge repulsion. Release
of the terminal phosphate group removes an unfavorable repulsion between adjacent negative charges. This
repulsion is reduced by hydroxylation.
3. Stabilization due to hydration:
• Water can bind more effectively to ADP and Pi than it can to the phosphoanhydride part of ATP, stabilizing the ADP
and Pi by hydration.
• In other words solvation of products (ADP and Pi ) or (AMP and PPi ) is better than solvation of ATP.
cont.
Phosphoenol pyruvate (PEP)
•Reasons that favors hydrolysis of PEP to pyruvate:
•Electrostatic charge repulsion between negatively charged oxygen of phosphoryl group and carboxyl group favors removal of Pi.
•Resonance stabilization of hydrolytic product Pi.
•Isomerization (tautomerization) of hydrolytic product pyruvate into enol- keto form.
1,3-Bisphosphoglycerate (1,3-BPA)
•Reasons that favors hydrolysis of 1,3-BGA to 3-Phosphoglyceraldehyde:
•Resonance stabilization of hydrolytic product Pi.
• Release of proton from hydrolytic product 3-phosphoglyceric acid to maintain pH of cell.
•Resonance stabilization of carboxylic group of 3-phosphoglycerate.
Acetyl CoA
•Reasons that favors hydrolysis of phosphocreatine to creatine: Hydrolytic removal of CoA from Acetyl CoA favors ionization
and resonance stabilization of carboxylic group of the hydrolytic product.
Phosphocreatine
•Reasons that favors hydrolysis of phosphocreatine to creatine:
•Resonance stabilization of hydrolytic product Pi.
•Resonance stabilization of hydrolytic product creatine.
~
~
ATP : UNIVERSAL ENERGY COULER
Intermediate position in phosphate-rich compounds
• Phosphate transfer potential: It is defined as the negative of the standard free energy change (ΔG0’) for the hydrolytic
removal of phosphate from the phosphorylated compounds.
• Phosphates can be transferred from compounds with higher ΔG0’. to those with lower ΔG0’.
• ATP occupies an intermediate position in overall spectrum of energy-rich phosphorylated compounds (i.e., compounds
with high phosphoryl transfer potential (Table 1).
• Compounds viz., phosphoenolpyruvate (PEP), 1,3-bisphosphoglycerate (1,3-BPG), and creatine phosphate have a higher
phosphoryl-transfer potential than that of ATP, while glucose-6-phosphate, glycerol-3-phosphate, etc. have low potential.
Phosphoryl-transfer potential : Intermediate position of ATP
Phosphorylated Compound ΔG0‘ (kcal/mol)
Phosphoenolpyruvate -14.8
1,3-bisphosphoglycerate -11.8
Creatine phosphate -10.3
ATP (to ADP) -7.3
Glucose-1-phosphate -5.0
Pyrophosphate -4.6
Glucose-6-phosphate -3.3
Glycerol-3-phosphate -2.2
Table1. Standard free energy of some phosphorylated compound
High
Energy
Comps
Low
Energy
comps
• This intermediate position ATP makes it an efficient carrier of phosphoryl groups, where ATP can act as a phosphate donor
and ADP, its dephosphorylated form can act as a phosphate acceptor linking energy producing and energy requiring reactions.
• Compounds possessing high-phosphoryl-transfer-potential (1,3-BPG, PEP, and creatine phosphate) derived from the metabolism
of fuel molecules can transfer their phosphoryl group to ADP to form ATP. This mechanism for making ATP is called
substrate-level phosphorylation.
• During glycolysis PEP and 1,3-BPG transfer its phosphoryl group to ADP to form ATP.
• Creatine phosphate serves as an energy reserve in vertebrate muscle. It is used to regenerate ATP from ADP every time we
exercise strenuously.
• In turn, ATP donates a phosphoryl group to form low energy compounds to facilitate their metabolism.
cont.
PEP + ADP + H+ Pyruvate + ATP
1,3-BPG + ADP+ H+ 3-phosphoglycerate + ATP
Phosphoglycerokinase
Pyruvatekinase
Phosphocreatine + ADP + H+ Creatine + ATP
Creatine kinase
Glucose + ATP Glucose-6-phosphate + ADP + H+
Hexokinase
Role of ATP/ADP System: An intermediate in cellular energy metabolism
• The free energy liberated during hydrolysis of ATP is harnessed to drive reactions that require an input of free
energy, such as muscle contraction. In turn, ATP is formed from ADP and Pi when fuel molecules are oxidized in
chemotrophs or when light is trapped by phototrophs. This ATP–ADP cycle is the fundamental mode of energy
exchange in biological systems.
• ATP is the major energy currency for cells, but it is not the only energy currency.
• Other nucleoside triphosphates (NTPs) have major roles in metabolism. Guanosine 5'-triphosphate (GTP) supplies
some of the energy used during protein synthesis. Cytidine 5'-triphosphate (CTP) is used during lipid synthesis, and
uridine 5'-triphosphate (UTP) is used for the synthesis of peptidoglycan and other polysaccharides.
Energy from Sunlight or food
i.e. phototrphy, chemotrphy,
arebic/anaerobic respiration,
fermentation
Energy available for cellular
work and for chemical synthesis
ATP
ADP + Pi
Fig. Cell’s energy cycle
THANK YOU

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Energy rich compounds.pdf

  • 2. • Energy-rich compounds: Such compounds in biological system whose ΔG0’ of hydrolysis yields free energy equal to or greater than -25 kJ/mol are called energy-rich compounds or high-energy compounds, while, those whose ΔG0’ of hydrolysis yields free less than -25 kJ/mol are called low-energy compounds. • Most of the high-energy compounds contain phosphate group (viz., phosphoanhydride, enol phosphates, acyl phosphates and guanido phosphates) are also called high-energy phosphates having high phophoryl transfer potential except thiol esters that have high acyl transfer potential. • High-energy bonds: The bonds in the energy-rich compounds, which yields high energy upon hydrolysis are called high- energy bonds. A squiggle ‘~’ is used to indicate energy-rich bonds. In 1941 Fritz Lipmann introduced this convention. • The term "high-energy bond," is however incorrect and misleading because it wrongly suggests that the bond itself contains the energy. The fact is that breaking of chemical bonds requires an input of energy. The free energy released by hydrolysis of high-energy compounds results from the products of the reaction having a smaller free-energy content than the reactants and that’s not from breaking of any specific bond. • ΔG⁰` - It is the difference in free energies of the products and reactants. Energy Rich Compounds
  • 3. S. No. Energy-rich compounds Energy-rich bond Example ΔG⁰` (kJ/mol) (kcal/mol) 1 Pyrophosphates Acid anhydride (formed by condensation of acid groups or its derivatives) – C–P~P ATP to ADP -30.5 -7.3 2 Enol phosphates Enol phosphate (It is formed when phosphate group attaches to a hydroxyl group which is bounded to a carbon atom having double bond.) – CH ║ – C–O~P Phosphoenol pyruvate -61.9 -14.8 3 Acyl phosphates Bond between carboxy and phosphate (The high energy bond in this compound is formed by the reaction between carboxylic acid group and phosphate group) O ║ – C–O~P 1,3- bisphosphoglycerate -49.4 -11.8 4 Thiol esters Thioester (Thioester bond results from the reaction between thiol and carboxylic acid group) O ║ – C~S – Acetyl CoA -31.4 -7.5 5 Guanido phosphates (phophagens) Guanidine phosphate bond (It is formed by the attachment of phosphate group to guanidine group) | – N~P Phosphocreatine -43.1 -10.3 Classification of Energy-rich compounds: Based on type of bonds
  • 4. Structure of some energy-rich compounds 1,3-bisphosphoglycerate Acetyl CoA Phosphoenol phosphate Phosphocreatine ATP ~ ~ O ~ ~ ~ ~ ~ ~ -
  • 5. What makes these compounds energy-rich? Resonance stabilization, solvation and/or electrostatic repulsion Let’s have a look at the structure of individual energy-rich compound and their hydrolytic product…
  • 6. Adenosine triphosphate (ATP) • ATP is a nucleotide made up of adenine, ribose sugar and a triphosphate group (viz., α, β and γ designated from C5’). • Adenine is linked to C1’ of sugar via N-glycosidic bond. • α- phosphate is attached to C’5 of sugar via phosphoester bond, while β- and γ- phosphate via phosphoanhydride bond. • It is a energy rich compound because it contains 2 energy-rich phosphoanhydride bonds, one between α- and β-, while second between β- and γ- phosphoryl groups. • γ β α Adenine Ribose sugar Triphosphate groups N-glycosidic bond Phosphoanhydride bond Phosphoester bond Fig.1. Structure of ATP 9 1’ 5’ 4’ 3’ 2’ 4 3 2 1 5 6 7 8 Adenosine triphosphate (ATP) ~ ~
  • 7. • ATP hydrolysis is highly exergonic i.e., a large amount of free energy is liberated when ATP is hydrolyzed to adenosine diphosphate (ADP) and orthophosphate (Pi) or when ATP is hydrolyzed to adenosine monophosphate (AMP) and pyrophosphate (PPi ). • While, the synthesis of ATP by phosphorylation of ADP is a highly endergonic reaction. ATP4¯ + H2O ADP 3¯ + Pi2¯ + H + ΔG⁰` = (+7.3 kcal/mol) ATP4¯ + H2O AMP2¯+ PPi3¯ + H + ΔG⁰` = (-10.9 kcal/mol) ATP hydrolysis is highly exergonic ΔG⁰` = -7.3 kcal/mol • Since ΔG⁰` is the difference of free energy of reactant (ATP) and product (ADP & Pi), so structure of both need to be examined to understand this. • 3 factors responsible for making ATP hydrolysis exergonic i.e. high phosphoryl transfer potential are: 1. Resonance stabilization 2. Electrostatic repulsion 3. Stabilization due to hydration What makes ATP hydrolysis exergonic? ΔG⁰` = (+10.9 kcal/mol)
  • 8. 1. Resonance stabilization: •Products (ADP and Pi) has greater resonance stabilization than that of reactant (ATP). •Pi has a number of resonance forms of similar energy, whereas the γ- phosphoryl group of ATP has a smaller number. Fig.2B. Improbable resonance structure of terminal part of ATP AMP γ β B. A. cont. Fig.2A. Resonance structure of orthophosphate (Pi)
  • 9. 2. Electrostatic repulsion: • Improbable resonance structure of terminal part of ATP (Fig. 2B) is unfavorable because a +vely charged O-atom is adjacent to a +vely charged P-atom, an electrostatically unfavorable juxtaposition. • ATP carries 4 –ve charges in close proximity (Fig 1.) at neutral pH leading to electrostatic charge repulsion. Release of the terminal phosphate group removes an unfavorable repulsion between adjacent negative charges. This repulsion is reduced by hydroxylation. 3. Stabilization due to hydration: • Water can bind more effectively to ADP and Pi than it can to the phosphoanhydride part of ATP, stabilizing the ADP and Pi by hydration. • In other words solvation of products (ADP and Pi ) or (AMP and PPi ) is better than solvation of ATP. cont.
  • 10. Phosphoenol pyruvate (PEP) •Reasons that favors hydrolysis of PEP to pyruvate: •Electrostatic charge repulsion between negatively charged oxygen of phosphoryl group and carboxyl group favors removal of Pi. •Resonance stabilization of hydrolytic product Pi. •Isomerization (tautomerization) of hydrolytic product pyruvate into enol- keto form.
  • 11. 1,3-Bisphosphoglycerate (1,3-BPA) •Reasons that favors hydrolysis of 1,3-BGA to 3-Phosphoglyceraldehyde: •Resonance stabilization of hydrolytic product Pi. • Release of proton from hydrolytic product 3-phosphoglyceric acid to maintain pH of cell. •Resonance stabilization of carboxylic group of 3-phosphoglycerate.
  • 12. Acetyl CoA •Reasons that favors hydrolysis of phosphocreatine to creatine: Hydrolytic removal of CoA from Acetyl CoA favors ionization and resonance stabilization of carboxylic group of the hydrolytic product.
  • 13. Phosphocreatine •Reasons that favors hydrolysis of phosphocreatine to creatine: •Resonance stabilization of hydrolytic product Pi. •Resonance stabilization of hydrolytic product creatine. ~ ~
  • 14. ATP : UNIVERSAL ENERGY COULER Intermediate position in phosphate-rich compounds
  • 15. • Phosphate transfer potential: It is defined as the negative of the standard free energy change (ΔG0’) for the hydrolytic removal of phosphate from the phosphorylated compounds. • Phosphates can be transferred from compounds with higher ΔG0’. to those with lower ΔG0’. • ATP occupies an intermediate position in overall spectrum of energy-rich phosphorylated compounds (i.e., compounds with high phosphoryl transfer potential (Table 1). • Compounds viz., phosphoenolpyruvate (PEP), 1,3-bisphosphoglycerate (1,3-BPG), and creatine phosphate have a higher phosphoryl-transfer potential than that of ATP, while glucose-6-phosphate, glycerol-3-phosphate, etc. have low potential. Phosphoryl-transfer potential : Intermediate position of ATP Phosphorylated Compound ΔG0‘ (kcal/mol) Phosphoenolpyruvate -14.8 1,3-bisphosphoglycerate -11.8 Creatine phosphate -10.3 ATP (to ADP) -7.3 Glucose-1-phosphate -5.0 Pyrophosphate -4.6 Glucose-6-phosphate -3.3 Glycerol-3-phosphate -2.2 Table1. Standard free energy of some phosphorylated compound High Energy Comps Low Energy comps
  • 16. • This intermediate position ATP makes it an efficient carrier of phosphoryl groups, where ATP can act as a phosphate donor and ADP, its dephosphorylated form can act as a phosphate acceptor linking energy producing and energy requiring reactions. • Compounds possessing high-phosphoryl-transfer-potential (1,3-BPG, PEP, and creatine phosphate) derived from the metabolism of fuel molecules can transfer their phosphoryl group to ADP to form ATP. This mechanism for making ATP is called substrate-level phosphorylation. • During glycolysis PEP and 1,3-BPG transfer its phosphoryl group to ADP to form ATP. • Creatine phosphate serves as an energy reserve in vertebrate muscle. It is used to regenerate ATP from ADP every time we exercise strenuously. • In turn, ATP donates a phosphoryl group to form low energy compounds to facilitate their metabolism. cont. PEP + ADP + H+ Pyruvate + ATP 1,3-BPG + ADP+ H+ 3-phosphoglycerate + ATP Phosphoglycerokinase Pyruvatekinase Phosphocreatine + ADP + H+ Creatine + ATP Creatine kinase Glucose + ATP Glucose-6-phosphate + ADP + H+ Hexokinase
  • 17. Role of ATP/ADP System: An intermediate in cellular energy metabolism • The free energy liberated during hydrolysis of ATP is harnessed to drive reactions that require an input of free energy, such as muscle contraction. In turn, ATP is formed from ADP and Pi when fuel molecules are oxidized in chemotrophs or when light is trapped by phototrophs. This ATP–ADP cycle is the fundamental mode of energy exchange in biological systems. • ATP is the major energy currency for cells, but it is not the only energy currency. • Other nucleoside triphosphates (NTPs) have major roles in metabolism. Guanosine 5'-triphosphate (GTP) supplies some of the energy used during protein synthesis. Cytidine 5'-triphosphate (CTP) is used during lipid synthesis, and uridine 5'-triphosphate (UTP) is used for the synthesis of peptidoglycan and other polysaccharides. Energy from Sunlight or food i.e. phototrphy, chemotrphy, arebic/anaerobic respiration, fermentation Energy available for cellular work and for chemical synthesis ATP ADP + Pi Fig. Cell’s energy cycle